The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciate...d hydrogen (H-center dot) atom.
TY - JOUR
AU - Bačić, Goran
AU - Spasojević, Ivan
AU - Secerov, Bojana
AU - Mojović, Miloš
PY - 2008
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/287
AB - The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom.
PB - Pergamon-Elsevier Science Ltd, Oxford
T2 - Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
T1 - Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities
EP - 1366
IS - 5
SP - 1354
VL - 69
DO - 10.1016/j.saa.2007.09.047
ER -
@article{
author = "Bačić, Goran and Spasojević, Ivan and Secerov, Bojana and Mojović, Miloš",
year = "2008",
abstract = "The choice of the spin-trap that is to be applied in any EPR study represents the crossroad between a comprehensive investigation and an "ordinary" quantification of production of radicals. So, the scope of our study was to compare the performance of different spin-traps for qualitative analysis of radical-generating systems, and their ability to recognize previously unnoticed radicals. In addition, we present a brief account of the difficulties involved in the detection of oxygen-centered radicals in chemical and biological systems accompanied by the rationale for using the EPR spin-trapping technique in quantitative studies of such reactive species. Certain technical aspects of EPR experiments related to efficient trapping of free radicals in biochemical systems are also discussed. As an example we present here results obtained using EPR spectroscopy and the spin-trap DEPMPO, which show that the Fenton reaction, as well as various biological systems generate a previously unappreciated hydrogen (H-center dot) atom.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy",
title = "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities",
pages = "1366-1354",
number = "5",
volume = "69",
doi = "10.1016/j.saa.2007.09.047"
}
Bačić, G., Spasojević, I., Secerov, B.,& Mojović, M.. (2008). Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy
Pergamon-Elsevier Science Ltd, Oxford., 69(5), 1354-1366.
https://doi.org/10.1016/j.saa.2007.09.047
Bačić G, Spasojević I, Secerov B, Mojović M. Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities. in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy. 2008;69(5):1354-1366.
doi:10.1016/j.saa.2007.09.047 .
Bačić, Goran, Spasojević, Ivan, Secerov, Bojana, Mojović, Miloš, "Spin-trapping of oxygen free radicals in chemical and biological systems: New traps, radicals and possibilities" in Spectrochimica Acta Part A-Molecular and Biomolecular Spectroscopy, 69, no. 5 (2008):1354-1366,
https://doi.org/10.1016/j.saa.2007.09.047 . .
