Assessment Of Genotoxic Potential Of The Velika Morava River Basin
Аутори
Jovanović Marić, JovanaKolarević, Stoimir
Milošković, Aleksandra
Kojadinović, Nataša
Simić, Vladica
Dojčinović, Biljana
Kracun-Kolarevic, Margareta
Paunović, Momir
Kostić, Jovana
Sunjog, Karolina
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The Danube River is highly affected by human activity. Wastewaters discharging into the Danube River stream are one of the major problems, especially at Croatia-Serbia river section. The Velika Morava River is the greatest Serbian river and a significant tributary of the Danube River. Consequently, the water quality of the Velika Morava River could have a great impact on the water quality of the Danube River.
In this study, the level of genotoxic potential along the Velika Morava River Basin was evaluated by using a battery of in vitro and in situ bioassays. Within the in vitro approach, SOS/umuC test on Salmonella thyphimurium TA1535/pSK1002 and the comet assay on HepG2 cell line were employed for genotoxicity testing of native water samples. The level of DNA damage on erythrocytes of bleak specimens (Alburnus alburnus) was observed using the alkaline and Fpg modified comet and micronucleus assays within in situ testing. The concentration of toxic metals in fish tissues and physico-c...hemical water status were chosen as parameters of pollution pressure on selected sites.
Obtained results indicated lower sensitivity of selected in vitro tests when compared to in situ. Also, the tests using the in situ approach showed a range of sensitivity. Namely, the alkaline comet assay showed a greater potential than Fpg modified comet test and micronucleus assay in distinguishing between sites. According to results, application of complex bioassays battery was shown as a better choice in eco/genotoxicological studies than using only one bioassay. In that way, a holistic approach is appropriate for this type of study.
Кључне речи:
genotoxic potential / in vitro bioassays / in situ bioassays / holistic approach / water pollutionИзвор:
The 42nd IAD Conference 2018, Smolenice, Slovakia, 2018, 29-Издавач:
- Institute of Geography of SAS
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Jovanović Marić, Jovana AU - Kolarević, Stoimir AU - Milošković, Aleksandra AU - Kojadinović, Nataša AU - Simić, Vladica AU - Dojčinović, Biljana AU - Kracun-Kolarevic, Margareta AU - Paunović, Momir AU - Kostić, Jovana AU - Sunjog, Karolina AU - Vuković-Gačić, Branka PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2905 AB - The Danube River is highly affected by human activity. Wastewaters discharging into the Danube River stream are one of the major problems, especially at Croatia-Serbia river section. The Velika Morava River is the greatest Serbian river and a significant tributary of the Danube River. Consequently, the water quality of the Velika Morava River could have a great impact on the water quality of the Danube River. In this study, the level of genotoxic potential along the Velika Morava River Basin was evaluated by using a battery of in vitro and in situ bioassays. Within the in vitro approach, SOS/umuC test on Salmonella thyphimurium TA1535/pSK1002 and the comet assay on HepG2 cell line were employed for genotoxicity testing of native water samples. The level of DNA damage on erythrocytes of bleak specimens (Alburnus alburnus) was observed using the alkaline and Fpg modified comet and micronucleus assays within in situ testing. The concentration of toxic metals in fish tissues and physico-chemical water status were chosen as parameters of pollution pressure on selected sites. Obtained results indicated lower sensitivity of selected in vitro tests when compared to in situ. Also, the tests using the in situ approach showed a range of sensitivity. Namely, the alkaline comet assay showed a greater potential than Fpg modified comet test and micronucleus assay in distinguishing between sites. According to results, application of complex bioassays battery was shown as a better choice in eco/genotoxicological studies than using only one bioassay. In that way, a holistic approach is appropriate for this type of study. PB - Institute of Geography of SAS C3 - The 42nd IAD Conference 2018, Smolenice, Slovakia T1 - Assessment Of Genotoxic Potential Of The Velika Morava River Basin SP - 29 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2905 ER -
@conference{ author = "Jovanović Marić, Jovana and Kolarević, Stoimir and Milošković, Aleksandra and Kojadinović, Nataša and Simić, Vladica and Dojčinović, Biljana and Kracun-Kolarevic, Margareta and Paunović, Momir and Kostić, Jovana and Sunjog, Karolina and Vuković-Gačić, Branka", year = "2018", abstract = "The Danube River is highly affected by human activity. Wastewaters discharging into the Danube River stream are one of the major problems, especially at Croatia-Serbia river section. The Velika Morava River is the greatest Serbian river and a significant tributary of the Danube River. Consequently, the water quality of the Velika Morava River could have a great impact on the water quality of the Danube River. In this study, the level of genotoxic potential along the Velika Morava River Basin was evaluated by using a battery of in vitro and in situ bioassays. Within the in vitro approach, SOS/umuC test on Salmonella thyphimurium TA1535/pSK1002 and the comet assay on HepG2 cell line were employed for genotoxicity testing of native water samples. The level of DNA damage on erythrocytes of bleak specimens (Alburnus alburnus) was observed using the alkaline and Fpg modified comet and micronucleus assays within in situ testing. The concentration of toxic metals in fish tissues and physico-chemical water status were chosen as parameters of pollution pressure on selected sites. Obtained results indicated lower sensitivity of selected in vitro tests when compared to in situ. Also, the tests using the in situ approach showed a range of sensitivity. Namely, the alkaline comet assay showed a greater potential than Fpg modified comet test and micronucleus assay in distinguishing between sites. According to results, application of complex bioassays battery was shown as a better choice in eco/genotoxicological studies than using only one bioassay. In that way, a holistic approach is appropriate for this type of study.", publisher = "Institute of Geography of SAS", journal = "The 42nd IAD Conference 2018, Smolenice, Slovakia", title = "Assessment Of Genotoxic Potential Of The Velika Morava River Basin", pages = "29", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2905" }
Jovanović Marić, J., Kolarević, S., Milošković, A., Kojadinović, N., Simić, V., Dojčinović, B., Kracun-Kolarevic, M., Paunović, M., Kostić, J., Sunjog, K.,& Vuković-Gačić, B.. (2018). Assessment Of Genotoxic Potential Of The Velika Morava River Basin. in The 42nd IAD Conference 2018, Smolenice, Slovakia Institute of Geography of SAS., 29. https://hdl.handle.net/21.15107/rcub_rimsi_2905
Jovanović Marić J, Kolarević S, Milošković A, Kojadinović N, Simić V, Dojčinović B, Kracun-Kolarevic M, Paunović M, Kostić J, Sunjog K, Vuković-Gačić B. Assessment Of Genotoxic Potential Of The Velika Morava River Basin. in The 42nd IAD Conference 2018, Smolenice, Slovakia. 2018;:29. https://hdl.handle.net/21.15107/rcub_rimsi_2905 .
Jovanović Marić, Jovana, Kolarević, Stoimir, Milošković, Aleksandra, Kojadinović, Nataša, Simić, Vladica, Dojčinović, Biljana, Kracun-Kolarevic, Margareta, Paunović, Momir, Kostić, Jovana, Sunjog, Karolina, Vuković-Gačić, Branka, "Assessment Of Genotoxic Potential Of The Velika Morava River Basin" in The 42nd IAD Conference 2018, Smolenice, Slovakia (2018):29, https://hdl.handle.net/21.15107/rcub_rimsi_2905 .