Metagenomic analysis of microbial communities associated with diseased potato tubers
Authors
Marković, Sanja
Dimkić, Ivica
Stanković, Slaviša
Jelušić, Aleksandra
Iličić, Renata
Popović, Tatjana

Conference object (Published version)

Metadata
Show full item recordAbstract
Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and
soft rot are commercially important pathogens in potato production. Blackleg symptom caused by
these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in
2018.
Objectives: Metagenomic approach was used to reveal microbial communities colonizing the
diseased potato tubers with the aim of better understanding of pathogens distribution.
Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and
checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the
species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from
diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina
platform. The obtained sequencing data was processed bioinformaticaly.
Results: Mixed infection of the diseased potato pl...ants with Dickeya dianthocola and Pectobacterium
carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All
isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present
bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and
ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and
ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and
Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%.
Keywords:
Dickeya / Pectobacterium / blacklegSource:
8th Congress of European Microbiologists, 2019, 881-Publisher:
- Federation of European Microbiological Societies
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Marković, Sanja AU - Dimkić, Ivica AU - Stanković, Slaviša AU - Jelušić, Aleksandra AU - Iličić, Renata AU - Popović, Tatjana PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1931 AB - Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and soft rot are commercially important pathogens in potato production. Blackleg symptom caused by these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in 2018. Objectives: Metagenomic approach was used to reveal microbial communities colonizing the diseased potato tubers with the aim of better understanding of pathogens distribution. Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina platform. The obtained sequencing data was processed bioinformaticaly. Results: Mixed infection of the diseased potato plants with Dickeya dianthocola and Pectobacterium carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%. PB - Federation of European Microbiological Societies C3 - 8th Congress of European Microbiologists T1 - Metagenomic analysis of microbial communities associated with diseased potato tubers SP - 881 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1931 ER -
@conference{ author = "Marković, Sanja and Dimkić, Ivica and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana", year = "2019", abstract = "Background: Pectolytic Dickeya and Pectobacterium species that cause blackleg, aerial stem rot and soft rot are commercially important pathogens in potato production. Blackleg symptom caused by these bacteria has been recorded in potato production field in Bačka region (Vojvodina, Serbia) in 2018. Objectives: Metagenomic approach was used to reveal microbial communities colonizing the diseased potato tubers with the aim of better understanding of pathogens distribution. Methods: Single colonies of potential pathogenic bacteria were isolated on nutrient agar and checked for pectolytic activity on potato slices. Ten selected bacterial isolates were identified to the species level after PCR amplification with gapA and mdh primers. The 16S rRNA sequences from diseased potato tubers samples were obtained using Next generation sequencing (NGS) Illumina platform. The obtained sequencing data was processed bioinformaticaly. Results: Mixed infection of the diseased potato plants with Dickeya dianthocola and Pectobacterium carotovorum pv. brasiliensis was revealed after pathogen identification with gapA and mdh genes. All isolates caused soft rot symptoms on potato slices. Metagenomic analysis revealed that present bacteria belong to phylum Bacteroidetes (Bacteroides, Empedobacter, Dysgonomonas, Myroides, and ud-Porphyromonadaceae), Firmicutes (Vagococcus, Lactococcus, Clostridium XIVa, Enterococcus, and ud-Acidaminococcaceae) and Proteobacteria (Acinetobacter, ud-Pseudomonadaceae, ud Enterobacteriaceae, Wohlfahrtiimonas, ud-Neisseriaceae, Enterobacter, Providencia, Arcobacter, and Kerstersia) as the most common representives. Genus Pectobacterium was present in 0.5%.", publisher = "Federation of European Microbiological Societies", journal = "8th Congress of European Microbiologists", title = "Metagenomic analysis of microbial communities associated with diseased potato tubers", pages = "881", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1931" }
Marković, S., Dimkić, I., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2019). Metagenomic analysis of microbial communities associated with diseased potato tubers. in 8th Congress of European Microbiologists Federation of European Microbiological Societies., 881. https://hdl.handle.net/21.15107/rcub_rimsi_1931
Marković S, Dimkić I, Stanković S, Jelušić A, Iličić R, Popović T. Metagenomic analysis of microbial communities associated with diseased potato tubers. in 8th Congress of European Microbiologists. 2019;:881. https://hdl.handle.net/21.15107/rcub_rimsi_1931 .
Marković, Sanja, Dimkić, Ivica, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "Metagenomic analysis of microbial communities associated with diseased potato tubers" in 8th Congress of European Microbiologists (2019):881, https://hdl.handle.net/21.15107/rcub_rimsi_1931 .