Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents
Само за регистроване кориснике
2023
Аутори
Milojkov, DušanRadosavljević-Mihajlović, Ana S
Stanić, Vojislav Dj
Nastasijević, Branislav
Radotić, Ksenija
Janković Častvan, Ivona
Živković-Radovanović, Vukosava
Чланак у часопису (Објављена верзија)
,
Elsevier
Метаподаци
Приказ свих података о документуАпстракт
Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential
next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized
by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible
properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate
the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images
confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis
shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure.
Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal
lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence
in violet–blue col...or. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and
Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic
action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample
in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three
species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium
growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of
Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F�����
ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples
were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad–
spectrum antimicrobial agents for various biomedical and health care preparations.
Кључне речи:
Antimicrobial agent / Fluorapatite / Nanocrystals / Cu2+ ions / Neutralization method / Synergy effectИзвор:
Journal of Photochemistry & Photobiology, B: Biology, 2023, 239, 112649-Издавач:
- Elsevier
Финансирање / пројекти:
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Milojkov, Dušan AU - Radosavljević-Mihajlović, Ana S AU - Stanić, Vojislav Dj AU - Nastasijević, Branislav AU - Radotić, Ksenija AU - Janković Častvan, Ivona AU - Živković-Radovanović, Vukosava PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2649 AB - Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F����� ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad– spectrum antimicrobial agents for various biomedical and health care preparations. PB - Elsevier T2 - Journal of Photochemistry & Photobiology, B: Biology T1 - Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents SP - 112649 VL - 239 DO - 10.1016/j.jphotobiol.2023.112649 ER -
@article{ author = "Milojkov, Dušan and Radosavljević-Mihajlović, Ana S and Stanić, Vojislav Dj and Nastasijević, Branislav and Radotić, Ksenija and Janković Častvan, Ivona and Živković-Radovanović, Vukosava", year = "2023", abstract = "Nanomaterials based on metal–doped fluorapatite (FAP) have attracted considerable interest as potential next–generation antimicrobial agents. In this study, Cu2+–doped FAP nanocrystals have been successfully synthesized by a neutralization method at room temperature. Their structural, optical, antimicrobial, and hemcompatible properties have been investigated. XRD, FTIR, FESEM, and N2 adsorption–desorption studies indicate the formation of single–phase FAP mesoporous nanopowders, composed of rod–like particles. TEM images confirmed the formation of nanorodes with a length of 60 nm and a width of about 18 nm. Rietveld analysis shows that the Cu2+ ions preferentially substitute Ca2 (6 h) sites in the hexagonal fluorapatite crystal structure. Fluorescence spectroscopy accompanied by MCR–ALS method confirms substitution of Cu2+ ions in FAP crystal lattice with extracting additional d–d band transition at green color from FAP broadband self–activated luminescence in violet–blue color. Antimicrobial studies conducted on Staphylococcus aureus, Escherichia coli and Micrococcus lysodeikticus showed that FAP nanopowder with the highest Cu2+ content have strong bacteriostatic action on Staphylococcus aureus bacterial strain in mediums containing nutrition matters. In addition, this sample in comparison to pure FAP achieved a high percentage of relative reduction of bacterial population for all three species, being >90% in most cases. Fungistatic action is noticed too, throwgh the slowing down mycelium growth of fungus Aspergillus niger, Aspergillus flavus and Penicillium roqueforti and reduction of sporulation of Aspergillus niger species. Cu2+–doped FAP nanocrystals shows a synergistic antimicrobial effect with Cu2+ and F����� ions. Concerning the potential biomedical applications, the hemolysis ratios of the Cu2+–doped FAP samples were below 5%. The obtained results pointed out the possible use of the synthesized nanocrystals as broad– spectrum antimicrobial agents for various biomedical and health care preparations.", publisher = "Elsevier", journal = "Journal of Photochemistry & Photobiology, B: Biology", title = "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents", pages = "112649", volume = "239", doi = "10.1016/j.jphotobiol.2023.112649" }
Milojkov, D., Radosavljević-Mihajlović, A. S., Stanić, V. D., Nastasijević, B., Radotić, K., Janković Častvan, I.,& Živković-Radovanović, V.. (2023). Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry & Photobiology, B: Biology Elsevier., 239, 112649. https://doi.org/10.1016/j.jphotobiol.2023.112649
Milojkov D, Radosavljević-Mihajlović AS, Stanić VD, Nastasijević B, Radotić K, Janković Častvan I, Živković-Radovanović V. Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents. in Journal of Photochemistry & Photobiology, B: Biology. 2023;239:112649. doi:10.1016/j.jphotobiol.2023.112649 .
Milojkov, Dušan, Radosavljević-Mihajlović, Ana S, Stanić, Vojislav Dj, Nastasijević, Branislav, Radotić, Ksenija, Janković Častvan, Ivona, Živković-Radovanović, Vukosava, "Synthesis and characterization of luminescent Cu2+–doped fluorapatite nanocrystals as potential broad–spectrum antimicrobial agents" in Journal of Photochemistry & Photobiology, B: Biology, 239 (2023):112649, https://doi.org/10.1016/j.jphotobiol.2023.112649 . .