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Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape

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Authors
Jelušić, Aleksandra
Dimkić, Ivica
Berić, Tanja
Mitrović, Petar
Marković, Sanja
Stanković, Slaviša
Popović, Tatjana
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Abstract
Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae vegetables. Objectives: Metagenomic approach was used to reveal differences in microbial communities colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial blight symptoms and one healthy variety, all collected from five different localities in Vojvodina (Serbia) in 2018. Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA sequences from five tested samples, and to gain insight into their alpha and beta diversity. Comparative taxonomic analysis of the obtained sequencing metadata was processed bioi...nformaticaly. Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) in healthy oilseed rape variety.

Keywords:
Xanthomonas campestris pv. campestris / Brassica napus / Next generation sequencing
Source:
8th Congress of European Microbiologists, 2019, 899-
Publisher:
  • Federation of European Microbiological Societies
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rimsi_1935
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1935
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Dimkić, Ivica
AU  - Berić, Tanja
AU  - Mitrović, Petar
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1935
AB  - Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape
SP  - 899
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1935
ER  - 
@conference{
author = "Jelušić, Aleksandra and Dimkić, Ivica and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana",
year = "2019",
abstract = "Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or 
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. 
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight 
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities 
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina 
(Serbia) in 2018. 
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity. 
Comparative taxonomic analysis of the obtained sequencing metadata was processed 
bioinformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, 
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. 
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike 
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the 
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were 
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in 
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) 
in healthy oilseed rape variety.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape",
pages = "899",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1935"
}
Jelušić, A., Dimkić, I., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935
Jelušić A, Dimkić I, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape. in 8th Congress of  European Microbiologists. 2019;:899.
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .
Jelušić, Aleksandra, Dimkić, Ivica, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the  diseased and healthy oilseed rape" in 8th Congress of  European Microbiologists (2019):899,
https://hdl.handle.net/21.15107/rcub_rimsi_1935 .

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