Detection of genotoxicity in situ in freshwater worm Branchiura sowerbyi Beddard, 1982 (Oligocheta: Tubificidae)
Аутори
Kracun-Kolarevic, MargaretaKolarević, Stoimir
Atanacković, Ana
Kostić, Jovana
Gacic, Zoran
Paunović, Momir
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This study is the first report of application of comet assay for in situ detection of genotoxicity on Branchiura sowerbyi, a freshwater worm commonly found in organic-rich sediment. This species has detritivorous way of feeding, sediment burrowing way of life characterized by low mobility which makes these organisms easily affected by the presence of xenobiotics in aquatic environment. Sampling was done monthly during 2014 on two sites, one on the Sava River (Duboko) and one on the Danube River (Višnjica). Images of 200 nuclei for each sampling were analyzed with a fluorescence microscope and scored with software (Comet Assay IV). Specimens from the site Duboko were characterized by higher DNA damage in comparison with Višnjica. Significant correlation was observed with DNA damage in specimens of fish and mussels collected in parallel at the selected sites. Physical, chemical parameters and microbiological quality of water assessed each month are in good correlation with comet assay. A...ccording to our results, B. sowerbyi could be used for in situ detection of genotoxicity.
Кључне речи:
comet assay / Branchiura sowerbyi / genotoxicity / the Danube River / the Sava RiverИзвор:
V Congress of the Serbian Genetic Society, 2014, 134-Издавач:
- Serbian Genetic Society, Belgrade, Serbia
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kracun-Kolarevic, Margareta AU - Kolarević, Stoimir AU - Atanacković, Ana AU - Kostić, Jovana AU - Gacic, Zoran AU - Paunović, Momir AU - Vuković-Gačić, Branka PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2512 AB - This study is the first report of application of comet assay for in situ detection of genotoxicity on Branchiura sowerbyi, a freshwater worm commonly found in organic-rich sediment. This species has detritivorous way of feeding, sediment burrowing way of life characterized by low mobility which makes these organisms easily affected by the presence of xenobiotics in aquatic environment. Sampling was done monthly during 2014 on two sites, one on the Sava River (Duboko) and one on the Danube River (Višnjica). Images of 200 nuclei for each sampling were analyzed with a fluorescence microscope and scored with software (Comet Assay IV). Specimens from the site Duboko were characterized by higher DNA damage in comparison with Višnjica. Significant correlation was observed with DNA damage in specimens of fish and mussels collected in parallel at the selected sites. Physical, chemical parameters and microbiological quality of water assessed each month are in good correlation with comet assay. According to our results, B. sowerbyi could be used for in situ detection of genotoxicity. PB - Serbian Genetic Society, Belgrade, Serbia C3 - V Congress of the Serbian Genetic Society T1 - Detection of genotoxicity in situ in freshwater worm Branchiura sowerbyi Beddard, 1982 (Oligocheta: Tubificidae) SP - 134 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2512 ER -
@conference{ author = "Kracun-Kolarevic, Margareta and Kolarević, Stoimir and Atanacković, Ana and Kostić, Jovana and Gacic, Zoran and Paunović, Momir and Vuković-Gačić, Branka", year = "2014", abstract = "This study is the first report of application of comet assay for in situ detection of genotoxicity on Branchiura sowerbyi, a freshwater worm commonly found in organic-rich sediment. This species has detritivorous way of feeding, sediment burrowing way of life characterized by low mobility which makes these organisms easily affected by the presence of xenobiotics in aquatic environment. Sampling was done monthly during 2014 on two sites, one on the Sava River (Duboko) and one on the Danube River (Višnjica). Images of 200 nuclei for each sampling were analyzed with a fluorescence microscope and scored with software (Comet Assay IV). Specimens from the site Duboko were characterized by higher DNA damage in comparison with Višnjica. Significant correlation was observed with DNA damage in specimens of fish and mussels collected in parallel at the selected sites. Physical, chemical parameters and microbiological quality of water assessed each month are in good correlation with comet assay. According to our results, B. sowerbyi could be used for in situ detection of genotoxicity.", publisher = "Serbian Genetic Society, Belgrade, Serbia", journal = "V Congress of the Serbian Genetic Society", title = "Detection of genotoxicity in situ in freshwater worm Branchiura sowerbyi Beddard, 1982 (Oligocheta: Tubificidae)", pages = "134", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2512" }
Kracun-Kolarevic, M., Kolarević, S., Atanacković, A., Kostić, J., Gacic, Z., Paunović, M.,& Vuković-Gačić, B.. (2014). Detection of genotoxicity in situ in freshwater worm Branchiura sowerbyi Beddard, 1982 (Oligocheta: Tubificidae). in V Congress of the Serbian Genetic Society Serbian Genetic Society, Belgrade, Serbia., 134. https://hdl.handle.net/21.15107/rcub_rimsi_2512
Kracun-Kolarevic M, Kolarević S, Atanacković A, Kostić J, Gacic Z, Paunović M, Vuković-Gačić B. Detection of genotoxicity in situ in freshwater worm Branchiura sowerbyi Beddard, 1982 (Oligocheta: Tubificidae). in V Congress of the Serbian Genetic Society. 2014;:134. https://hdl.handle.net/21.15107/rcub_rimsi_2512 .
Kracun-Kolarevic, Margareta, Kolarević, Stoimir, Atanacković, Ana, Kostić, Jovana, Gacic, Zoran, Paunović, Momir, Vuković-Gačić, Branka, "Detection of genotoxicity in situ in freshwater worm Branchiura sowerbyi Beddard, 1982 (Oligocheta: Tubificidae)" in V Congress of the Serbian Genetic Society (2014):134, https://hdl.handle.net/21.15107/rcub_rimsi_2512 .