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Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated from oilseed rape determined with different rep-PCR techniques

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2019
bitstream_5041.pdf (1.018Mb)
Authors
Jelušić, Aleksandra
Popović, Tatjana
Mitrović, Petar
Dimkić, Ivica
Stanković, Slaviša
Marković, Sanja
Berić, Tanja
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Abstract
Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape in Serbia. Ever since, it continued appearing on cultivated crops too. Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) fingerprinting. Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of genet...ic heterogeneity among Xcc isolates. Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were detected. These results show presence of genetic polymorphism among Xcc isolates from winter oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or formation of a new Xcc ecotype.

Keywords:
bacterial leaf blight / rep-PCR / Brassica napus
Source:
8th Congress of European Microbiologists, 2019, 991-
Publisher:
  • Federation of European Microbiological Societies
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rimsi_1933
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1933
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - CONF
AU  - Jelušić, Aleksandra
AU  - Popović, Tatjana
AU  - Mitrović, Petar
AU  - Dimkić, Ivica
AU  - Stanković, Slaviša
AU  - Marković, Sanja
AU  - Berić, Tanja
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1933
AB  - Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.
PB  - Federation of European Microbiological Societies
C3  - 8th Congress of  European Microbiologists
T1  - Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques
SP  - 991
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1933
ER  - 
@conference{
author = "Jelušić, Aleksandra and Popović, Tatjana and Mitrović, Petar and Dimkić, Ivica and Stanković, Slaviša and Marković, Sanja and Berić, Tanja",
year = "2019",
abstract = "Background: Oilseed rape (Brassica napus) is an oil-producing crop, significant for vegetable oil and 
biodiesel production. The phytopathogenic bacterium Xanthomonas campestris pv. campestris (Xcc) 
causing bacterial leaf blight was recorded for a first time in 2010 on self-growing winter oilseed rape 
in Serbia. Ever since, it continued appearing on cultivated crops too. 
Objectives: The aim of this research was to reveal genetic heterogeneity among 25 Xcc isolates, 
originated from different cultivars of winter oilseed rape obtained in nine-year period (2010-2018) 
from different localities in Vojvodina region (Serbia) using repetitive sequence-based PCR (rep-PCR) 
fingerprinting. 
Methods: Genomic DNA was extracted using CTAB method. Specific primer pair XCF/XCR, amplifying 
a DNA fragment of hrpF gene from X. campestris was used for rapid preliminary identification of all 
tested isolates. Rep-PCR using BOX, ERIC and REP-PCR primers was used to detect presence of 
genetic heterogeneity among Xcc isolates.
Results: All 25 tested isolates were preliminary identified as Xcc based on a positive PCR reaction 
after amplification of 525 bp region using XCF/XCR primers. BOX and ERIC-PCR generated seventeen 
different DNA fingerprinting profiles, unlike REP-PCR where only nine different profiles were 
detected. These results show presence of genetic polymorphism among Xcc isolates from winter 
oilseed rape, which could indicate either their affiliation to some of the identified Xcc races, or 
formation of a new Xcc ecotype.",
publisher = "Federation of European Microbiological Societies",
journal = "8th Congress of  European Microbiologists",
title = "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques",
pages = "991",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1933"
}
Jelušić, A., Popović, T., Mitrović, P., Dimkić, I., Stanković, S., Marković, S.,& Berić, T.. (2019). Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists
Federation of European Microbiological Societies., 991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933
Jelušić A, Popović T, Mitrović P, Dimkić I, Stanković S, Marković S, Berić T. Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques. in 8th Congress of  European Microbiologists. 2019;:991.
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .
Jelušić, Aleksandra, Popović, Tatjana, Mitrović, Petar, Dimkić, Ivica, Stanković, Slaviša, Marković, Sanja, Berić, Tanja, "Genetic heterogeneity among Xanthomonas campestris pv. campestris isolates originated  from oilseed rape determined with different rep-PCR techniques" in 8th Congress of  European Microbiologists (2019):991,
https://hdl.handle.net/21.15107/rcub_rimsi_1933 .

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