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Pseudomonas savastanoi pv. glycinea affecting a vegetable soybean for commercial edamame production in Serbia

Authorized Users Only
2022
Authors
Popović, Tatjana
Jelušić, Aleksandra
Aćimović, Radivoje
Marković, Sanja
Iličić, Renata
Conference object (Published version)
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Abstract
The vegetable soybean “edamame” (Glicine max L.) is a nutritious legume having pods and seeds that can be harvested and consumed while they are still fresh and premature. Edamame is rich in micronutrients and vitamins and is therefore used as food and for medicinal purposes. In Serbia, edamame production started for the first time in 2019 on a 10 ha field in Bečej locality (Bačka, Vojvodina). Later, in 2021, its production was expanded to 30 ha, but in summer was followed with symptoms of bacterial leaf spot. The symptoms appeared on leaves in the form of water-soaked spots surrounded by a chlorotic halo that enlarged and coalesced into necrotic lesions. Disease incidence was from 15-20%. Isolation of the causal pathogen was performed by sowing of suspension of the macerated margins of spots/lesions from ten collected symptomatic leaves on nutrient agar supplemented with 5% sucrose. Ten representative, purified isolates were whitish, circular, smooth, ...shiny, levan-positive, strictly aerobic, gram-negative; positive for green-fluorescent pigment and tobacco hypersensitive response, and negative for oxidase, arginine dihydrolase, and potato soft rot (LOPAT group Ia). Pathogenicity of the isolates was confirmed on soybean cotyledons by under pressure atomizing a bacterial suspension (107-8 CFU mL 1 ). Sequencing of genes gapA, gyrB, and rpoD showed 100% homology of the obtained isolates with Pseudomonas savastanoi pv. glycinea pathotype strain LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, MOC601, R4a (gapA and gyrB), and M301765 (gapA and rpoD) originated from soybean, all from the Plant Associated and Environmental Microbes Database (PAMDB).

Keywords:
disease incidence / identification / PAMDB
Source:
14th International Conference on Plant Pathogenic Bacteria, 2022, 124-
Publisher:
  • Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria Progetto grafico - editoriale esecutivo, Diletta Pini
Funding / projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-200010)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200117 (University of Novi Sad, Faculty of Agriculture) (RS-200117)
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rimsi_1958
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1958
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - CONF
AU  - Popović, Tatjana
AU  - Jelušić, Aleksandra
AU  - Aćimović, Radivoje
AU  - Marković, Sanja
AU  - Iličić, Renata
PY  - 2022
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1958
AB  - The vegetable soybean “edamame” (Glicine max L.) is a 
nutritious legume having pods and seeds that can be 
harvested and consumed while they are still fresh and 
premature. Edamame is rich in micronutrients and 
vitamins and is therefore used as food and for medicinal 
purposes. In Serbia, edamame production started for the 
first time in 2019 on a 10 ha field in Bečej locality (Bačka, 
Vojvodina). Later, in 2021, its production was expanded 
to 30 ha, but in summer was followed with symptoms of 
bacterial leaf spot. The symptoms appeared on leaves in 
the form of water-soaked spots surrounded by a chlorotic 
halo that enlarged and coalesced into necrotic lesions. 
Disease incidence was from 15-20%. Isolation of the 
causal pathogen was performed by sowing of suspension 
of the macerated margins of spots/lesions from ten 
collected symptomatic leaves on nutrient agar 
supplemented with 5% sucrose. Ten representative, 
purified isolates were whitish, circular, smooth, shiny, 
levan-positive, strictly aerobic, gram-negative; positive for 
green-fluorescent pigment and tobacco hypersensitive 
response, and negative for oxidase, arginine dihydrolase, 
and potato soft rot (LOPAT group Ia). Pathogenicity of the 
isolates was confirmed on soybean cotyledons by under 
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed 
100% homology of the obtained isolates with 
Pseudomonas savastanoi pv. glycinea pathotype strain 
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, 
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant 
Associated and Environmental Microbes Database 
(PAMDB).
PB  - Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini
C3  - 14th International Conference   on Plant Pathogenic Bacteria
T1  - Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia
SP  - 124
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1958
ER  - 
@conference{
author = "Popović, Tatjana and Jelušić, Aleksandra and Aćimović, Radivoje and Marković, Sanja and Iličić, Renata",
year = "2022",
abstract = "The vegetable soybean “edamame” (Glicine max L.) is a 
nutritious legume having pods and seeds that can be 
harvested and consumed while they are still fresh and 
premature. Edamame is rich in micronutrients and 
vitamins and is therefore used as food and for medicinal 
purposes. In Serbia, edamame production started for the 
first time in 2019 on a 10 ha field in Bečej locality (Bačka, 
Vojvodina). Later, in 2021, its production was expanded 
to 30 ha, but in summer was followed with symptoms of 
bacterial leaf spot. The symptoms appeared on leaves in 
the form of water-soaked spots surrounded by a chlorotic 
halo that enlarged and coalesced into necrotic lesions. 
Disease incidence was from 15-20%. Isolation of the 
causal pathogen was performed by sowing of suspension 
of the macerated margins of spots/lesions from ten 
collected symptomatic leaves on nutrient agar 
supplemented with 5% sucrose. Ten representative, 
purified isolates were whitish, circular, smooth, shiny, 
levan-positive, strictly aerobic, gram-negative; positive for 
green-fluorescent pigment and tobacco hypersensitive 
response, and negative for oxidase, arginine dihydrolase, 
and potato soft rot (LOPAT group Ia). Pathogenicity of the 
isolates was confirmed on soybean cotyledons by under 
pressure atomizing a bacterial suspension (107-8 CFU mL 1
). Sequencing of genes gapA, gyrB, and rpoD showed 
100% homology of the obtained isolates with 
Pseudomonas savastanoi pv. glycinea pathotype strain 
LMG 5066 and strains BR1, KN166, KN28, KN44, LN10, 
MOC601, R4a (gapA and gyrB), and M301765 (gapA and
rpoD) originated from soybean, all from the Plant 
Associated and Environmental Microbes Database 
(PAMDB).",
publisher = "Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini",
journal = "14th International Conference   on Plant Pathogenic Bacteria",
title = "Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia",
pages = "124",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1958"
}
Popović, T., Jelušić, A., Aćimović, R., Marković, S.,& Iličić, R.. (2022). Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria
Impaginazione e stampa a cura del Centro Stampa – Giunta Regionale – Regione Umbria  Progetto grafico - editoriale esecutivo, Diletta Pini., 124.
https://hdl.handle.net/21.15107/rcub_rimsi_1958
Popović T, Jelušić A, Aćimović R, Marković S, Iličić R. Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia. in 14th International Conference   on Plant Pathogenic Bacteria. 2022;:124.
https://hdl.handle.net/21.15107/rcub_rimsi_1958 .
Popović, Tatjana, Jelušić, Aleksandra, Aćimović, Radivoje, Marković, Sanja, Iličić, Renata, "Pseudomonas savastanoi pv. glycinea affecting a  vegetable soybean for commercial edamame  production in Serbia" in 14th International Conference   on Plant Pathogenic Bacteria (2022):124,
https://hdl.handle.net/21.15107/rcub_rimsi_1958 .

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