Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules
Само за регистроване кориснике
2007
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Having a valid mathematical model for structureless emission band shapes is important when deconvoluting fluorescence spectra of complex molecules. We propose a new asymmetric model for emission spectra of five organic molecules containing aromatic ring: catechol, coniferyl alcohol, hydroquinone, phenylalanine and tryptophan. For each molecule, a series of emission spectra, varying in excitation wavelength, were fitted with the new model as well as with two other analytical expressions: log-normal, described previously in the literature, and sigmoid-exponential. Their deconvolution into two, three and four Gaussian components was also performed, in order to estimate the number of symmetric components needed to obtain a better fitting quality than that of the asymmetric models. Four subtypes of the new model, as well as the log-normal one, did not differ significantly in their fitting errors, while the sigmoid-exponential model showed a significantly worse fit. Spectra of two mixtures: ...hydroquinone-coniferyl alcohol and hydroquinone-tryptophan were deconvoluted into two asymmetric and four Gaussian components. Positions of asymmetric components of mixtures matched those of separate molecules, while Gaussian did not. Component analysis of a polymer molecule, lignin, was also performed. In this more complex case asymmetric and Gaussian components also grouped in alternating positions.
Кључне речи:
nonlinear fitting / monofluorophore / mathematical models / lignin / binary mixtureИзвор:
Journal of Fluorescence, 2007, 17, 3, 319-329Издавач:
- Springer/Plenum Publishers, New York
DOI: 10.1007/s10895-007-0175-3
ISSN: 1053-0509
PubMed: 17394054
WoS: 000246183900010
Scopus: 2-s2.0-34247573438
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Kalauzi, Aleksandar AU - Mutavdžić, Dragosav AU - Đikanović, Daniela AU - Radotić, Ksenija AU - Jeremic, Milorad PY - 2007 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/234 AB - Having a valid mathematical model for structureless emission band shapes is important when deconvoluting fluorescence spectra of complex molecules. We propose a new asymmetric model for emission spectra of five organic molecules containing aromatic ring: catechol, coniferyl alcohol, hydroquinone, phenylalanine and tryptophan. For each molecule, a series of emission spectra, varying in excitation wavelength, were fitted with the new model as well as with two other analytical expressions: log-normal, described previously in the literature, and sigmoid-exponential. Their deconvolution into two, three and four Gaussian components was also performed, in order to estimate the number of symmetric components needed to obtain a better fitting quality than that of the asymmetric models. Four subtypes of the new model, as well as the log-normal one, did not differ significantly in their fitting errors, while the sigmoid-exponential model showed a significantly worse fit. Spectra of two mixtures: hydroquinone-coniferyl alcohol and hydroquinone-tryptophan were deconvoluted into two asymmetric and four Gaussian components. Positions of asymmetric components of mixtures matched those of separate molecules, while Gaussian did not. Component analysis of a polymer molecule, lignin, was also performed. In this more complex case asymmetric and Gaussian components also grouped in alternating positions. PB - Springer/Plenum Publishers, New York T2 - Journal of Fluorescence T1 - Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules EP - 329 IS - 3 SP - 319 VL - 17 DO - 10.1007/s10895-007-0175-3 ER -
@article{ author = "Kalauzi, Aleksandar and Mutavdžić, Dragosav and Đikanović, Daniela and Radotić, Ksenija and Jeremic, Milorad", year = "2007", abstract = "Having a valid mathematical model for structureless emission band shapes is important when deconvoluting fluorescence spectra of complex molecules. We propose a new asymmetric model for emission spectra of five organic molecules containing aromatic ring: catechol, coniferyl alcohol, hydroquinone, phenylalanine and tryptophan. For each molecule, a series of emission spectra, varying in excitation wavelength, were fitted with the new model as well as with two other analytical expressions: log-normal, described previously in the literature, and sigmoid-exponential. Their deconvolution into two, three and four Gaussian components was also performed, in order to estimate the number of symmetric components needed to obtain a better fitting quality than that of the asymmetric models. Four subtypes of the new model, as well as the log-normal one, did not differ significantly in their fitting errors, while the sigmoid-exponential model showed a significantly worse fit. Spectra of two mixtures: hydroquinone-coniferyl alcohol and hydroquinone-tryptophan were deconvoluted into two asymmetric and four Gaussian components. Positions of asymmetric components of mixtures matched those of separate molecules, while Gaussian did not. Component analysis of a polymer molecule, lignin, was also performed. In this more complex case asymmetric and Gaussian components also grouped in alternating positions.", publisher = "Springer/Plenum Publishers, New York", journal = "Journal of Fluorescence", title = "Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules", pages = "329-319", number = "3", volume = "17", doi = "10.1007/s10895-007-0175-3" }
Kalauzi, A., Mutavdžić, D., Đikanović, D., Radotić, K.,& Jeremic, M.. (2007). Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules. in Journal of Fluorescence Springer/Plenum Publishers, New York., 17(3), 319-329. https://doi.org/10.1007/s10895-007-0175-3
Kalauzi A, Mutavdžić D, Đikanović D, Radotić K, Jeremic M. Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules. in Journal of Fluorescence. 2007;17(3):319-329. doi:10.1007/s10895-007-0175-3 .
Kalauzi, Aleksandar, Mutavdžić, Dragosav, Đikanović, Daniela, Radotić, Ksenija, Jeremic, Milorad, "Application of asymmetric model in analysis of fluorescence spectra of biologically important molecules" in Journal of Fluorescence, 17, no. 3 (2007):319-329, https://doi.org/10.1007/s10895-007-0175-3 . .