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Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules
dc.creator | Kalauzi, Aleksandar | |
dc.creator | Mutavdžić, Dragosav | |
dc.creator | Đikanović, Daniela | |
dc.creator | Radotić, Ksenija | |
dc.creator | Jeremic, Milorad | |
dc.date.accessioned | 2022-04-05T14:13:57Z | |
dc.date.available | 2022-04-05T14:13:57Z | |
dc.date.issued | 2007 | |
dc.identifier.issn | 1053-0509 | |
dc.identifier.uri | http://rimsi.imsi.bg.ac.rs/handle/123456789/234 | |
dc.description.abstract | Having a valid mathematical model for structureless emission band shapes is important when deconvoluting fluorescence spectra of complex molecules. We propose a new asymmetric model for emission spectra of five organic molecules containing aromatic ring: catechol, coniferyl alcohol, hydroquinone, phenylalanine and tryptophan. For each molecule, a series of emission spectra, varying in excitation wavelength, were fitted with the new model as well as with two other analytical expressions: log-normal, described previously in the literature, and sigmoid-exponential. Their deconvolution into two, three and four Gaussian components was also performed, in order to estimate the number of symmetric components needed to obtain a better fitting quality than that of the asymmetric models. Four subtypes of the new model, as well as the log-normal one, did not differ significantly in their fitting errors, while the sigmoid-exponential model showed a significantly worse fit. Spectra of two mixtures: hydroquinone-coniferyl alcohol and hydroquinone-tryptophan were deconvoluted into two asymmetric and four Gaussian components. Positions of asymmetric components of mixtures matched those of separate molecules, while Gaussian did not. Component analysis of a polymer molecule, lignin, was also performed. In this more complex case asymmetric and Gaussian components also grouped in alternating positions. | en |
dc.publisher | Springer/Plenum Publishers, New York | |
dc.rights | restrictedAccess | |
dc.source | Journal of Fluorescence | |
dc.subject | nonlinear fitting | en |
dc.subject | monofluorophore | en |
dc.subject | mathematical models | en |
dc.subject | lignin | en |
dc.subject | binary mixture | en |
dc.title | Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules | en |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.epage | 329 | |
dc.citation.issue | 3 | |
dc.citation.other | 17(3): 319-329 | |
dc.citation.rank | M22 | |
dc.citation.spage | 319 | |
dc.citation.volume | 17 | |
dc.identifier.doi | 10.1007/s10895-007-0175-3 | |
dc.identifier.pmid | 17394054 | |
dc.identifier.scopus | 2-s2.0-34247573438 | |
dc.identifier.wos | 000246183900010 | |
dc.type.version | publishedVersion |