Penicillamine prevents damaging redox in vitro interactions of bilirubin and copper
Autori
Bozic Cvijan, BojanaKorać Jačić, Jelena
Stanković, Dalibor
Stanić, Marina
Popovic Bijelic, Ana
Bogdanović Pristov, Jelena
Spasojević, Ivan
Bajcetic, Milica
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or
coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological
pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry.
Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to
inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions
with BR. Cu1+ undergoes spontaneous oxidation by O2 resulting in hydrogen peroxide accumulation and
hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects
on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species
by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further
examination of thera...peutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia
is needed.
Ključne reči:
Penicillamine / bilirubin / copperIzvor:
14th Serbian Congress of Farmacologists and 4th Serbian Congress of Clinical Pharmacology with international participation, 2019, 240-242Izdavač:
- Srpsko farmakološko društvo Beograd, Novi Sad
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - CONF AU - Bozic Cvijan, Bojana AU - Korać Jačić, Jelena AU - Stanković, Dalibor AU - Stanić, Marina AU - Popovic Bijelic, Ana AU - Bogdanović Pristov, Jelena AU - Spasojević, Ivan AU - Bajcetic, Milica PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2847 AB - Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed. PB - Srpsko farmakološko društvo Beograd, Novi Sad C3 - 14th Serbian Congress of Farmacologists and 4th Serbian Congress of Clinical Pharmacology with international participation T1 - Penicillamine prevents damaging redox in vitro interactions of bilirubin and copper EP - 242 SP - 240 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2847 ER -
@conference{ author = "Bozic Cvijan, Bojana and Korać Jačić, Jelena and Stanković, Dalibor and Stanić, Marina and Popovic Bijelic, Ana and Bogdanović Pristov, Jelena and Spasojević, Ivan and Bajcetic, Milica", year = "2019", abstract = "Toxic effects of unconjugated bilirubin (BR) in neonatal hyperbilirubinemia have been related to redox and/or coordinate interactions with Cu2+. However, the development and mechanisms of such interactions at physiological pH have not been resolved. This study shows that BR reduces Cu2+ to Cu1+ in 1:1 stoichiometry. Apparently, BR undergoes degradation, i.e. BR and Cu2+ do not form stable complexes. The binding of Cu2+ to inorganic phosphates, liposomal phosphate groups, or to chelating drug penicillamine, impedes redox interactions with BR. Cu1+ undergoes spontaneous oxidation by O2 resulting in hydrogen peroxide accumulation and hydroxyl radical production. In relation to this, copper and BR induced synergistic oxidative/damaging effects on erythrocytes membrane, which were alleviated by penicillamine. The production of reactive oxygen species by BR and copper represents a plausible cause of BR toxic effects and cell damage in hyperbilirubinemia. Further examination of therapeutic potentials of copper chelators in the treatment of severe neonatal hyperbilirubinemia is needed.", publisher = "Srpsko farmakološko društvo Beograd, Novi Sad", journal = "14th Serbian Congress of Farmacologists and 4th Serbian Congress of Clinical Pharmacology with international participation", title = "Penicillamine prevents damaging redox in vitro interactions of bilirubin and copper", pages = "242-240", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2847" }
Bozic Cvijan, B., Korać Jačić, J., Stanković, D., Stanić, M., Popovic Bijelic, A., Bogdanović Pristov, J., Spasojević, I.,& Bajcetic, M.. (2019). Penicillamine prevents damaging redox in vitro interactions of bilirubin and copper. in 14th Serbian Congress of Farmacologists and 4th Serbian Congress of Clinical Pharmacology with international participation Srpsko farmakološko društvo Beograd, Novi Sad., 240-242. https://hdl.handle.net/21.15107/rcub_rimsi_2847
Bozic Cvijan B, Korać Jačić J, Stanković D, Stanić M, Popovic Bijelic A, Bogdanović Pristov J, Spasojević I, Bajcetic M. Penicillamine prevents damaging redox in vitro interactions of bilirubin and copper. in 14th Serbian Congress of Farmacologists and 4th Serbian Congress of Clinical Pharmacology with international participation. 2019;:240-242. https://hdl.handle.net/21.15107/rcub_rimsi_2847 .
Bozic Cvijan, Bojana, Korać Jačić, Jelena, Stanković, Dalibor, Stanić, Marina, Popovic Bijelic, Ana, Bogdanović Pristov, Jelena, Spasojević, Ivan, Bajcetic, Milica, "Penicillamine prevents damaging redox in vitro interactions of bilirubin and copper" in 14th Serbian Congress of Farmacologists and 4th Serbian Congress of Clinical Pharmacology with international participation (2019):240-242, https://hdl.handle.net/21.15107/rcub_rimsi_2847 .