Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study
Само за регистроване кориснике
2008
Аутори
Harhaji, Lj.Mijatović, Sanja
Maksimović-Ivanić, Danijela
Stojanović, I.
Momcilović, Miljana
Maksimović, Vuk
Tufegdžić, Srđan J.
Marjanović, Žaklina
Mostarica-Stojković, M.
Vučinić, Željko
Stosic-Grujicic, Stanislava
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages ...collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.
Кључне речи:
tumor apoptosis / methanol extract / Coriolus versicolor / cell cycle / B16 melanoma / anti-tumor macrophagesИзвор:
Food and Chemical Toxicology, 2008, 46, 5, 1825-1833Издавач:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/j.fct.2008.01.027
ISSN: 0278-6915
PubMed: 18313195
WoS: 000255328400048
Scopus: 2-s2.0-41049100813
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Harhaji, Lj. AU - Mijatović, Sanja AU - Maksimović-Ivanić, Danijela AU - Stojanović, I. AU - Momcilović, Miljana AU - Maksimović, Vuk AU - Tufegdžić, Srđan J. AU - Marjanović, Žaklina AU - Mostarica-Stojković, M. AU - Vučinić, Željko AU - Stosic-Grujicic, Stanislava PY - 2008 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/262 AB - Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Food and Chemical Toxicology T1 - Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study EP - 1833 IS - 5 SP - 1825 VL - 46 DO - 10.1016/j.fct.2008.01.027 ER -
@article{ author = "Harhaji, Lj. and Mijatović, Sanja and Maksimović-Ivanić, Danijela and Stojanović, I. and Momcilović, Miljana and Maksimović, Vuk and Tufegdžić, Srđan J. and Marjanović, Žaklina and Mostarica-Stojković, M. and Vučinić, Željko and Stosic-Grujicic, Stanislava", year = "2008", abstract = "Numerous studies have shown immunostimulatory and anti-tumor effects of water and standardized aqueous ethanol extracts derived from the medicinal mushroom, Coriolus versicolor, but the biological activity of methanol extracts has not been examined so far. In the present study we investigated the anti-tumor effect of C versicolor methanol extract (which contains terpenoids and poly-phenols) on B16 mouse melanoma cells both in vitro and in vivo. In vitro treatment of the cells with the methanol extract (25-1600 mu g/ml) reduced melanoma cell viability in it dose-dependent manner. Furthermore, in the presence of the methanol extract (200 mu g/ml, concentration IC50) the proliferation of B16 cells was arrested in the G(0)/G(1) phase of the cell cycle, followed by both apoptotic and secondary necrotic cell death. In vivo methanol extract treatment (i.p. 50 mg/kg, for 14 days) inhibited tumor growth in C57BL/6 mice inoculated with syngeneic B16 tumor cells. Moreover, peritoneal macrophages collected 21 days after tumor implantation from methanol extract-treated animals exerted stronger tumoristatic activity ex vivo than macrophages from control melanoma-bearing rnice. Taken together, our results demonstrate that C. versicolor methanol extract exerts pronounced anti-melanoma activity, both directly through antiproliferative and cytotoxic effects on tumor cells and indirectly through promotion of macrophage anti-tumor activity.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Food and Chemical Toxicology", title = "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study", pages = "1833-1825", number = "5", volume = "46", doi = "10.1016/j.fct.2008.01.027" }
Harhaji, Lj., Mijatović, S., Maksimović-Ivanić, D., Stojanović, I., Momcilović, M., Maksimović, V., Tufegdžić, S. J., Marjanović, Ž., Mostarica-Stojković, M., Vučinić, Ž.,& Stosic-Grujicic, S.. (2008). Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology Pergamon-Elsevier Science Ltd, Oxford., 46(5), 1825-1833. https://doi.org/10.1016/j.fct.2008.01.027
Harhaji L, Mijatović S, Maksimović-Ivanić D, Stojanović I, Momcilović M, Maksimović V, Tufegdžić SJ, Marjanović Ž, Mostarica-Stojković M, Vučinić Ž, Stosic-Grujicic S. Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study. in Food and Chemical Toxicology. 2008;46(5):1825-1833. doi:10.1016/j.fct.2008.01.027 .
Harhaji, Lj., Mijatović, Sanja, Maksimović-Ivanić, Danijela, Stojanović, I., Momcilović, Miljana, Maksimović, Vuk, Tufegdžić, Srđan J., Marjanović, Žaklina, Mostarica-Stojković, M., Vučinić, Željko, Stosic-Grujicic, Stanislava, "Anti-tumor effect of Coriolus versicolor methanol extract against mouse B16 melanoma cells: In vitro and in vivo study" in Food and Chemical Toxicology, 46, no. 5 (2008):1825-1833, https://doi.org/10.1016/j.fct.2008.01.027 . .