Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology
Аутори
Vitkovac, AleksandraPajić, Tanja
Bukumira, Marta
Stanić, Marina
Rabasovic, Mihailo
Todorović, Nataša
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with th...e Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.
Кључне речи:
Phycomyces / Imaging / TPEF / Machine-learning / IlastikИзвор:
2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023, 2023, 334-337Издавач:
- Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200007 (Универзитет у Београду, Институт за биолошка истраживања 'Синиша Станковић') (RS-MESTD-inst-2020-200007)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200178 (Универзитет у Београду, Биолошки факултет) (RS-MESTD-inst-2020-200178)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Science Fund of the Republic of Serbia, Grant 4545 (BioPhysFUN)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Vitkovac, Aleksandra AU - Pajić, Tanja AU - Bukumira, Marta AU - Stanić, Marina AU - Rabasovic, Mihailo AU - Todorović, Nataša PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2422 AB - Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response. PB - Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023 C3 - 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 T1 - Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology EP - 337 SP - 334 DO - 10.46793/ICCBI23.334V ER -
@conference{ author = "Vitkovac, Aleksandra and Pajić, Tanja and Bukumira, Marta and Stanić, Marina and Rabasovic, Mihailo and Todorović, Nataša", year = "2023", abstract = "Adaptive changes in mitochondrial morphology are associated with changes in the mitochondrial function and metabolic fitness of eukaryotic cells. We previously described in young hyphae of the filamentous fungus Phycomyces blakesleeanus a dramatic effect of an increase in ambient temperature during growth: a 3°C warmer environment compared with a control temperature of 22°C resulted in the appearance of long elongated (“tubular”) mitochondria accompanied by an increase in lipid droplet density. Here, we examined how cooler ambient temperature (18°C) during growth affects mitochondrial morphology in P. blakesleeanus compared with the control grown at 22°C. We used two-photon fluorescence imaging (TPEF) of live hyphae stained with the vital mitochondrial dye rhodamine 123. Extraction of relevant parameters (number, size, and shape of mitochondria) from TPEF images was performed using the Ilastik machine learning-based software. The suitability of the Ilastik analysis was compared with the Particle Analysis (ImageJ). Cold treatment resulted in the appearance of tubular mitochondrial morphology that was absent in the control group. Tubular mitochondrial morphology appears to be an adaptive feature that occurs in both warmer and colder conditions and is likely part of the stress response.", publisher = "Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023", journal = "2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023", title = "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology", pages = "337-334", doi = "10.46793/ICCBI23.334V" }
Vitkovac, A., Pajić, T., Bukumira, M., Stanić, M., Rabasovic, M.,& Todorović, N.. (2023). Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 Institute for Information Technologies, University of Kragujevac, Serbia, Jovana Cvijića bb, 2023., 334-337. https://doi.org/10.46793/ICCBI23.334V
Vitkovac A, Pajić T, Bukumira M, Stanić M, Rabasovic M, Todorović N. Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology. in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023. 2023;:334-337. doi:10.46793/ICCBI23.334V .
Vitkovac, Aleksandra, Pajić, Tanja, Bukumira, Marta, Stanić, Marina, Rabasovic, Mihailo, Todorović, Nataša, "Slight cooling during growth induced changes in filamentous fungi hypha mitochondrial morphology" in 2nd International Conference on Chemo and Bioinformatics, September 28-29, 2023. Kragujevac, Serbia, ICCBIKG 2023 (2023):334-337, https://doi.org/10.46793/ICCBI23.334V . .