The European Chub (Squalius cephalus) as an indicator of reservoirs pollution and human health risk assessment associated with its consumption

Abstract

Five reservoirs (Vlasina, Medjuvršje, Zaovine, Perućac, and Garaši) in Serbia were chosen as study sites, which differ by their position, purpose, stages of eutrophication, management policies, and levels of anthropogenic pressure. The objectives of this research were to: determine the concentrations of 26 elements in muscle, gills, and liver of the European chub by inductively-coupled plasma optical emission spectrometry (ICP-OES); determine the concentrations of 17 organochlorine pesticides in fish muscle by gas chromatography with mass spectrometric detection (GC-MS); compare these findings with condition factor (CF) and histopathological (HP) biomarkers; and assess the potential human health risks due to consumption of chub muscle tissue. The highest elemental accumulation was found in the gills. The European chub was not a good indicator of Pb pollution between reservoirs. Concentrations of Hg, As, and Cu were low and did not exceed the proscribed maximum allowed concentrations (MACs). 4,4′-DDE was detected only in individuals from Vlasina, 4,4′-DDD from Perućac and Zaovine, and heptachlor from Zaovine. Low to moderate levels of HP were observed for both gills and liver in all studied reservoirs. HP index for gills was significantly higher for Zaovine compared to Vlasina. Significantly lower HP index for liver and the total HP index value were observed for fish from Vlasina compared to Perućac. No significant human health risks due to the intake of examined pollutants in each reservoir were recorded; women were at higher risk compared to men. A reason for concern is a few muscle samples from Garaši, Vlasina, Perućac, and Medjuvršje in which Cd exceeded the MAC. A reservoir for water supply (Garaši) is generally considered the safest for fish consumption.