Labeling of the isolated plant cell walls with CdSe Quantum dots
Abstract
Quantum dots (QDs) are semiconductor nanoparticles with
increasing application as fluorescent markers in biology.We
investigated structure of the cell walls of different species
complexed with CdSe QDs using fluorescence microscopy,
fluorescence spectroscopy and FTIR techniques. In the experiments we used the cell walls isolated from three distinct plant species: Arabidopsis thaliana, Acer sp. and Picea
omorika. We studied both unlabeled and CdSe-labeled cell
walls. Fluorescence spectroscopy and microscopy were used
for detection of QDs alone or complexed to the cell walls.
Emission spectra were deconvolved using the Nelder–Mead
algorithm in Matlab 6.5. We calculated approximate probability distribution (APD) for positions of spectral component
maxima. There was certain difference between unlabeled cell
walls and those complexed with QDs. The FTIR spectra also
show some difference between the complexed and pure cell
walls. The results show that structure was changed, but n...ot
significantly in reaction with CdSe QDs. These results are
promising in context of use of QDs as labels in cell wall studies. The characterization of the complex of cell wall structure
with QDs is a part of the study of nanoparticles application
in investigations of plant materials.
Keywords:
quantum dots / cell wall / CdSe / fluorescence spectroscopy / fluorescence microscopySource:
7th European Biophysics Congress, 2009, 38Publisher:
- Europian Biophysical Societies Association
Funding / projects:
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-MESTD-Basic Research (BR or ON)-173017)
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Đikanović, Daniela AU - Kalauzi, Aleksandar AU - Drakulic, Branko AU - Vannoy, Charles AU - Radotić, Ksenija PY - 2009 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2594 AB - Quantum dots (QDs) are semiconductor nanoparticles with increasing application as fluorescent markers in biology.We investigated structure of the cell walls of different species complexed with CdSe QDs using fluorescence microscopy, fluorescence spectroscopy and FTIR techniques. In the experiments we used the cell walls isolated from three distinct plant species: Arabidopsis thaliana, Acer sp. and Picea omorika. We studied both unlabeled and CdSe-labeled cell walls. Fluorescence spectroscopy and microscopy were used for detection of QDs alone or complexed to the cell walls. Emission spectra were deconvolved using the Nelder–Mead algorithm in Matlab 6.5. We calculated approximate probability distribution (APD) for positions of spectral component maxima. There was certain difference between unlabeled cell walls and those complexed with QDs. The FTIR spectra also show some difference between the complexed and pure cell walls. The results show that structure was changed, but not significantly in reaction with CdSe QDs. These results are promising in context of use of QDs as labels in cell wall studies. The characterization of the complex of cell wall structure with QDs is a part of the study of nanoparticles application in investigations of plant materials. PB - Europian Biophysical Societies Association C3 - 7th European Biophysics Congress T1 - Labeling of the isolated plant cell walls with CdSe Quantum dots IS - 38 DO - 10.1007/s00249-009-0478-1 ER -
@conference{ author = "Đikanović, Daniela and Kalauzi, Aleksandar and Drakulic, Branko and Vannoy, Charles and Radotić, Ksenija", year = "2009", abstract = "Quantum dots (QDs) are semiconductor nanoparticles with increasing application as fluorescent markers in biology.We investigated structure of the cell walls of different species complexed with CdSe QDs using fluorescence microscopy, fluorescence spectroscopy and FTIR techniques. In the experiments we used the cell walls isolated from three distinct plant species: Arabidopsis thaliana, Acer sp. and Picea omorika. We studied both unlabeled and CdSe-labeled cell walls. Fluorescence spectroscopy and microscopy were used for detection of QDs alone or complexed to the cell walls. Emission spectra were deconvolved using the Nelder–Mead algorithm in Matlab 6.5. We calculated approximate probability distribution (APD) for positions of spectral component maxima. There was certain difference between unlabeled cell walls and those complexed with QDs. The FTIR spectra also show some difference between the complexed and pure cell walls. The results show that structure was changed, but not significantly in reaction with CdSe QDs. These results are promising in context of use of QDs as labels in cell wall studies. The characterization of the complex of cell wall structure with QDs is a part of the study of nanoparticles application in investigations of plant materials.", publisher = "Europian Biophysical Societies Association", journal = "7th European Biophysics Congress", title = "Labeling of the isolated plant cell walls with CdSe Quantum dots", number = "38", doi = "10.1007/s00249-009-0478-1" }
Đikanović, D., Kalauzi, A., Drakulic, B., Vannoy, C.,& Radotić, K.. (2009). Labeling of the isolated plant cell walls with CdSe Quantum dots. in 7th European Biophysics Congress Europian Biophysical Societies Association.(38). https://doi.org/10.1007/s00249-009-0478-1
Đikanović D, Kalauzi A, Drakulic B, Vannoy C, Radotić K. Labeling of the isolated plant cell walls with CdSe Quantum dots. in 7th European Biophysics Congress. 2009;(38). doi:10.1007/s00249-009-0478-1 .
Đikanović, Daniela, Kalauzi, Aleksandar, Drakulic, Branko, Vannoy, Charles, Radotić, Ksenija, "Labeling of the isolated plant cell walls with CdSe Quantum dots" in 7th European Biophysics Congress, no. 38 (2009), https://doi.org/10.1007/s00249-009-0478-1 . .