Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men
Authors
Janković, TamaraDanilović Luković, Jelena
Miler, Irena
Mitic, Ninoslav
Hajduković, Ljiljana
Janković, Miroslava
Article (Published version)
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Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct
tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of
different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal
proteins were determined aiming at the solubilization signature as an intrinsic multimolecular
marker and a new parameter suitable as a reference for the comparison of EVs populations in health and
disease.
Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that
maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase
(GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission
electron microscopy, electrophoresis, and lectin blot.
Results: Comparative data on prostasomes under normal physiology and conditions of low sp...erm count
revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly
part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral
membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns
mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/
GGT/concanavalin A-reactive glycoproteins.
Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the
surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular
environment, both of which contribute to the composition of spatial membrane arrangements.
Keywords:
Extracellular vesicles / detergent sensitivity / CD63 / CD9 / gamma-glutamyl transferase / molecular patterns / normozoospermia / oligozoospermiaSource:
Upsala journal of medical sciences, 2021, 126Publisher:
- Upsala Medical Society
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Janković, Tamara AU - Danilović Luković, Jelena AU - Miler, Irena AU - Mitic, Ninoslav AU - Hajduković, Ljiljana AU - Janković, Miroslava PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2464 AB - Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease. Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot. Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/ GGT/concanavalin A-reactive glycoproteins. Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements. PB - Upsala Medical Society T2 - Upsala journal of medical sciences T1 - Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men VL - 126 DO - 10.48101/ujms.v126.7673 ER -
@article{ author = "Janković, Tamara and Danilović Luković, Jelena and Miler, Irena and Mitic, Ninoslav and Hajduković, Ljiljana and Janković, Miroslava", year = "2021", abstract = "Background: Prostasomes, extracellular vesicles (EVs) abundantly present in seminal plasma, express distinct tetraspanins (TS) and galectin-3 (gal-3), which are supposed to shape their surface by an assembly of different molecular complexes. In this study, detergent-sensitivity patterns of membrane-associated prostasomal proteins were determined aiming at the solubilization signature as an intrinsic multimolecular marker and a new parameter suitable as a reference for the comparison of EVs populations in health and disease. Methods: Prostasomes were disrupted by Triton X-100 and analyzed by gel filtration under conditions that maintained complete solubilization. Redistribution of TS (CD63, CD9, and CD81), gal-3, gamma-glutamyltransferase (GGT), and distinct N-glycans was monitored using solid-phase lectin-binding assays, transmission electron microscopy, electrophoresis, and lectin blot. Results: Comparative data on prostasomes under normal physiology and conditions of low sperm count revealed similarity regarding the redistribution of distinct N-glycans and GGT, all presumed to be mainly part of the vesicle coat. In contrast to this, a greater difference was found in the redistribution of integral membrane proteins, exemplified by TS and gal-3. Accordingly, they were grouped into two molecular patterns mainly consisting of overlapped CD9/gal-3/wheat germ agglutinin-reactive glycoproteins and CD63/ GGT/concanavalin A-reactive glycoproteins. Conclusions: Solubilization signature can be considered as an all-inclusive distinction factor regarding the surface properties of a particular vesicle since it reflects the status of the parent cell and the extracellular environment, both of which contribute to the composition of spatial membrane arrangements.", publisher = "Upsala Medical Society", journal = "Upsala journal of medical sciences", title = "Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men", volume = "126", doi = "10.48101/ujms.v126.7673" }
Janković, T., Danilović Luković, J., Miler, I., Mitic, N., Hajduković, L.,& Janković, M.. (2021). Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men. in Upsala journal of medical sciences Upsala Medical Society., 126. https://doi.org/10.48101/ujms.v126.7673
Janković T, Danilović Luković J, Miler I, Mitic N, Hajduković L, Janković M. Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men. in Upsala journal of medical sciences. 2021;126. doi:10.48101/ujms.v126.7673 .
Janković, Tamara, Danilović Luković, Jelena, Miler, Irena, Mitic, Ninoslav, Hajduković, Ljiljana, Janković, Miroslava, "Assembly of tetraspanins, galectin-3, and distinct N-glycans defines the solubilization signature of seminal prostasomes from normozoospermic and oligozoospermic men" in Upsala journal of medical sciences, 126 (2021), https://doi.org/10.48101/ujms.v126.7673 . .