αVβ3-Integrin and mitochondria mediate astrocyte response to autoreactive immune cells
Authorized Users Only
2023
Authors
Milicevic, KatarinaBataveljic, Danijela
Bogdanović Pristov, Jelena

Andjus, Pavle
Nikolic, Ljiljana
Conference object (Published version)

Serbian Neuroscience Society and associates
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Show full item recordAbstract
The astrocytic network maintains homeostasis in the central nervous system (CNS)
through interactions with neighboring cells. In the CNS autoimmune disease, multiple
sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions.
Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs)
of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS,
rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor
(P2X7R). In the present study we further defined the mechanisms responsible for
astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in
vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured
naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal
cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in
the initial contact of astrocytes with CNS-IICs, since... blocking αvβ3-integrin reduced
the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial
Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the
intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs,
demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction.
Overall, our study expands the understanding of astrocytes’ interaction with
autoreactive immune cells that are present in their local environment in an
autoimmune disease. This offers a new conceptual framework for considering direct
astrocyte–immune cell interaction to design new strategies for therapy development in
the treatment of MS.
Keywords:
astrocyte / multiple sclerosis / P2X7 receptorSource:
8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS, 2023, 101-Publisher:
- Serbian Neuroscience Society
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-200007)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200178 (University of Belgrade, Faculty of Biology) (RS-200178)
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Milicevic, Katarina AU - Bataveljic, Danijela AU - Bogdanović Pristov, Jelena AU - Andjus, Pavle AU - Nikolic, Ljiljana PY - 2023 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2014 AB - The astrocytic network maintains homeostasis in the central nervous system (CNS) through interactions with neighboring cells. In the CNS autoimmune disease, multiple sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor (P2X7R). In the present study we further defined the mechanisms responsible for astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. Overall, our study expands the understanding of astrocytes’ interaction with autoreactive immune cells that are present in their local environment in an autoimmune disease. This offers a new conceptual framework for considering direct astrocyte–immune cell interaction to design new strategies for therapy development in the treatment of MS. PB - Serbian Neuroscience Society C3 - 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS T1 - αVβ3-Integrin and mitochondria mediate astrocyte response to autoreactive immune cells SP - 101 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2014 ER -
@conference{ author = "Milicevic, Katarina and Bataveljic, Danijela and Bogdanović Pristov, Jelena and Andjus, Pavle and Nikolic, Ljiljana", year = "2023", abstract = "The astrocytic network maintains homeostasis in the central nervous system (CNS) through interactions with neighboring cells. In the CNS autoimmune disease, multiple sclerosis (MS), neuroinflammatory conditions modulate these cell-to-cell interactions. Our previous work revealed that the immune cells infiltrated into the CNS (CNS-IICs) of experimental autoimmune encemphalomyelitis (EAE) rat, an animal model of MS, rapidly alter the activity pattern of astrocytes by activating the glial P2X7 receptor (P2X7R). In the present study we further defined the mechanisms responsible for astrocytes’ activation in the presence of CNS-IICs. For this purpose, we used an in vitro experimental setup and monitored Ca2+ dynamics in Fluo-4-labeled cultured naïve astrocytes following brief bath application of CNS-IICs isolated from the spinal cord of the EAE rat. Our data indicate that the astroglial αvβ3-integrin is involved in the initial contact of astrocytes with CNS-IICs, since blocking αvβ3-integrin reduced the expected astrocytic Ca2+ response. Furthermore, blocking of mitochondrial Na+/Ca2+- and H+/Ca2+- exchangers in astrocytes promoted an augmentation of the intracellular Ca2+ increase and a higher ATP release after brief exposure to CNS-IICs, demonstrating that mitochondria regulate the astrocyte-CNS IICs cell-cell interaction. Overall, our study expands the understanding of astrocytes’ interaction with autoreactive immune cells that are present in their local environment in an autoimmune disease. This offers a new conceptual framework for considering direct astrocyte–immune cell interaction to design new strategies for therapy development in the treatment of MS.", publisher = "Serbian Neuroscience Society", journal = "8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS", title = "αVβ3-Integrin and mitochondria mediate astrocyte response to autoreactive immune cells", pages = "101", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2014" }
Milicevic, K., Bataveljic, D., Bogdanović Pristov, J., Andjus, P.,& Nikolic, L.. (2023). αVβ3-Integrin and mitochondria mediate astrocyte response to autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS Serbian Neuroscience Society., 101. https://hdl.handle.net/21.15107/rcub_rimsi_2014
Milicevic K, Bataveljic D, Bogdanović Pristov J, Andjus P, Nikolic L. αVβ3-Integrin and mitochondria mediate astrocyte response to autoreactive immune cells. in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS. 2023;:101. https://hdl.handle.net/21.15107/rcub_rimsi_2014 .
Milicevic, Katarina, Bataveljic, Danijela, Bogdanović Pristov, Jelena, Andjus, Pavle, Nikolic, Ljiljana, "αVβ3-Integrin and mitochondria mediate astrocyte response to autoreactive immune cells" in 8th CONGRESS OF SERBIAN NEUROSCIENCE SOCIETY with international participation 31 May – 2 June 2023. Belgrade, Serbia - BOOK OF ABSTRACTS (2023):101, https://hdl.handle.net/21.15107/rcub_rimsi_2014 .