Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus
Authors
Žižić, Milan
Stanić, Marina

AQUILANTI, Giuliana
Bajuk-Bogdanovic, Danica
Branković, Goran

Rodić, Ivanka
Živić, Miroslav
Zakrzewska, Joanna
Article (Updated Version)

Co-owner Consortium consisting of GDCh, SFC, SEQA, RSEQ, DAS/SCS, ASAC, SCI, PTC and Springer-Verlag GmbH Berlin Heidelberg
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Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation.
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 ...mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time.
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy
Keywords:
Biogenic selenium nanoparticles · / Phycomyces blakesleeanus · / Volatile selenium compounds / Selenium biotransformationSource:
Analytical and Bianalytical Chemistry, 2022, 414, 20, 6213-6222.Publisher:
- Springer-Verlag GmbH Berlin Heidelberg
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200051 (Institute of General and Physical Chemistry, Belgrade) (RS-200051)
- "info:eu-repo/grantAgreement/MESTD/inst-2020/200178/RS// (RS-200178)
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Žižić, Milan AU - Stanić, Marina AU - AQUILANTI, Giuliana AU - Bajuk-Bogdanovic, Danica AU - Branković, Goran AU - Rodić, Ivanka AU - Živić, Miroslav AU - Zakrzewska, Joanna PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2106 AB - Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy PB - Springer-Verlag GmbH Berlin Heidelberg T2 - Analytical and Bianalytical Chemistry T1 - Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus EP - 6222. IS - 20 SP - 6213 VL - 414 DO - 10.1007/s00216-022-04191-4 ER -
@article{ author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna", year = "2022", abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy", publisher = "Springer-Verlag GmbH Berlin Heidelberg", journal = "Analytical and Bianalytical Chemistry", title = "Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus", pages = "6222.-6213", number = "20", volume = "414", doi = "10.1007/s00216-022-04191-4" }
Žižić, M., Stanić, M., AQUILANTI, G., Bajuk-Bogdanovic, D., Branković, G., Rodić, I., Živić, M.,& Zakrzewska, J.. (2022). Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry Springer-Verlag GmbH Berlin Heidelberg., 414(20), 6213-6222.. https://doi.org/10.1007/s00216-022-04191-4
Žižić M, Stanić M, AQUILANTI G, Bajuk-Bogdanovic D, Branković G, Rodić I, Živić M, Zakrzewska J. Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry. 2022;414(20):6213-6222.. doi:10.1007/s00216-022-04191-4 .
Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Bajuk-Bogdanovic, Danica, Branković, Goran, Rodić, Ivanka, Živić, Miroslav, Zakrzewska, Joanna, "Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus" in Analytical and Bianalytical Chemistry, 414, no. 20 (2022):6213-6222., https://doi.org/10.1007/s00216-022-04191-4 . .