Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)
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2007
Authors
Bogdanović Pristov, Jelena
Milosavić, Nenad
Prodanović, Radivoje

Ducic, Tanja

Radotić, Ksenija

Article (Published version)

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Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones.
Keywords:
superoxide dismutase / peroxidase / catechol oxidase / catalaseSource:
Biochemical Systematics and Ecology, 2007, 35, 5, 263-273Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/j.bse.2006.12.001
ISSN: 0305-1978
WoS: 000246291300002
Scopus: 2-s2.0-33947284863
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Bogdanović Pristov, Jelena AU - Milosavić, Nenad AU - Prodanović, Radivoje AU - Ducic, Tanja AU - Radotić, Ksenija PY - 2007 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/233 AB - Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones. PB - Pergamon-Elsevier Science Ltd, Oxford T2 - Biochemical Systematics and Ecology T1 - Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye) EP - 273 IS - 5 SP - 263 VL - 35 DO - 10.1016/j.bse.2006.12.001 ER -
@article{ author = "Bogdanović Pristov, Jelena and Milosavić, Nenad and Prodanović, Radivoje and Ducic, Tanja and Radotić, Ksenija", year = "2007", abstract = "Variations were studied of the activity and isoenzyme patterns of soluble peroxidase, catalase, catechol oxidase and superoxide dismutase, in needles of the Balkan endemic conifer Serbian spruce, Picea omorika (Panc.) Purkinye. The samples were collected from the natural habitat of the species, Mt. Tara. Seasonal changes were found to affect enzymatic activities and isoenzyme profiles. Total protein content was significantly lower in the summer than in other seasons. Several isoforms of peroxidase, catechol oxidase and superoxide dismutase (SOD), as well as two catalase isoenzymes were detected. The number of peroxidase isoenzymes was greatest during the vegetative season. Catalase and catechol oxidase peaked in summer and spring, respectively. Total SOD and Mn-SOD activities were significantly higher in the winter samples than the summer ones.", publisher = "Pergamon-Elsevier Science Ltd, Oxford", journal = "Biochemical Systematics and Ecology", title = "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)", pages = "273-263", number = "5", volume = "35", doi = "10.1016/j.bse.2006.12.001" }
Bogdanović Pristov, J., Milosavić, N., Prodanović, R., Ducic, T.,& Radotić, K.. (2007). Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). in Biochemical Systematics and Ecology Pergamon-Elsevier Science Ltd, Oxford., 35(5), 263-273. https://doi.org/10.1016/j.bse.2006.12.001
Bogdanović Pristov J, Milosavić N, Prodanović R, Ducic T, Radotić K. Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye). in Biochemical Systematics and Ecology. 2007;35(5):263-273. doi:10.1016/j.bse.2006.12.001 .
Bogdanović Pristov, Jelena, Milosavić, Nenad, Prodanović, Radivoje, Ducic, Tanja, Radotić, Ksenija, "Variability of antioxidant enzyme activity and isoenzyme profile in needles of Serbian spruce (Picea omorika (Panc.) Purkinye)" in Biochemical Systematics and Ecology, 35, no. 5 (2007):263-273, https://doi.org/10.1016/j.bse.2006.12.001 . .