Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation
2018
Preuzimanje 🢃
Autori
Milošević, IvanRadovanovic, Anita
Danilović Luković, Jelena
Luzajic Bozinovski, Tijana
Sourice-Petit, Sophie
Beck-Cormier, Sarah
Guicheux, Jerome
Kovačević Filipović, Milica
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
Maternal hypothyroidism in its overt form affects skeletal development of the offspring,
but these data are not available for the subclinical form which is becoming very frequent
among pregnant women. We hypothesized that the subclinical form of hypothyroidism
in rat dams, influences the process of offspring endochondral ossification affecting
proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups.
Seven-day-old male pups (n=18) derived from control dams and dams treated with a
low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water
during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia
proximal growth plate, expression of mRNA, immunohistochemical and histochemical
visualization of extracellular matrix components was performed. The length of the tibia
was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical
and overt form were lower while the amount of glyc...osaminoglycans was higher when
compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA
indicated altered osteoclasts function while lower expression of dentin matrix acid
protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised
bone formation in the overt form of hypothyroidism. The subclinical form of maternal
hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes
and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism
had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in
growth plate formation.
Ključne reči:
type 1, 2 and 10 collagens / TRAP / DMP-1 / hypertrophic chondrocytes / offspring hypothyroidismIzvor:
Acta Veterinaria-Beograd, 2018, 68, 3, 301-320Izdavač:
- Faculty of Veterinary Medicine, University of Belgrade, Serbia
Finansiranje / projekti:
Institucija/grupa
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Milošević, Ivan AU - Radovanovic, Anita AU - Danilović Luković, Jelena AU - Luzajic Bozinovski, Tijana AU - Sourice-Petit, Sophie AU - Beck-Cormier, Sarah AU - Guicheux, Jerome AU - Kovačević Filipović, Milica PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2471 AB - Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation. PB - Faculty of Veterinary Medicine, University of Belgrade, Serbia T2 - Acta Veterinaria-Beograd T1 - Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation EP - 320 IS - 3 SP - 301 VL - 68 DO - 10.2478/acve-2018-0026 ER -
@article{ author = "Milošević, Ivan and Radovanovic, Anita and Danilović Luković, Jelena and Luzajic Bozinovski, Tijana and Sourice-Petit, Sophie and Beck-Cormier, Sarah and Guicheux, Jerome and Kovačević Filipović, Milica", year = "2018", abstract = "Maternal hypothyroidism in its overt form affects skeletal development of the offspring, but these data are not available for the subclinical form which is becoming very frequent among pregnant women. We hypothesized that the subclinical form of hypothyroidism in rat dams, influences the process of offspring endochondral ossification affecting proliferation and differentiation of chondrocytes, osteoclasts and osteoblasts in pups. Seven-day-old male pups (n=18) derived from control dams and dams treated with a low dose (1.5 mg/L) or high dose (150 mg/L) of propylthiouracil in drinking water during pregnancy and lactation were used. Histomorphometric analysis of pups’ tibia proximal growth plate, expression of mRNA, immunohistochemical and histochemical visualization of extracellular matrix components was performed. The length of the tibia was reduced in hypothyroid pups. Secretion of type 2 and 10 collagens in the subclinical and overt form were lower while the amount of glycosaminoglycans was higher when compared with controls. Down-regulated tartrate resistant acid phosphatase mRNA indicated altered osteoclasts function while lower expression of dentin matrix acid protein–1 mRNA and reduced synthesis of type 1 collagen accentuated a compromised bone formation in the overt form of hypothyroidism. The subclinical form of maternal hypothyroidism had a negative effect on the differentiation of hypertrophic chondrocytes and calcified cartilage removal in 7-day-old pups. In addition, overt hypothyroidism had a negative effect on the proliferation of chondrocytes and deposition of osteoid. Both forms of hypothyroidism resulted in a decrease of tibia length due to changes in growth plate formation.", publisher = "Faculty of Veterinary Medicine, University of Belgrade, Serbia", journal = "Acta Veterinaria-Beograd", title = "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation", pages = "320-301", number = "3", volume = "68", doi = "10.2478/acve-2018-0026" }
Milošević, I., Radovanovic, A., Danilović Luković, J., Luzajic Bozinovski, T., Sourice-Petit, S., Beck-Cormier, S., Guicheux, J.,& Kovačević Filipović, M.. (2018). Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd Faculty of Veterinary Medicine, University of Belgrade, Serbia., 68(3), 301-320. https://doi.org/10.2478/acve-2018-0026
Milošević I, Radovanovic A, Danilović Luković J, Luzajic Bozinovski T, Sourice-Petit S, Beck-Cormier S, Guicheux J, Kovačević Filipović M. Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation. in Acta Veterinaria-Beograd. 2018;68(3):301-320. doi:10.2478/acve-2018-0026 .
Milošević, Ivan, Radovanovic, Anita, Danilović Luković, Jelena, Luzajic Bozinovski, Tijana, Sourice-Petit, Sophie, Beck-Cormier, Sarah, Guicheux, Jerome, Kovačević Filipović, Milica, "Effect of subclinical and overt form of rat maternal hypothyroidism on offspring endochondral bone formation" in Acta Veterinaria-Beograd, 68, no. 3 (2018):301-320, https://doi.org/10.2478/acve-2018-0026 . .