Impact of in vivo and in vitro exposure to selected cytostatics on DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus
Аутори
Kolarević, StoimirGacic, Zoran
Kostić, Jovana
Sunjog, Karolina
Kracun-Kolarevic, Margareta
Paunović, Momir
Knežević-Vukčević, Jelena
Vuković-Gačić, Branka
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The impact of 5-Fluorouracil (5-FU), Cisplatin (CP) and Etoposide (Eto) on DNA damage level was studied in vivo and in vitro on haemocytes of freshwater mussels Unio pictorum and U. tumidus using alkaline comet assay. For in vitro treatment, two different approaches were applied: i) isolated haemocytes were treated for 30 min in physiological solution, ii) primary culture of haemocytes was treated for 22h. For in vivo experiment, mussels were exposed in static system for 72h. CdCl2 was used as positive control. The level of DNA damage was analyzed by Comet Assay IV software. CdCl2 induced significant increase in DNA damage in vitro and in vivo. Increase of damage was also detected with Eto in vivo and in primary cultures of haemocytes (in vitro). For 5–FU, the significant increase of DNA damage was detected only in vivo, while CP did not induced increase of DNA damage in vivo or in vitro. Lack of genotoxic effects of cytostatics in isolated haemocytes can be attributed to short period ...of exposure (30 min), while the lack of the effects of 5-FU and CP in primary cultures can be assigned to the mechanisms of action of these drugs.
Кључне речи:
cytostatics / genotoxicity / comet assay / freshwater musselsИзвор:
V Congress of the Serbian Genetic Society, 2014, 141-Издавач:
- Serbian Genetic Society, Belgrade, Serbia
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kolarević, Stoimir AU - Gacic, Zoran AU - Kostić, Jovana AU - Sunjog, Karolina AU - Kracun-Kolarevic, Margareta AU - Paunović, Momir AU - Knežević-Vukčević, Jelena AU - Vuković-Gačić, Branka PY - 2014 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2484 AB - The impact of 5-Fluorouracil (5-FU), Cisplatin (CP) and Etoposide (Eto) on DNA damage level was studied in vivo and in vitro on haemocytes of freshwater mussels Unio pictorum and U. tumidus using alkaline comet assay. For in vitro treatment, two different approaches were applied: i) isolated haemocytes were treated for 30 min in physiological solution, ii) primary culture of haemocytes was treated for 22h. For in vivo experiment, mussels were exposed in static system for 72h. CdCl2 was used as positive control. The level of DNA damage was analyzed by Comet Assay IV software. CdCl2 induced significant increase in DNA damage in vitro and in vivo. Increase of damage was also detected with Eto in vivo and in primary cultures of haemocytes (in vitro). For 5–FU, the significant increase of DNA damage was detected only in vivo, while CP did not induced increase of DNA damage in vivo or in vitro. Lack of genotoxic effects of cytostatics in isolated haemocytes can be attributed to short period of exposure (30 min), while the lack of the effects of 5-FU and CP in primary cultures can be assigned to the mechanisms of action of these drugs. PB - Serbian Genetic Society, Belgrade, Serbia C3 - V Congress of the Serbian Genetic Society T1 - Impact of in vivo and in vitro exposure to selected cytostatics on DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus SP - 141 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2484 ER -
@conference{ author = "Kolarević, Stoimir and Gacic, Zoran and Kostić, Jovana and Sunjog, Karolina and Kracun-Kolarevic, Margareta and Paunović, Momir and Knežević-Vukčević, Jelena and Vuković-Gačić, Branka", year = "2014", abstract = "The impact of 5-Fluorouracil (5-FU), Cisplatin (CP) and Etoposide (Eto) on DNA damage level was studied in vivo and in vitro on haemocytes of freshwater mussels Unio pictorum and U. tumidus using alkaline comet assay. For in vitro treatment, two different approaches were applied: i) isolated haemocytes were treated for 30 min in physiological solution, ii) primary culture of haemocytes was treated for 22h. For in vivo experiment, mussels were exposed in static system for 72h. CdCl2 was used as positive control. The level of DNA damage was analyzed by Comet Assay IV software. CdCl2 induced significant increase in DNA damage in vitro and in vivo. Increase of damage was also detected with Eto in vivo and in primary cultures of haemocytes (in vitro). For 5–FU, the significant increase of DNA damage was detected only in vivo, while CP did not induced increase of DNA damage in vivo or in vitro. Lack of genotoxic effects of cytostatics in isolated haemocytes can be attributed to short period of exposure (30 min), while the lack of the effects of 5-FU and CP in primary cultures can be assigned to the mechanisms of action of these drugs.", publisher = "Serbian Genetic Society, Belgrade, Serbia", journal = "V Congress of the Serbian Genetic Society", title = "Impact of in vivo and in vitro exposure to selected cytostatics on DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus", pages = "141", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2484" }
Kolarević, S., Gacic, Z., Kostić, J., Sunjog, K., Kracun-Kolarevic, M., Paunović, M., Knežević-Vukčević, J.,& Vuković-Gačić, B.. (2014). Impact of in vivo and in vitro exposure to selected cytostatics on DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in V Congress of the Serbian Genetic Society Serbian Genetic Society, Belgrade, Serbia., 141. https://hdl.handle.net/21.15107/rcub_rimsi_2484
Kolarević S, Gacic Z, Kostić J, Sunjog K, Kracun-Kolarevic M, Paunović M, Knežević-Vukčević J, Vuković-Gačić B. Impact of in vivo and in vitro exposure to selected cytostatics on DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus. in V Congress of the Serbian Genetic Society. 2014;:141. https://hdl.handle.net/21.15107/rcub_rimsi_2484 .
Kolarević, Stoimir, Gacic, Zoran, Kostić, Jovana, Sunjog, Karolina, Kracun-Kolarevic, Margareta, Paunović, Momir, Knežević-Vukčević, Jelena, Vuković-Gačić, Branka, "Impact of in vivo and in vitro exposure to selected cytostatics on DNA damage in haemocytes of freshwater mussels Unio pictorum and Unio tumidus" in V Congress of the Serbian Genetic Society (2014):141, https://hdl.handle.net/21.15107/rcub_rimsi_2484 .