Removal of phenolic compounds from wastewaters has been investigated by many researchers and it has been shown that horse radish peroxidase can react with aqueous phenolic compound in the presence of hydrogen peroxide and form non-soluble materials that could be easily removed from the aqueous phase. Main disadvantage of this process is enzyme inactivation. In order to increase stability of enzyme and yield of biocatalytic reaction peroxidases were immobilized on different materials. The aim of this study was to test different macroporous glycidyl methacrylates as carriers for enzyme immobilization. Immobilization was performed using carriers with different pore sizes (40, 50, 120 and 200 nm) and glutaraldehyde as activation method. All immobilized enzymes were characterized using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and specific activity and immobilization yield were determined. Our data demonstrate that the best carrier for immobilization of horse radish... peroxidase was SGE EDA 20/12 with pore size of 120 nm.
TY - CONF
AU - Prodanović, Olivera
AU - Prokopijević, Miloš
AU - Spasojević, Dragica
AU - Prodanović, Radivoje
AU - Stojanović, Željko
AU - Radotić, Ksenija
PY - 2011
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1995
AB - Removal of phenolic compounds from wastewaters has been investigated by many researchers and it has been shown that horse radish peroxidase can react with aqueous phenolic compound in the presence of hydrogen peroxide and form non-soluble materials that could be easily removed from the aqueous phase. Main disadvantage of this process is enzyme inactivation. In order to increase stability of enzyme and yield of biocatalytic reaction peroxidases were immobilized on different materials. The aim of this study was to test different macroporous glycidyl methacrylates as carriers for enzyme immobilization. Immobilization was performed using carriers with different pore sizes (40, 50, 120 and 200 nm) and glutaraldehyde as activation method. All immobilized enzymes were characterized using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and specific activity and immobilization yield were determined. Our data demonstrate that the best carrier for immobilization of horse radish peroxidase was SGE EDA 20/12 with pore size of 120 nm.
PB - AP Print, Podgorica
C3 - Naučni skup sa medjunarodnim učešćem: Zaštita prirode u XXI vijeku, Žabljak, Crna Gora
T1 - Immobilization of horse radish peroxidase on different macroporous glycydil methacrylates for wastewater treatment
EP - 711
SP - 709
VL - 2
UR - https://hdl.handle.net/21.15107/rcub_rimsi_1995
ER -
@conference{
author = "Prodanović, Olivera and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Stojanović, Željko and Radotić, Ksenija",
year = "2011",
abstract = "Removal of phenolic compounds from wastewaters has been investigated by many researchers and it has been shown that horse radish peroxidase can react with aqueous phenolic compound in the presence of hydrogen peroxide and form non-soluble materials that could be easily removed from the aqueous phase. Main disadvantage of this process is enzyme inactivation. In order to increase stability of enzyme and yield of biocatalytic reaction peroxidases were immobilized on different materials. The aim of this study was to test different macroporous glycidyl methacrylates as carriers for enzyme immobilization. Immobilization was performed using carriers with different pore sizes (40, 50, 120 and 200 nm) and glutaraldehyde as activation method. All immobilized enzymes were characterized using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and specific activity and immobilization yield were determined. Our data demonstrate that the best carrier for immobilization of horse radish peroxidase was SGE EDA 20/12 with pore size of 120 nm.",
publisher = "AP Print, Podgorica",
journal = "Naučni skup sa medjunarodnim učešćem: Zaštita prirode u XXI vijeku, Žabljak, Crna Gora",
title = "Immobilization of horse radish peroxidase on different macroporous glycydil methacrylates for wastewater treatment",
pages = "711-709",
volume = "2",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1995"
}
Prodanović, O., Prokopijević, M., Spasojević, D., Prodanović, R., Stojanović, Ž.,& Radotić, K.. (2011). Immobilization of horse radish peroxidase on different macroporous glycydil methacrylates for wastewater treatment. in Naučni skup sa medjunarodnim učešćem: Zaštita prirode u XXI vijeku, Žabljak, Crna Gora
AP Print, Podgorica., 2, 709-711.
https://hdl.handle.net/21.15107/rcub_rimsi_1995
Prodanović O, Prokopijević M, Spasojević D, Prodanović R, Stojanović Ž, Radotić K. Immobilization of horse radish peroxidase on different macroporous glycydil methacrylates for wastewater treatment. in Naučni skup sa medjunarodnim učešćem: Zaštita prirode u XXI vijeku, Žabljak, Crna Gora. 2011;2:709-711.
https://hdl.handle.net/21.15107/rcub_rimsi_1995 .
Prodanović, Olivera, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Stojanović, Željko, Radotić, Ksenija, "Immobilization of horse radish peroxidase on different macroporous glycydil methacrylates for wastewater treatment" in Naučni skup sa medjunarodnim učešćem: Zaštita prirode u XXI vijeku, Žabljak, Crna Gora, 2 (2011):709-711,
https://hdl.handle.net/21.15107/rcub_rimsi_1995 .
