Evaluation of genotoxic pressure along the Sava River
Аутори
Kolarević, StoimirKracun-Kolarevic, Margareta
Kostić, Jovana
Aborgiba, Mustafa
Paunović, Momir
Simonovic, Predrag
Simić, Vladica
Milošković, Aleksandra
Gacic, Zoran
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study we have performed a comprehensive genotoxicological survey along the 900 km of the Sava River - the major drainage basin of the Southeastern Europe and the greatest tributary to the Danube River with 945 km long and 97,713 km large catchment's area extending over Slovenia, Croatia, Bosnia and Herzegovina and Serbia. In total, 12 sites were chosen in compliance with routine monitoring program of the basin member states. The genotoxic potential was assessed by the complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak Alburnus alburnus /Albumoides bipunctatus). We wanted to investigate whether the variation in genotoxic po...tential along the river can be linked to hotspots of faecal and industrial pollution. Hotspots of faecal pollution were identified by bacterial indicators (Escherichia coli numbers) while hotspots related to industry were identified by assessment of concentration of metals in tissue of bleak. The results indicated presence of genotoxic potential along the river resulted from recent or prolonged exposure to genotoxic pressure which can be traced to deterioration of quality of Sava River by urban/industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).
Кључне речи:
Sava River / SOS/umuC / Alburnus alburnus / comet assay / Fpg-modified comet assay / micronucleus testИзвор:
The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic, 2016, 150-Издавач:
- US National Institute of Environmental Health Science, US Environmental Protection Agency
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kolarević, Stoimir AU - Kracun-Kolarevic, Margareta AU - Kostić, Jovana AU - Aborgiba, Mustafa AU - Paunović, Momir AU - Simonovic, Predrag AU - Simić, Vladica AU - Milošković, Aleksandra AU - Gacic, Zoran AU - Vuković-Gačić, Branka PY - 2016 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2764 AB - In this study we have performed a comprehensive genotoxicological survey along the 900 km of the Sava River - the major drainage basin of the Southeastern Europe and the greatest tributary to the Danube River with 945 km long and 97,713 km large catchment's area extending over Slovenia, Croatia, Bosnia and Herzegovina and Serbia. In total, 12 sites were chosen in compliance with routine monitoring program of the basin member states. The genotoxic potential was assessed by the complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak Alburnus alburnus /Albumoides bipunctatus). We wanted to investigate whether the variation in genotoxic potential along the river can be linked to hotspots of faecal and industrial pollution. Hotspots of faecal pollution were identified by bacterial indicators (Escherichia coli numbers) while hotspots related to industry were identified by assessment of concentration of metals in tissue of bleak. The results indicated presence of genotoxic potential along the river resulted from recent or prolonged exposure to genotoxic pressure which can be traced to deterioration of quality of Sava River by urban/industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples). PB - US National Institute of Environmental Health Science, US Environmental Protection Agency C3 - The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic T1 - Evaluation of genotoxic pressure along the Sava River SP - 150 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2764 ER -
@conference{ author = "Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Kostić, Jovana and Aborgiba, Mustafa and Paunović, Momir and Simonovic, Predrag and Simić, Vladica and Milošković, Aleksandra and Gacic, Zoran and Vuković-Gačić, Branka", year = "2016", abstract = "In this study we have performed a comprehensive genotoxicological survey along the 900 km of the Sava River - the major drainage basin of the Southeastern Europe and the greatest tributary to the Danube River with 945 km long and 97,713 km large catchment's area extending over Slovenia, Croatia, Bosnia and Herzegovina and Serbia. In total, 12 sites were chosen in compliance with routine monitoring program of the basin member states. The genotoxic potential was assessed by the complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimurium TA1535/pSK1002. The level of DNA damage as biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak Alburnus alburnus /Albumoides bipunctatus). We wanted to investigate whether the variation in genotoxic potential along the river can be linked to hotspots of faecal and industrial pollution. Hotspots of faecal pollution were identified by bacterial indicators (Escherichia coli numbers) while hotspots related to industry were identified by assessment of concentration of metals in tissue of bleak. The results indicated presence of genotoxic potential along the river resulted from recent or prolonged exposure to genotoxic pressure which can be traced to deterioration of quality of Sava River by urban/industrial wastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).", publisher = "US National Institute of Environmental Health Science, US Environmental Protection Agency", journal = "The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic", title = "Evaluation of genotoxic pressure along the Sava River", pages = "150", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2764" }
Kolarević, S., Kracun-Kolarevic, M., Kostić, J., Aborgiba, M., Paunović, M., Simonovic, P., Simić, V., Milošković, A., Gacic, Z.,& Vuković-Gačić, B.. (2016). Evaluation of genotoxic pressure along the Sava River. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic US National Institute of Environmental Health Science, US Environmental Protection Agency., 150. https://hdl.handle.net/21.15107/rcub_rimsi_2764
Kolarević S, Kracun-Kolarevic M, Kostić J, Aborgiba M, Paunović M, Simonovic P, Simić V, Milošković A, Gacic Z, Vuković-Gačić B. Evaluation of genotoxic pressure along the Sava River. in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic. 2016;:150. https://hdl.handle.net/21.15107/rcub_rimsi_2764 .
Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Kostić, Jovana, Aborgiba, Mustafa, Paunović, Momir, Simonovic, Predrag, Simić, Vladica, Milošković, Aleksandra, Gacic, Zoran, Vuković-Gačić, Branka, "Evaluation of genotoxic pressure along the Sava River" in The Central & Eastern Europe Conference on Health and the Environment (CEECHE), Prague, Czech Republic (2016):150, https://hdl.handle.net/21.15107/rcub_rimsi_2764 .