First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia
Abstract
In August 2021, symptoms of soft rot appeared on cabbage
in Futog (Bačka, Vojvodina) locality known for traditional
cabbage cultivation in Serbia. Symptoms appeared in the
form of sunken and soft lesions on outer head leaves, while
interior tissue was macerated, with cream to black
discoloration. A strong, specific odor followed the
breakdown of the affected tissue. From the 1 ha field, a
total of 5 heads were collected for the isolation of causing
pathogen. Margins of healthy and dead tissues were
macerated and plated on Crystal Violet Pectate medium.
Five creamy-white colonies forming pits in the medium
were purified. These bacterial isolates were positive for
pectinolytic activity on cabbage heads and potato slices.
They were all facultative anaerobic, Gram-negative,
catalase-positive, and oxidase-negative; unable to produce
diffusible fluorescent pigments. Multilocus sequence
typing of cabbage isolates was performed by sequencing
of four housekeepi...ng genes (proA, dnaX, icdA, mdh).
Based on the nucleotide BLAST (National Center for
Biotechnology Information, NCBI) analysis of the
sequences of genes icdA and mdh, Serbian cabbage isolates
were 100% identical with Pectobacterium carotovorum
subsp. odoriferum strain BC S7. Based on the dnaX gene,
identity with the same strain was 99.79% and 99.57%
based on the proA gene. Serbian cabbage isolates also
showed 100% identity with other NCBI deposited P.
odoriferum strains CFBP1878 and ATCC 25272 based on
the icdA gene, as well as ICMP 11533 based on mdh gene.
Therefore, this first report of P. odoriferum indicates a
wider range of Pectobacterium spp. recently described in
Serbia.
Keywords:
soft rot / identification / PectobacteriumSource:
14th International Conference on Plant Pathogenic Bacteria, 2022, 123-Publisher:
- Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-200010)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200032 (Institute of Field and Vegetable Crops, Novi Sad) (RS-200032)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200117 (University of Novi Sad, Faculty of Agriculture) (RS-200117)
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Marković, Sanja AU - Popović, Tatjana AU - Mitrović, Petar AU - Iličić, Renata AU - Jelušić, Aleksandra PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1959 AB - In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia. PB - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini C3 - 14th International Conference on Plant Pathogenic Bacteria T1 - First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia SP - 123 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1959 ER -
@conference{ author = "Marković, Sanja and Popović, Tatjana and Mitrović, Petar and Iličić, Renata and Jelušić, Aleksandra", year = "2022", abstract = "In August 2021, symptoms of soft rot appeared on cabbage in Futog (Bačka, Vojvodina) locality known for traditional cabbage cultivation in Serbia. Symptoms appeared in the form of sunken and soft lesions on outer head leaves, while interior tissue was macerated, with cream to black discoloration. A strong, specific odor followed the breakdown of the affected tissue. From the 1 ha field, a total of 5 heads were collected for the isolation of causing pathogen. Margins of healthy and dead tissues were macerated and plated on Crystal Violet Pectate medium. Five creamy-white colonies forming pits in the medium were purified. These bacterial isolates were positive for pectinolytic activity on cabbage heads and potato slices. They were all facultative anaerobic, Gram-negative, catalase-positive, and oxidase-negative; unable to produce diffusible fluorescent pigments. Multilocus sequence typing of cabbage isolates was performed by sequencing of four housekeeping genes (proA, dnaX, icdA, mdh). Based on the nucleotide BLAST (National Center for Biotechnology Information, NCBI) analysis of the sequences of genes icdA and mdh, Serbian cabbage isolates were 100% identical with Pectobacterium carotovorum subsp. odoriferum strain BC S7. Based on the dnaX gene, identity with the same strain was 99.79% and 99.57% based on the proA gene. Serbian cabbage isolates also showed 100% identity with other NCBI deposited P. odoriferum strains CFBP1878 and ATCC 25272 based on the icdA gene, as well as ICMP 11533 based on mdh gene. Therefore, this first report of P. odoriferum indicates a wider range of Pectobacterium spp. recently described in Serbia.", publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini", journal = "14th International Conference on Plant Pathogenic Bacteria", title = "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia", pages = "123", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1959" }
Marković, S., Popović, T., Mitrović, P., Iličić, R.,& Jelušić, A.. (2022). First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia. in 14th International Conference on Plant Pathogenic Bacteria Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini., 123. https://hdl.handle.net/21.15107/rcub_rimsi_1959
Marković S, Popović T, Mitrović P, Iličić R, Jelušić A. First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia. in 14th International Conference on Plant Pathogenic Bacteria. 2022;:123. https://hdl.handle.net/21.15107/rcub_rimsi_1959 .
Marković, Sanja, Popović, Tatjana, Mitrović, Petar, Iličić, Renata, Jelušić, Aleksandra, "First report of Pectobacterium odoriferum causing bacterial soft rot of cabbage in Serbia" in 14th International Conference on Plant Pathogenic Bacteria (2022):123, https://hdl.handle.net/21.15107/rcub_rimsi_1959 .