Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities
Само за регистроване кориснике
2008
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciate...d hydrogen (H-center dot) atom.
Кључне речи:
spin-traps / hydrogen radical / Fenton reaction / EPR spectroscopy / DEPMPOИзвор:
Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 2008, 69, 5, 1354-1366Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/j.saa.2007.09.047
ISSN: 1386-1425
PubMed: 18255335
WoS: 000256180800011
Scopus: 2-s2.0-42149158251
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bačić, Goran AU - Spasojević, Ivan AU - Secerov, Bojana AU - Mojović, Miloš PY - 2008 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/287 AB - The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy T1 - Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities EP - 1366 IS - 5 SP - 1354 VL - 69 DO - 10.1016/j.saa.2007.09.047 ER -
@article{ author = "Bačić, Goran and Spasojević, Ivan and Secerov, Bojana and Mojović, Miloš", year = "2008", abstract = "The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy", title = "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities", pages = "1366-1354", number = "5", volume = "69", doi = "10.1016/j.saa.2007.09.047" }
Bačić, G., Spasojević, I., Secerov, B.,& Mojović, M.. (2008). Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy Pergamon-Elsevier Science Ltd, Oxford., 69(5), 1354-1366. https://doi.org/10.1016/j.saa.2007.09.047
Bačić G, Spasojević I, Secerov B, Mojović M. Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2008;69(5):1354-1366. doi:10.1016/j.saa.2007.09.047 .
Bačić, Goran, Spasojević, Ivan, Secerov, Bojana, Mojović, Miloš, "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 69, no. 5 (2008):1354-1366, https://doi.org/10.1016/j.saa.2007.09.047 . .