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Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH

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2008
Authors
Hadzi-Tasković Sukalović, Vesna
Vuletic, Mirjana
Vučinić, Željko
Veljović-Jovanović, Sonja
Article (Published version)
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Abstract
The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation..., with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.

Keywords:
phenoxyl radicals / peroxidase / NADH / hydroxycinnamates / ascorbate
Source:
Journal of Plant Research, 2008, 121, 1, 115-123
Publisher:
  • Springer Japan Kk, Tokyo

DOI: 10.1007/s10265-007-0124-x

ISSN: 0918-9440

PubMed: 18071845

WoS: 000252192500014

Scopus: 2-s2.0-38049001193
[ Google Scholar ]
10
8
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/264
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - JOUR
AU  - Hadzi-Tasković Sukalović, Vesna
AU  - Vuletic, Mirjana
AU  - Vučinić, Željko
AU  - Veljović-Jovanović, Sonja
PY  - 2008
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/264
AB  - The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.
PB  - Springer Japan Kk, Tokyo
T2  - Journal of Plant Research
T1  - Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH
EP  - 123
IS  - 1
SP  - 115
VL  - 121
DO  - 10.1007/s10265-007-0124-x
ER  - 
@article{
author = "Hadzi-Tasković Sukalović, Vesna and Vuletic, Mirjana and Vučinić, Željko and Veljović-Jovanović, Sonja",
year = "2008",
abstract = "The rate of ascorbate and nicotinamide adenine dinucleotide plus hydrogen (NADH) cooxidation (i.e., their nonenzymic oxidation by peroxidase/H2O2-generated phenoxyl radicals of three hydroxycinnamates: caffeate, ferulate and p-coumarate) was studied in vitro. The reactions initiated by different sources of peroxidase (EC 1.11.1.7) [isolates from soybean (Glycine max L.) seed coat, maize (Zea mays L.) root-cell wall, and commercial horseradish peroxidase] were monitored. Native electrophoresis of samples and specific staining for peroxidase activity revealed various isoforms in each of the three enzyme sources. The peroxidase sources differed both in the rate of H2O2-dependent hydroxycinnamate oxidation and in the order of affinity for the phenolic substrates. The three hydroxycinnamates did not differ in their ability to cooxidize ascorbate, whereas NADH cooxidation was affected by substitution of the phenolic ring. Thus, p-coumarate was more efficient than caffeate in NADH cooxidation, with ferulate not being effective at all. Metal ions (Zn2+ and Al3+) inhibited the reaction of peroxidase with p-coumarate and affected the cooxidation rate of ascorbate and the peroxidase reaction in the same manner with all substrates used. However, inhibition of p-coumarate oxidation by metal ions did not affect NADH cooxidation rate. We propose that both the ascorbate and NADH cooxidation systems can function as mechanisms to scavenge H2O2 and regenerate phenolics in different cellular compartments, thus contributing to protection from oxidative damage.",
publisher = "Springer Japan Kk, Tokyo",
journal = "Journal of Plant Research",
title = "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH",
pages = "123-115",
number = "1",
volume = "121",
doi = "10.1007/s10265-007-0124-x"
}
Hadzi-Tasković Sukalović, V., Vuletic, M., Vučinić, Ž.,& Veljović-Jovanović, S.. (2008). Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research
Springer Japan Kk, Tokyo., 121(1), 115-123.
https://doi.org/10.1007/s10265-007-0124-x
Hadzi-Tasković Sukalović V, Vuletic M, Vučinić Ž, Veljović-Jovanović S. Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH. in Journal of Plant Research. 2008;121(1):115-123.
doi:10.1007/s10265-007-0124-x .
Hadzi-Tasković Sukalović, Vesna, Vuletic, Mirjana, Vučinić, Željko, Veljović-Jovanović, Sonja, "Effectiveness of phenoxyl radicals generated by peroxidase/H2O2-catalyzed oxidation of caffeate, ferulate, and p-coumarate in cooxidation of ascorbate and NADH" in Journal of Plant Research, 121, no. 1 (2008):115-123,
https://doi.org/10.1007/s10265-007-0124-x . .

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