dc.description.abstract | Recent studies have been made to investigate some molecular features of plant pathogenic
bacteria Pseudomonas syringae pv. coriandricola. This pathogen causes bacterial leaf spot
disease on some members of family Apiaceae, such as coriander, carrot, parsnip and parsley.
Initial symptoms are small water-soaked lesions on foliage, which then develop into spots
varying in shades, tan, brown or black. The spots are usually limited by leaf veins having an
angular appearance, and visible from both sides of leaves. Under favorable conditions of high
humidity, spots may spread and cause foliage blighting. P. s. pv. coriandricola strains used in this
study originate from carrot, parsley and parsnip. Repetitive element PCR fingerprinting (rep PCR), using REP, BOX, ERIC, (GTG)5 and SERE primers, and randomly amplified polymorphic
DNA-polymerase chain reaction (RAPD-PCR) using M13 primer were used to determinate
genetic polymorphism between tested strains. Further molecular features were observed on the
basis of a multilocus sequence analysis (MLSA) by sequencing key genes for Pseudomonas
syringae (gapA, gltA, gyrB, rpoD). DNA profiling revealed identical banding patterns for all
tested strains. The sequence and phylogenetic analysis of three MLSA genes (gapA, gyrB, rpoD)
showed homology with pathotype strains P. s. pv. coriandricola deposited in PAMDB
(http://genome.ppws.vt.edu/cgi-bin/MLST/home.pl). Sequencing of gltA gene determined the
isolates above the species level (P. syringae). Our results indicate that gapA, gyrB and rpoD
genes are more discriminatory then gltA and their usage is therefore suggested for further
genotypic studies of this pathogen. | sr |