Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study
Authors
Štrbac, Filip
Bosco, Antonio
Stojanović, Dragica
Simin, Nataša

Orčić, Dejan
Ratajac, Radomir
Petrović, Kosta
Cringoli, Giuseppe
Rinaldi, Laura
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Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary
practice.
Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations
(50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo
Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated.
Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7%
dependi...ng on the concentration used. The high anthelmintic potential was confirmed in the FECRT
with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%).
Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep
GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance.
Keywords:
anthelmintic resistance / origanum essential oil / in vitro EHT / in vivo FECRT / gastrointestinal nematodesSource:
Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark, 2022Publisher:
- World Federation of Parasitologists
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Štrbac, Filip AU - Bosco, Antonio AU - Stojanović, Dragica AU - Simin, Nataša AU - Orčić, Dejan AU - Ratajac, Radomir AU - Petrović, Kosta AU - Cringoli, Giuseppe AU - Rinaldi, Laura PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1967 AB - Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary practice. Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated. Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7% depending on the concentration used. The high anthelmintic potential was confirmed in the FECRT with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%). Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance. PB - World Federation of Parasitologists C3 - Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark T1 - Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study UR - https://hdl.handle.net/21.15107/rcub_rimsi_1967 ER -
@conference{ author = "Štrbac, Filip and Bosco, Antonio and Stojanović, Dragica and Simin, Nataša and Orčić, Dejan and Ratajac, Radomir and Petrović, Kosta and Cringoli, Giuseppe and Rinaldi, Laura", year = "2022", abstract = "Introduction: The management of nematode infections in animals represents a challenge due to the development of anthelmintic resistance to commercial drugs. This study aimed to examine the anthelmintic efficacy of the Origanum vulgare (L.) essential oil (EO) against gastrointestinal nematodes (GINs) of sheep, as well as to evaluate the possibility of its application in veterinary practice. Methods: Firstly, the in vitro Egg Hatch Test (EHT) was conducted at eight different concentrations (50, 12.5, 3.125, 0.781, 0.195, 0.049, 0.025 and 0.0125 mg/mL) of the tested EO. Next, the in vivo Faecal Egg Count Reduction Test (FECRT) was performed using sheep from two farms in southern Italy, whereby the O. vulgare EO was administrated orally at a mean single dose of 150 mg/kg. The toxic effects of the applied EO on animals, as well as EO chemical composition, were also evaluated. Results: In the EHT, ovicidal activity, i.e. the inhibition of egg hatchability varied from 71.3-93.7% depending on the concentration used. The high anthelmintic potential was confirmed in the FECRT with a total reduction of nematode eggs in faeces of 43.2% and 60.1% at days 7 and 14 after treatment, respectively. In addition, no toxic effects were noticed during clinical examination of sheep or observing blood count and liver function tests results. The main compounds of the tested EO identified by GC-MS analyses were carvacrol (76.2%), p-cymene (12.6%) and γ-terpinene (2.6%). Conclusions: The obtained results suggest the strong activity of the O. vulgare EO against sheep GINs due to a high percentage of carvacrol, whereby it can be considered safe for sheep at the dose tested in vivo. Therefore, EO of oregano may be applied in veterinary practice in order to reduce the use of commercial drugs and combat anthelmintic resistance.", publisher = "World Federation of Parasitologists", journal = "Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark", title = "Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1967" }
Štrbac, F., Bosco, A., Stojanović, D., Simin, N., Orčić, D., Ratajac, R., Petrović, K., Cringoli, G.,& Rinaldi, L.. (2022). Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study. in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark World Federation of Parasitologists.. https://hdl.handle.net/21.15107/rcub_rimsi_1967
Štrbac F, Bosco A, Stojanović D, Simin N, Orčić D, Ratajac R, Petrović K, Cringoli G, Rinaldi L. Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study. in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark. 2022;. https://hdl.handle.net/21.15107/rcub_rimsi_1967 .
Štrbac, Filip, Bosco, Antonio, Stojanović, Dragica, Simin, Nataša, Orčić, Dejan, Ratajac, Radomir, Petrović, Kosta, Cringoli, Giuseppe, Rinaldi, Laura, "Anthelmintic Properties of Origanum vulgare (L.) Essential Oil against Gastrointestinal Nematodes of Sheep - In Vitro, In Vivo and Toxicity Study" in Book of Abstracts of 15th International Congress of Parasitology (ICOPA 2022), Copenhagen, Denmark (2022), https://hdl.handle.net/21.15107/rcub_rimsi_1967 .