Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus
Аутори
Žižić, MilanStanić, Marina
AQUILANTI, Giuliana
Bajuk-Bogdanovic, Danica
Branković, Goran
Rodić, Ivanka
Živić, Miroslav
Zakrzewska, Joanna
Чланак у часопису (Коригована верзија)
,
Co-owner Consortium consisting of GDCh, SFC, SEQA, RSEQ, DAS/SCS, ASAC, SCI, PTC and Springer-Verlag GmbH Berlin Heidelberg
Метаподаци
Приказ свих података о документуАпстракт
Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms
and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation.
In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as
well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red
coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of
SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter
of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium
revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 ...mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile
dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time.
Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer
chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy
Кључне речи:
Biogenic selenium nanoparticles · / Phycomyces blakesleeanus · / Volatile selenium compounds / Selenium biotransformationИзвор:
Analytical and Bianalytical Chemistry, 2022, 414, 20, 6213-6222.Издавач:
- Springer-Verlag GmbH Berlin Heidelberg
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200051 (Институт за општу и физичку хемију, Београд) (RS-MESTD-inst-2020-200051)
- "info:eu-repo/grantAgreement/MESTD/inst-2020/200178/RS// (RS-MESTD-inst-2020-200178)
Институција/група
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Žižić, Milan AU - Stanić, Marina AU - AQUILANTI, Giuliana AU - Bajuk-Bogdanovic, Danica AU - Branković, Goran AU - Rodić, Ivanka AU - Živić, Miroslav AU - Zakrzewska, Joanna PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2106 AB - Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy PB - Springer-Verlag GmbH Berlin Heidelberg T2 - Analytical and Bianalytical Chemistry T1 - Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus EP - 6222. IS - 20 SP - 6213 VL - 414 DO - 10.1007/s00216-022-04191-4 ER -
@article{ author = "Žižić, Milan and Stanić, Marina and AQUILANTI, Giuliana and Bajuk-Bogdanovic, Danica and Branković, Goran and Rodić, Ivanka and Živić, Miroslav and Zakrzewska, Joanna", year = "2022", abstract = "Biotransformation of toxic selenium ions to non-toxic species has been mainly focused on biofortifcation of microorganisms and production of selenium nanoparticles (SeNPs), while far less attention is paid to the mechanisms of transformation. In this study, we applied a combination of analytical techniques with the aim of characterizing the SeNPs themselves as well as monitoring the course of selenium transformation in the mycelium of the fungus Phycomyces blakesleeanus. Red coloration and pungent odor that appeared after only a few hours of incubation with 10 mM Se+4 indicate the formation of SeNPs and volatile methylated selenium compounds. SEM–EDS confrmed pure selenium NPs with an average diameter of 57 nm, which indicates potentially very good medical, optical, and photoelectric characteristics. XANES of mycelium revealed concentration-dependent mechanisms of reduction, where 0.5 mM Se+4 led to the predominant formation of Se–S containing organic molecules, while 10 mM Se+4 induced production of biomethylated selenide (Se−2) in the form of volatile dimethylselenide (DMSe) and selenium nanoparticles (SeNPs), with the SeNPs/DMSe ratio rising with incubation time. Several structural forms of elemental selenium, predominantly monoclinic Se8 chains, together with trigonal Se polymer chain, Se8 and Se6 ring structures, were detected by Raman spectroscopy", publisher = "Springer-Verlag GmbH Berlin Heidelberg", journal = "Analytical and Bianalytical Chemistry", title = "Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus", pages = "6222.-6213", number = "20", volume = "414", doi = "10.1007/s00216-022-04191-4" }
Žižić, M., Stanić, M., AQUILANTI, G., Bajuk-Bogdanovic, D., Branković, G., Rodić, I., Živić, M.,& Zakrzewska, J.. (2022). Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry Springer-Verlag GmbH Berlin Heidelberg., 414(20), 6213-6222.. https://doi.org/10.1007/s00216-022-04191-4
Žižić M, Stanić M, AQUILANTI G, Bajuk-Bogdanovic D, Branković G, Rodić I, Živić M, Zakrzewska J. Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus. in Analytical and Bianalytical Chemistry. 2022;414(20):6213-6222.. doi:10.1007/s00216-022-04191-4 .
Žižić, Milan, Stanić, Marina, AQUILANTI, Giuliana, Bajuk-Bogdanovic, Danica, Branković, Goran, Rodić, Ivanka, Živić, Miroslav, Zakrzewska, Joanna, "Biotransformation of selenium in the mycelium of the fungus Phycomyces blakesleeanus" in Analytical and Bianalytical Chemistry, 414, no. 20 (2022):6213-6222., https://doi.org/10.1007/s00216-022-04191-4 . .