Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’
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2022
Authors
Jelušić, Aleksandra
Popović Milovanović, Tatjana
Mitrović, Petar
Stanisavljević, Rade
Janakiev, Tamara
Fira, Đorđe
Dimkić, Ivica
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The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid
formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i)
seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were
used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar trea...tment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found
between disease intensity and cabbage heads’ weight.
Keywords:
biological control / Bacillus velezensis / Pseudomonas orientalis / black rot / Xanthomonas campestris pv. campestrisSource:
14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy, 2022, 147-147Publisher:
- Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-200010)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-200032)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200178 (University of Belgrade, Faculty of Biology) (RS-200178)
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Jelušić, Aleksandra AU - Popović Milovanović, Tatjana AU - Mitrović, Petar AU - Stanisavljević, Rade AU - Janakiev, Tamara AU - Fira, Đorđe AU - Dimkić, Ivica PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2047 AB - The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i) seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar treatment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found between disease intensity and cabbage heads’ weight. PB - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini C3 - 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy T1 - Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’ EP - 147 SP - 147 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2047 ER -
@conference{ author = "Jelušić, Aleksandra and Popović Milovanović, Tatjana and Mitrović, Petar and Stanisavljević, Rade and Janakiev, Tamara and Fira, Đorđe and Dimkić, Ivica", year = "2022", abstract = "The potential of two biocontrol strains, Bacillus velezensis X5-2 and Pseudomonas orientalis X2-1P to control black rot caused by Xanthomonas campestris pv. campestris was evaluated in vivo on autochthonous cabbage cultivar ‘Futoški’, under the condition of natural infection. Liquid formulations of biocontrol strains were prepared in fermenters (B. velezensis - 1010 CFU mL-1, P. orientalis - 109 CFU mL-1) and diluted with water in a 1:5 ratio, before use. Treatments were performed with each strain and their mixture (1:1). The experiment included the following: (i) seed treatments (15, 30, 60 min), (ii) foliar treatments (three treatments: when the first symptoms appeared and remaining every two weeks), and (iii) combined seed and foliar treatments. Experiments were conducted in four replicates, with 10 plants per replicate. Two controls were used, untreated and conventionally treated cabbage (pesticides). Assessment of disease intensity was rated one month after the last foliar treatment, using the Horsfall-Barratt scale (1-12). During ripening, cabbage heads’ weights were measured and data were statistically processed (Minitab). All performed treatments were effective in the control of black rot (85.7-98.9%). The highest efficacy was obtained in combined seed (60 min) and foliar treatments with P. orientalis, as well as its mixture with B. velezensis (98.9% both). All treatments influenced the increase of cabbage heads’ weight (up to 1.3 times), with the highest found in combined treatments (60 min seed + foliar) using a mixture of B. velezensis and P. orientalis. A negative correlation (P≤0.05) was found between disease intensity and cabbage heads’ weight.", publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini", journal = "14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy", title = "Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’", pages = "147-147", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2047" }
Jelušić, A., Popović Milovanović, T., Mitrović, P., Stanisavljević, R., Janakiev, T., Fira, Đ.,& Dimkić, I.. (2022). Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’. in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria; Progetto grafico - editoriale esecutivo, Diletta Pini., 147-147. https://hdl.handle.net/21.15107/rcub_rimsi_2047
Jelušić A, Popović Milovanović T, Mitrović P, Stanisavljević R, Janakiev T, Fira Đ, Dimkić I. Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’. in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy. 2022;:147-147. https://hdl.handle.net/21.15107/rcub_rimsi_2047 .
Jelušić, Aleksandra, Popović Milovanović, Tatjana, Mitrović, Petar, Stanisavljević, Rade, Janakiev, Tamara, Fira, Đorđe, Dimkić, Ivica, "Biocontrol of black rot on autochthonous cabbage cultivar ‘Futoški’" in 14th International Conference on Plant Pathogenic Bacteria, Assisi, Italy (2022):147-147, https://hdl.handle.net/21.15107/rcub_rimsi_2047 .