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dc.creatorMilošević, Ivan
dc.creatorRadovanovic, Anita
dc.creatorDanilović Luković, Jelena
dc.creatorLuzajic Bozinovski, Tijana
dc.creatorSourice-Petit, Sophie
dc.creatorBeck-Cormier, Sarah
dc.creatorGuicheux, Jerome
dc.creatorKovačević Filipović, Milica
dc.date.accessioned2023-11-29T09:47:15Z
dc.date.available2023-11-29T09:47:15Z
dc.date.issued2018
dc.identifier.issn0567-8315
dc.identifier.urihttp://rimsi.imsi.bg.ac.rs/handle/123456789/2471
dc.description.abstractMaternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.sr
dc.language.isoensr
dc.publisherFaculty of Veterinary Medicine, University of Belgrade, Serbiasr
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175061/RSsr
dc.rightsopenAccesssr
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceActa Veterinaria-Beogradsr
dc.subjecttype 1, 2 and 10 collagenssr
dc.subjectTRAPsr
dc.subjectDMP-1sr
dc.subjecthypertrophic chondrocytessr
dc.subjectoffspring hypothyroidismsr
dc.titleEffect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formationsr
dc.typearticlesr
dc.rights.licenseBYsr
dc.citation.epage320
dc.citation.issue3
dc.citation.spage301
dc.citation.volume68
dc.identifier.doi10.2478/acve-2018-0026
dc.identifier.fulltexthttp://rimsi.imsi.bg.ac.rs/bitstream/id/6457/bitstream_6457.pdf
dc.type.versionpublishedVersionsr


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