Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish
Аутори
Kostić, JovanaKolarević, Stoimir
Kracun-Kolarevic, Margareta
Aborgiba, Mustafa
Gacic, Zoran
Lenhardt, Mirjana
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A number of biological consequences of DNA damage can be initiated at the cellular, organ, whole animal, population and community levels. The comet assay, single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for detection of DNA damage in individual cells based on the migration of denatured DNA during electrophoresis, in which damaged nuclei form comet-like shapes. Since, the large rivers are constantly exposed to variety of chemicals we tested genotoxic potential of rivers Sava and Danube in the Belgrade region, on cyprinid fish. DNA damage was monitored in blood, liver and gill cells of Blicca bjoerkna, Ballerus sapa and Abramis brama by comet assay. The specimens of B. bjoerkna and B. sapa were sampled at the site Duboko (Sava river), which is exposed to untreated municipal wastewater, effluents of the Thermal Power Plant and intensive agricultural activity. The specimens of A. brama were sampled at the site Višnjica (Danube river), which is exposed to untreate...d municipal wastewater from surrounding Belgrade municipalities. Sampling was performed during February and August 2014. Comet IV Computer Software (Perceptive Instruments, UK) was used for the quantification of DNA damage. The increased level of DNA damage obtained in all tissues of B. bjoerkna and B. sapa (Sava river), and A. brama (Danube river) is in correlation with high chemical pollution detected at Duboko and Višnjica sites. Moreover, this study showed the influence of seasonal variations on the level of DNA damage.
Кључне речи:
genotoxicity / comet assay / Cyprinid fishИзвор:
III Simpozijum biologa i ekologa Republike Srpske, 2015, 203-Издавач:
- Природно-математички факултет, Бања Лука
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kostić, Jovana AU - Kolarević, Stoimir AU - Kracun-Kolarevic, Margareta AU - Aborgiba, Mustafa AU - Gacic, Zoran AU - Lenhardt, Mirjana AU - Vuković-Gačić, Branka PY - 2015 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2652 AB - A number of biological consequences of DNA damage can be initiated at the cellular, organ, whole animal, population and community levels. The comet assay, single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for detection of DNA damage in individual cells based on the migration of denatured DNA during electrophoresis, in which damaged nuclei form comet-like shapes. Since, the large rivers are constantly exposed to variety of chemicals we tested genotoxic potential of rivers Sava and Danube in the Belgrade region, on cyprinid fish. DNA damage was monitored in blood, liver and gill cells of Blicca bjoerkna, Ballerus sapa and Abramis brama by comet assay. The specimens of B. bjoerkna and B. sapa were sampled at the site Duboko (Sava river), which is exposed to untreated municipal wastewater, effluents of the Thermal Power Plant and intensive agricultural activity. The specimens of A. brama were sampled at the site Višnjica (Danube river), which is exposed to untreated municipal wastewater from surrounding Belgrade municipalities. Sampling was performed during February and August 2014. Comet IV Computer Software (Perceptive Instruments, UK) was used for the quantification of DNA damage. The increased level of DNA damage obtained in all tissues of B. bjoerkna and B. sapa (Sava river), and A. brama (Danube river) is in correlation with high chemical pollution detected at Duboko and Višnjica sites. Moreover, this study showed the influence of seasonal variations on the level of DNA damage. PB - Природно-математички факултет, Бања Лука C3 - III Simpozijum biologa i ekologa Republike Srpske T1 - Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish SP - 203 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2652 ER -
@conference{ author = "Kostić, Jovana and Kolarević, Stoimir and Kracun-Kolarevic, Margareta and Aborgiba, Mustafa and Gacic, Zoran and Lenhardt, Mirjana and Vuković-Gačić, Branka", year = "2015", abstract = "A number of biological consequences of DNA damage can be initiated at the cellular, organ, whole animal, population and community levels. The comet assay, single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for detection of DNA damage in individual cells based on the migration of denatured DNA during electrophoresis, in which damaged nuclei form comet-like shapes. Since, the large rivers are constantly exposed to variety of chemicals we tested genotoxic potential of rivers Sava and Danube in the Belgrade region, on cyprinid fish. DNA damage was monitored in blood, liver and gill cells of Blicca bjoerkna, Ballerus sapa and Abramis brama by comet assay. The specimens of B. bjoerkna and B. sapa were sampled at the site Duboko (Sava river), which is exposed to untreated municipal wastewater, effluents of the Thermal Power Plant and intensive agricultural activity. The specimens of A. brama were sampled at the site Višnjica (Danube river), which is exposed to untreated municipal wastewater from surrounding Belgrade municipalities. Sampling was performed during February and August 2014. Comet IV Computer Software (Perceptive Instruments, UK) was used for the quantification of DNA damage. The increased level of DNA damage obtained in all tissues of B. bjoerkna and B. sapa (Sava river), and A. brama (Danube river) is in correlation with high chemical pollution detected at Duboko and Višnjica sites. Moreover, this study showed the influence of seasonal variations on the level of DNA damage.", publisher = "Природно-математички факултет, Бања Лука", journal = "III Simpozijum biologa i ekologa Republike Srpske", title = "Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish", pages = "203", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2652" }
Kostić, J., Kolarević, S., Kracun-Kolarevic, M., Aborgiba, M., Gacic, Z., Lenhardt, M.,& Vuković-Gačić, B.. (2015). Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish. in III Simpozijum biologa i ekologa Republike Srpske Природно-математички факултет, Бања Лука., 203. https://hdl.handle.net/21.15107/rcub_rimsi_2652
Kostić J, Kolarević S, Kracun-Kolarevic M, Aborgiba M, Gacic Z, Lenhardt M, Vuković-Gačić B. Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish. in III Simpozijum biologa i ekologa Republike Srpske. 2015;:203. https://hdl.handle.net/21.15107/rcub_rimsi_2652 .
Kostić, Jovana, Kolarević, Stoimir, Kracun-Kolarevic, Margareta, Aborgiba, Mustafa, Gacic, Zoran, Lenhardt, Mirjana, Vuković-Gačić, Branka, "Evaluation of the Sava and the Danube river genotoxicity by Comet assay on different cyprinid fish" in III Simpozijum biologa i ekologa Republike Srpske (2015):203, https://hdl.handle.net/21.15107/rcub_rimsi_2652 .