In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes
Authors
Štrbac, Filip
Bosco, Antonio
Stojanović, Dragica
Ratajac, Radomir
Simin, Nataša

Orčić, Dejan
Petrović, Kosta
Cringoli, Giuseppe
Rinaldi, Laura
Conference object (Published version)

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The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distil...led water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.
Keywords:
anthelmintic resistance / essential oil / Citrus / GC-MS / gastrointestinal nematodesSource:
Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan, 2022, 27-27Publisher:
- Unique Scientific Publishers, Faisalabad, Pakistan
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Štrbac, Filip AU - Bosco, Antonio AU - Stojanović, Dragica AU - Ratajac, Radomir AU - Simin, Nataša AU - Orčić, Dejan AU - Petrović, Kosta AU - Cringoli, Giuseppe AU - Rinaldi, Laura PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1970 AB - The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings. PB - Unique Scientific Publishers, Faisalabad, Pakistan C3 - Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan T1 - In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes EP - 27 SP - 27 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1970 ER -
@conference{ author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura", year = "2022", abstract = "The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.", publisher = "Unique Scientific Publishers, Faisalabad, Pakistan", journal = "Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan", title = "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes", pages = "27-27", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1970" }
Štrbac, F., Bosco, A., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan Unique Scientific Publishers, Faisalabad, Pakistan., 27-27. https://hdl.handle.net/21.15107/rcub_rimsi_1970
Štrbac F, Bosco A, Stojanović D, Ratajac R, Simin N, Orčić D, Petrović K, Cringoli G, Rinaldi L. In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan. 2022;:27-27. https://hdl.handle.net/21.15107/rcub_rimsi_1970 .
Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes" in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan (2022):27-27, https://hdl.handle.net/21.15107/rcub_rimsi_1970 .