Determination of the level and source of microbial pollution in the Sava River Basin
Аутори
Kostić, JovanaKolarević, Stoimir
Kracun-Kolarevic, Margareta
Gacic, Zoran
Aborgiba, Mustafa
Farnleitner, Andreas
Reischer, Georg
Linke, Rita
Paunović, Momir
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction. The contamination of water bodies by sewage or manure is generally determined by fecal indicator bacteria. Specific differentiation between sources of contamination is of particular importance, since the health risk to humans is usually considered higher from human, than from animal fecal contamination. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes fecal markers have gained increased popularity in the field of microbial source tracking (MST).
Aim. The aim of the present study was to assess the level and source of microbial pollution in the Sava River Basin (SRB).
Materials and methods. The sampling at the SRB was performed in September 2015, at 15 locations. Additional samples were collected from 4 wastewater outlets. Quantification of total coliforms, E. coli and enterococci was performed with Colilert Quanti-Tray 2000 and MPN approach. The human-associated BacHum and HF183II, the ruminant-associated BacR and the... pig-associated Pig2Bac fecal markers were enumerated by quantitative PCR (qPCR).
Results. According to the concentrations of fecal indicator bacteria, the river samples were scattered within the classes I and II (slight to moderate pollution). According to all monitored indicators, samples collected at 3 wastewater outlets were excessively contaminated. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II at 13 locations. The ruminant-associated BacR and the pig-associated Pig-2-Bac markers were not detected. High correlation was observed between the standard fecal indicators and human associated fecal markers.
Conclusions. The results reveal human origin of fecal pollution in the SRB and indicates the urgent need for effective wastewater treatment plants in water management.
Acknowledgements. EU Seventh Framework Programme – GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, and the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.
Кључне речи:
microbial source tracking / faecal indicators / Sava River BasinИзвор:
10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria, 2017, 379-Издавач:
- Balkan Society for Microbiology
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kostić, Jovana AU - Kolarević, Stoimir AU - Kracun-Kolarevic, Margareta AU - Gacic, Zoran AU - Aborgiba, Mustafa AU - Farnleitner, Andreas AU - Reischer, Georg AU - Linke, Rita AU - Paunović, Momir AU - Vuković-Gačić, Branka PY - 2017 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2654 AB - Introduction. The contamination of water bodies by sewage or manure is generally determined by fecal indicator bacteria. Specific differentiation between sources of contamination is of particular importance, since the health risk to humans is usually considered higher from human, than from animal fecal contamination. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes fecal markers have gained increased popularity in the field of microbial source tracking (MST). Aim. The aim of the present study was to assess the level and source of microbial pollution in the Sava River Basin (SRB). Materials and methods. The sampling at the SRB was performed in September 2015, at 15 locations. Additional samples were collected from 4 wastewater outlets. Quantification of total coliforms, E. coli and enterococci was performed with Colilert Quanti-Tray 2000 and MPN approach. The human-associated BacHum and HF183II, the ruminant-associated BacR and the pig-associated Pig2Bac fecal markers were enumerated by quantitative PCR (qPCR). Results. According to the concentrations of fecal indicator bacteria, the river samples were scattered within the classes I and II (slight to moderate pollution). According to all monitored indicators, samples collected at 3 wastewater outlets were excessively contaminated. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II at 13 locations. The ruminant-associated BacR and the pig-associated Pig-2-Bac markers were not detected. High correlation was observed between the standard fecal indicators and human associated fecal markers. Conclusions. The results reveal human origin of fecal pollution in the SRB and indicates the urgent need for effective wastewater treatment plants in water management. Acknowledgements. EU Seventh Framework Programme – GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, and the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33. PB - Balkan Society for Microbiology C3 - 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria T1 - Determination of the level and source of microbial pollution in the Sava River Basin SP - 379 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2654 ER -
@conference{ author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Gacic, Zoran and Aborgiba, Mustafa and Farnleitner, Andreas and Reischer, Georg and Linke, Rita and Paunović, Momir and Vuković-Gačić, Branka", year = "2017", abstract = "Introduction. The contamination of water bodies by sewage or manure is generally determined by fecal indicator bacteria. Specific differentiation between sources of contamination is of particular importance, since the health risk to humans is usually considered higher from human, than from animal fecal contamination. Quantitative PCR (qPCR)-based assays for analysis of human- or animal-associated genetic Bacteroidetes fecal markers have gained increased popularity in the field of microbial source tracking (MST). Aim. The aim of the present study was to assess the level and source of microbial pollution in the Sava River Basin (SRB). Materials and methods. The sampling at the SRB was performed in September 2015, at 15 locations. Additional samples were collected from 4 wastewater outlets. Quantification of total coliforms, E. coli and enterococci was performed with Colilert Quanti-Tray 2000 and MPN approach. The human-associated BacHum and HF183II, the ruminant-associated BacR and the pig-associated Pig2Bac fecal markers were enumerated by quantitative PCR (qPCR). Results. According to the concentrations of fecal indicator bacteria, the river samples were scattered within the classes I and II (slight to moderate pollution). According to all monitored indicators, samples collected at 3 wastewater outlets were excessively contaminated. The results of MST revealed the presence of human-associated fecal markers BacHum and HF183II at 13 locations. The ruminant-associated BacR and the pig-associated Pig-2-Bac markers were not detected. High correlation was observed between the standard fecal indicators and human associated fecal markers. Conclusions. The results reveal human origin of fecal pollution in the SRB and indicates the urgent need for effective wastewater treatment plants in water management. Acknowledgements. EU Seventh Framework Programme – GLOBAQUA (no. 603629-ENV-2013-6.2.1). Ministry of Education, Science and Technological Development of the Republic of Serbia projects no. 173045 and 173025, and the bilateral project Serbia and Austria: SER Ev. No. 451-03-01039/2015-09/33.", publisher = "Balkan Society for Microbiology", journal = "10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria", title = "Determination of the level and source of microbial pollution in the Sava River Basin", pages = "379", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2654" }
Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Gacic, Z., Aborgiba, M., Farnleitner, A., Reischer, G., Linke, R., Paunović, M.,& Vuković-Gačić, B.. (2017). Determination of the level and source of microbial pollution in the Sava River Basin. in 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria Balkan Society for Microbiology., 379. https://hdl.handle.net/21.15107/rcub_rimsi_2654
Kostić J, Kolarević S, Kracun-Kolarevic M, Gacic Z, Aborgiba M, Farnleitner A, Reischer G, Linke R, Paunović M, Vuković-Gačić B. Determination of the level and source of microbial pollution in the Sava River Basin. in 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria. 2017;:379. https://hdl.handle.net/21.15107/rcub_rimsi_2654 .
Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Gacic, Zoran, Aborgiba, Mustafa, Farnleitner, Andreas, Reischer, Georg, Linke, Rita, Paunović, Momir, Vuković-Gačić, Branka, "Determination of the level and source of microbial pollution in the Sava River Basin" in 10th Balkan Congress of Microbiology, Microbiologia Balkanica’2017, Sofia , Bulgaria (2017):379, https://hdl.handle.net/21.15107/rcub_rimsi_2654 .