IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA
Authors
Jelušić, Aleksandra
Berić, Tanja
Dimkić, Ivica
Mitrović, Petar
Stanković, Slaviša

Marković, Sanja

Popović, Tatjana

Conference object (Published version)

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Background:
Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium
Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as
a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression.
Objectives:
With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia,
this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like
isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants.
Methods:
Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free
supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winte...r oilseed rape
Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the
sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced.
Results:
Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B.
cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B.
megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.
Keywords:
antagonism / Bacillus / PseudomonasSource:
FEMS Online Conference on Microbiology, 2020, 133-Publisher:
- Federation of European Microbiological Societies in association with the Serbian Society of Microbiology
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Jelušić, Aleksandra AU - Berić, Tanja AU - Dimkić, Ivica AU - Mitrović, Petar AU - Stanković, Slaviša AU - Marković, Sanja AU - Popović, Tatjana PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1950 AB - Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant. PB - Federation of European Microbiological Societies in association with the Serbian Society of Microbiology C3 - FEMS Online Conference on Microbiology T1 - IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA SP - 133 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1950 ER -
@conference{ author = "Jelušić, Aleksandra and Berić, Tanja and Dimkić, Ivica and Mitrović, Petar and Stanković, Slaviša and Marković, Sanja and Popović, Tatjana", year = "2020", abstract = "Background: Yield and quality of oilseed rape and other Brassicacceae vegetables are affected with plant pathogenic bacterium Xanthomonas campestris pv. campestris (Xcc), both worldwide and in Serbia. Considering the losses that can arise as a consequence of the infection with this pathogen, there is an increasing need for finding the effective and environ mentally safe method for its suppression. Objectives: With the aim to find a promising biological control agent for managing Xcc infection on winter oilseed rape in Serbia, this research was based on evaluation of the in vitro antagonistic potential of the Bacillus- and Pseudomonas-like isolates obtained from the phyllosphere and rhizosphere of the diseased and healthy winter oilseed rape plants. Methods: Well diffusion technique was used to evaluate the potential inhibitory activity of the whole cultures and cell-free supernatants of the 36 Bacillus- and Pseudomonas-like isolates, against the collection of Serbian winter oilseed rape Xcc isolates. DNA of the isolates which were able to inhibit Xcc growth was amplified with the primers based on the sequences of tuf and gyrB genes for Bacillus- and Pseudomonas-like isolates, respectively and sequenced. Results: Nine Bacillus spp. isolates whose whole cultures showed antagonistic potential were identified as B. thuringiensis/B. cereus (5); B. valezensis (1); B. mobilis (1); B. megaterium (1) and B. halotolerans (1), while six Pseudomonas spp. iso lates were identified as P. orientalis (4) and P. fluorescens (2). Only five isolates within three species (B. valezensis, B. megaterium and P. orientalis) had both, the activity of the whole culture and supernatant.", publisher = "Federation of European Microbiological Societies in association with the Serbian Society of Microbiology", journal = "FEMS Online Conference on Microbiology", title = "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA", pages = "133", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1950" }
Jelušić, A., Berić, T., Dimkić, I., Mitrović, P., Stanković, S., Marković, S.,& Popović, T.. (2020). IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology Federation of European Microbiological Societies in association with the Serbian Society of Microbiology., 133. https://hdl.handle.net/21.15107/rcub_rimsi_1950
Jelušić A, Berić T, Dimkić I, Mitrović P, Stanković S, Marković S, Popović T. IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA. in FEMS Online Conference on Microbiology. 2020;:133. https://hdl.handle.net/21.15107/rcub_rimsi_1950 .
Jelušić, Aleksandra, Berić, Tanja, Dimkić, Ivica, Mitrović, Petar, Stanković, Slaviša, Marković, Sanja, Popović, Tatjana, "IN VITRO ASSESSMENT OF THE ANTAGONISTIC POTENTIAL OF BACILLUS SPP. AND PSEUDOMONAS SPP. AGAINST XANTHOMONAS CAMPESTRIS PV. CAMPESTRIS ISOLATED FROM WINTER OILSEED RAPE IN SERBIA" in FEMS Online Conference on Microbiology (2020):133, https://hdl.handle.net/21.15107/rcub_rimsi_1950 .