PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE WITH A MOVABLE STAGE
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2022
Authors
Nikolic, LjiljanaVidovic, Marija
Todorovic, Natasa
Petkovic, Branka
Stojadinovic, Gordana
Martac, Ljiljana
Bogdanović Pristov, Jelena

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Investigating membrane properties of plants is a challenging task, considering that success of
experiments is highly dependent on the possibility to isolate metabolically active protoplasts that
can withstand membrane current recordings. The aim of the present work is to obtain viable
protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch
clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts
with preserved membrane integrity suitable for electrophysiological experiments. We applied a
custom approach for patch-clamping protoplasts using a microscope with a movable microscope
stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.
Keywords:
protoplast / patch-clamp / upright microscope / membrane currentSource:
PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings), 2022, 1, 271-274Publisher:
- Society of Physical Chemists of Serbia
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-200007)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-200042)
Note:
- kategorija M33
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Nikolic, Ljiljana AU - Vidovic, Marija AU - Todorovic, Natasa AU - Petkovic, Branka AU - Stojadinovic, Gordana AU - Martac, Ljiljana AU - Bogdanović Pristov, Jelena PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/2008 AB - Investigating membrane properties of plants is a challenging task, considering that success of experiments is highly dependent on the possibility to isolate metabolically active protoplasts that can withstand membrane current recordings. The aim of the present work is to obtain viable protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts with preserved membrane integrity suitable for electrophysiological experiments. We applied a custom approach for patch-clamping protoplasts using a microscope with a movable microscope stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts. PB - Society of Physical Chemists of Serbia C3 - PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings) T1 - PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE WITH A MOVABLE STAGE EP - 274 SP - 271 VL - 1 UR - https://hdl.handle.net/21.15107/rcub_rimsi_2008 ER -
@conference{ author = "Nikolic, Ljiljana and Vidovic, Marija and Todorovic, Natasa and Petkovic, Branka and Stojadinovic, Gordana and Martac, Ljiljana and Bogdanović Pristov, Jelena", year = "2022", abstract = "Investigating membrane properties of plants is a challenging task, considering that success of experiments is highly dependent on the possibility to isolate metabolically active protoplasts that can withstand membrane current recordings. The aim of the present work is to obtain viable protoplasts derived from root cells of Pisum sativum that can be used for the whole-cell patch clamp. We designed the procedure of the pea protoplasts isolation that delivers stable protoplasts with preserved membrane integrity suitable for electrophysiological experiments. We applied a custom approach for patch-clamping protoplasts using a microscope with a movable microscope stage. We recorded prominent inward and prominent outward types of membrane current profiles of protoplasts. Obtained data indicate that optimized isolation protocol and custom system for patch clamping, can be applied to study membrane properties of root protoplasts.", publisher = "Society of Physical Chemists of Serbia", journal = "PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings)", title = "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE WITH A MOVABLE STAGE", pages = "274-271", volume = "1", url = "https://hdl.handle.net/21.15107/rcub_rimsi_2008" }
Nikolic, L., Vidovic, M., Todorovic, N., Petkovic, B., Stojadinovic, G., Martac, L.,& Bogdanović Pristov, J.. (2022). PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings) Society of Physical Chemists of Serbia., 1, 271-274. https://hdl.handle.net/21.15107/rcub_rimsi_2008
Nikolic L, Vidovic M, Todorovic N, Petkovic B, Stojadinovic G, Martac L, Bogdanović Pristov J. PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE WITH A MOVABLE STAGE. in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings). 2022;1:271-274. https://hdl.handle.net/21.15107/rcub_rimsi_2008 .
Nikolic, Ljiljana, Vidovic, Marija, Todorovic, Natasa, Petkovic, Branka, Stojadinovic, Gordana, Martac, Ljiljana, Bogdanović Pristov, Jelena, "PROTOPLAST PATCH-CLAMPING USING AN UPRIGHT MICROSCOPE WITH A MOVABLE STAGE" in PHYSICAL CHEMISTRY 2022, 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry (Proceedings), 1 (2022):271-274, https://hdl.handle.net/21.15107/rcub_rimsi_2008 .