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In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes

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2022
bitstream_4964.pdf (1.683Mb)
Authors
Štrbac, Filip
Bosco, Antonio
Stojanović, Dragica
Ratajac, Radomir
Simin, Nataša
Orčić, Dejan
Petrović, Kosta
Cringoli, Giuseppe
Rinaldi, Laura
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Abstract
The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distil...led water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.

Keywords:
anthelmintic resistance / essential oil / Citrus / GC-MS / gastrointestinal nematodes
Source:
Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan, 2022, 27-27
Publisher:
  • Unique Scientific Publishers, Faisalabad, Pakistan

ISBN: 978-969-2201-03-2

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rimsi_1970
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1970
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - CONF
AU  - Štrbac, Filip
AU  - Bosco, Antonio
AU  - Stojanović, Dragica
AU  - Ratajac, Radomir
AU  - Simin, Nataša
AU  - Orčić, Dejan
AU  - Petrović, Kosta
AU  - Cringoli, Giuseppe
AU  - Rinaldi, Laura
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1970
AB  - The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.
PB  - Unique Scientific Publishers, Faisalabad, Pakistan
C3  - Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan
T1  - In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes
EP  - 27
SP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1970
ER  - 
@conference{
author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Ratajac, Radomir and Simin, Nataša and Orčić, Dejan and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura",
year = "2022",
abstract = "The management of infections caused by sheep gastrointestinal nematodes (GINs) is a challenging task due to the development of anthelmintic resistance on commercial drugs. The urgency of the situation justifies the search for alternatives, which includes plant essential oils (EOs). This study aimed to evaluate the in vitro effect of two EOs, Citrus medica L. and Citrus sinensis L. by using the egg hatch test (EHT). For both tested EOs, EHT was performed at eight
concentrations: 50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL. The positive control was thiabendazole at the two lowest concentrations used for tested samples, and the negative controls were 3% (v/v) Tween 80 and distilled water. Both tested samples showed ovicidal potential against sheep GINs with inhibition of egg hatchability varied from 12.3-95.0% and 14.7-86.3% for EOs of C. medica and C. sinensis, respectively. For the positive control, results varied from 95.0-96.3%, for the 3% Tween 9.0-14.7%, and distilled water 4.0-4.7%. The chemical composition of EOs was determined by GC-MS. The results showed that the main ingredients of C. medica EO were limonene (75 58%), β-pinene (11 57%), and γ-terpinene (8.66%), and of C. sinensis were limonene (96.13%), trans-limonene oxide (1.31%) and cislimonene oxide (1.21%). The obtained results suggest that the plant members of genus Citrus have an anthelmintic potential that origin from a high percentage of limonene, and may play important role in the future approaches designed for nematode control in animals. Further in vivo studies should be performed to confirm these findings.",
publisher = "Unique Scientific Publishers, Faisalabad, Pakistan",
journal = "Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan",
title = "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1970"
}
Štrbac, F., Bosco, A., Stojanović, D., Ratajac, R., Simin, N., Orčić, D., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan
Unique Scientific Publishers, Faisalabad, Pakistan., 27-27.
https://hdl.handle.net/21.15107/rcub_rimsi_1970
Štrbac F, Bosco A, Stojanović D, Ratajac R, Simin N, Orčić D, Petrović K, Cringoli G, Rinaldi L. In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes. in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan. 2022;:27-27.
https://hdl.handle.net/21.15107/rcub_rimsi_1970 .
Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Ratajac, Radomir, Simin, Nataša, Orčić, Dejan, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "In Vitro Anthelmintic Efficacy of Essential Oils of Citrus medica L. and Citrus sinensis L. against Sheep Gastrointestinal Nematodes" in Abstract Book of the International Conference on Remote Sensing and GIS Integration in Veterinary, Agricultural and Health Sciences, Multan, Pakistan (2022):27-27,
https://hdl.handle.net/21.15107/rcub_rimsi_1970 .

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