Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape
Authors
Jelušić, Aleksandra
Dimkić, Ivica
Berić, Tanja
Mitrović, Petar
Marković, Sanja

Stanković, Slaviša
Popović, Tatjana

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Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or
endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere.
Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight
symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae
vegetables.
Objectives: Metagenomic approach was used to reveal differences in microbial communities
colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial
blight symptoms and one healthy variety, all collected from five different localities in Vojvodina
(Serbia) in 2018.
Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA
sequences from five tested samples, and to gain insight into their alpha and beta diversity.
Comparative taxonomic analysis of the obtained sequencing metadata was processed
bioi...nformaticaly.
Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria,
Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes.
Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike
healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the
most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were
present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in
healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then
1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae)
in healthy oilseed rape variety.
Keywords:
Xanthomonas campestris pv. campestris / Brassica napus / Next generation sequencingSource:
8th Congress of European Microbiologists, 2019, 899-Publisher:
- Federation of European Microbiological Societies
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Jelušić, Aleksandra AU - Dimkić, Ivica AU - Berić, Tanja AU - Mitrović, Petar AU - Marković, Sanja AU - Stanković, Slaviša AU - Popović, Tatjana PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1935 AB - Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae vegetables. Objectives: Metagenomic approach was used to reveal differences in microbial communities colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial blight symptoms and one healthy variety, all collected from five different localities in Vojvodina (Serbia) in 2018. Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA sequences from five tested samples, and to gain insight into their alpha and beta diversity. Comparative taxonomic analysis of the obtained sequencing metadata was processed bioinformaticaly. Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) in healthy oilseed rape variety. PB - Federation of European Microbiological Societies C3 - 8th Congress of European Microbiologists T1 - Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape SP - 899 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1935 ER -
@conference{ author = "Jelušić, Aleksandra and Dimkić, Ivica and Berić, Tanja and Mitrović, Petar and Marković, Sanja and Stanković, Slaviša and Popović, Tatjana", year = "2019", abstract = "Background: A wide variety of different saprophytic and pathogenic bactera, both living epiphytic or endophytic, colonize physically and nutritionally different microhabitats on plants phyllosphere. Xanthomonas campestris pv. campestris (Xcc) is phytopathogenic bacteria causing bacterial blight symptom on oilseed rape (Brassica napus) and black rot on other cultivated Brassica oleaceae vegetables. Objectives: Metagenomic approach was used to reveal differences in microbial communities colonizing the phyllosphere of four different winter oilseed rape varieties with expressed bacterial blight symptoms and one healthy variety, all collected from five different localities in Vojvodina (Serbia) in 2018. Methods: Next generation sequencing (NGS) Illumina MiSeq platform was used to obtain 16S rRNA sequences from five tested samples, and to gain insight into their alpha and beta diversity. Comparative taxonomic analysis of the obtained sequencing metadata was processed bioinformaticaly. Results: Different percetages of bacterial species belonging to three bacterial phyla-Proteobacteria, Firmicutes, and Actinobacteria were dominant in phyllosphere of all five oilseed rape varietes. Bacteria from the phylum Proteobacteria (66-91%) were dominant in diseased varieties, unlike healthy variety where only 38% belonged to this phylum. Genus Xanthomonas (40-74%) was the most abundant in diseased plants phyllosphere. Only 0.2% of Xanthomonas representatives were present in healthy plants, where order Enterobacteriales (Pantoea, Buchnera, and ud Enterobacteriaceae) were the most abundant. The genus Exiguobacterium was the most abundant in healthy variety (47%). Bacteria from phylum Acintinobacteria were least represented, with less then 1% in diseased and up to 12% (ud-Micrococcaceae, Kocuria, Arthrobacter, and ud-Microbacteriaceae) in healthy oilseed rape variety.", publisher = "Federation of European Microbiological Societies", journal = "8th Congress of European Microbiologists", title = "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape", pages = "899", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1935" }
Jelušić, A., Dimkić, I., Berić, T., Mitrović, P., Marković, S., Stanković, S.,& Popović, T.. (2019). Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape. in 8th Congress of European Microbiologists Federation of European Microbiological Societies., 899. https://hdl.handle.net/21.15107/rcub_rimsi_1935
Jelušić A, Dimkić I, Berić T, Mitrović P, Marković S, Stanković S, Popović T. Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape. in 8th Congress of European Microbiologists. 2019;:899. https://hdl.handle.net/21.15107/rcub_rimsi_1935 .
Jelušić, Aleksandra, Dimkić, Ivica, Berić, Tanja, Mitrović, Petar, Marković, Sanja, Stanković, Slaviša, Popović, Tatjana, "Comparative metagenomics of microbial communities inhabiting the phyllosphere of the diseased and healthy oilseed rape" in 8th Congress of European Microbiologists (2019):899, https://hdl.handle.net/21.15107/rcub_rimsi_1935 .