Приказ основних података о документу

dc.creatorHadzi-Tasković Sukalović, Vesna
dc.creatorVuletic, Mirjana
dc.creatorVučinić, Željko
dc.creatorVeljović-Jovanović, Sonja
dc.date.accessioned2022-04-05T14:16:01Z
dc.date.available2022-04-05T14:16:01Z
dc.date.issued2008
dc.identifier.issn0918-9440
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/264
dc.description.abstractThe rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.en
dc.publisherSpringer Japan Kk, Tokyo
dc.rightsrestrictedAccess
dc.sourceJournal of Plant Research
dc.subjectphenoxyl radicalsen
dc.subjectperoxidaseen
dc.subjectNADHen
dc.subjecthydroxycinnamatesen
dc.subjectascorbateen
dc.titleEffectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADHen
dc.typearticle
dc.rights.licenseARR
dc.citation.epage123
dc.citation.issue1
dc.citation.other121(1): 115-123
dc.citation.rankM22
dc.citation.spage115
dc.citation.volume121
dc.identifier.doi10.1007/s10265-007-0124-x
dc.identifier.pmid18071845
dc.identifier.scopus2-s2.0-38049001193
dc.identifier.wos000252192500014
dc.type.versionpublishedVersion


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Приказ основних података о документу