MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM
2018
Преузимање 🢃
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
BACKGROUNDS: Ralstonia solanacearum race 3 biovar 2 is one of the most important plant
pathogenic bacteria because of its persistence, wide host range and widespread. It´s causing
bacterial wilt of over 250 plant species, including many cultivated crops (potato, tomato, eggplant,
geranium, ginger, banana, etc.) and brown rot of potato.
OBJECTIVES: The aim of this study was to determine presence of R. solanacearum based on
several years monitoring of potato tubers.
METHODS: During four-year period (2013-2016), 98 samples of potato tubers (11 cultivars),
from 13 localities in Bačka and 1 in Podunavlje region were examined. Isolation from potato
tubers with brown rot symptom was performed on semi-selective SMSA medium. PCR method
using Ps-1/Ps-2 and OLY1/Y2 primers and immunofluorescence were used to detect presence of
R. solanacearum. Pathogenicity was tested on young tomato plants.
RESULTS: In 2013, R. solanacearum was detected in 1 sample of potato tuber (Lady Claire...) from
Srpski Miletić; in 2015 in 2 (Crisps4all, VR 808) from Srpski Miletić and 1 (VR 808) from Stapar;
during 2016 in 2 (Crisps4all, Brooke) from Srpski Miletić, 2 (Lady Claire, VR 808) from Stapar, 2
(Lady Claire, Pirol) from Sombor and 1 (Panda) from Boleč. Milky-white, with pink to red centre,
flat, irregular, fluidal colonies formed after 3-4 days of incubation on SMSA medium. A 553 bp and
288 bp fragments of 65 representative isolates were amplified using Ps-1/Ps-2 and OLY1/Y2
primer pairs, respectively. IF test proved presence of fluorescent bacterial cells. All strains caused
wilting on tomato seedlings four days after inoculation.
CONCLUSIONS: The results of this study detected presence of R. solanacearum on 11 potato
samples isolated from four locations during period from 2013-2016.
Кључне речи:
Ralstonia solanacearum / potato / brown rotИзвор:
12TH CONGRESS OF SERBIAN MICROBIOLOGISTS with international participation MICROMED 2018 REGIO, 2018, 209-210Издавач:
- Udruženje mikrobiologa Srbije
Финансирање / пројекти:
- This study was funded by the Ministry of Education, Science and Technological Development of Serbia, Projects III43010 and OI173026.
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Marković, Sanja AU - Popović, Tatjana AU - Stanković, Slaviša AU - Jelušić, Aleksandra PY - 2018 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1928 AB - BACKGROUNDS: Ralstonia solanacearum race 3 biovar 2 is one of the most important plant pathogenic bacteria because of its persistence, wide host range and widespread. It´s causing bacterial wilt of over 250 plant species, including many cultivated crops (potato, tomato, eggplant, geranium, ginger, banana, etc.) and brown rot of potato. OBJECTIVES: The aim of this study was to determine presence of R. solanacearum based on several years monitoring of potato tubers. METHODS: During four-year period (2013-2016), 98 samples of potato tubers (11 cultivars), from 13 localities in Bačka and 1 in Podunavlje region were examined. Isolation from potato tubers with brown rot symptom was performed on semi-selective SMSA medium. PCR method using Ps-1/Ps-2 and OLY1/Y2 primers and immunofluorescence were used to detect presence of R. solanacearum. Pathogenicity was tested on young tomato plants. RESULTS: In 2013, R. solanacearum was detected in 1 sample of potato tuber (Lady Claire) from Srpski Miletić; in 2015 in 2 (Crisps4all, VR 808) from Srpski Miletić and 1 (VR 808) from Stapar; during 2016 in 2 (Crisps4all, Brooke) from Srpski Miletić, 2 (Lady Claire, VR 808) from Stapar, 2 (Lady Claire, Pirol) from Sombor and 1 (Panda) from Boleč. Milky-white, with pink to red centre, flat, irregular, fluidal colonies formed after 3-4 days of incubation on SMSA medium. A 553 bp and 288 bp fragments of 65 representative isolates were amplified using Ps-1/Ps-2 and OLY1/Y2 primer pairs, respectively. IF test proved presence of fluorescent bacterial cells. All strains caused wilting on tomato seedlings four days after inoculation. CONCLUSIONS: The results of this study detected presence of R. solanacearum on 11 potato samples isolated from four locations during period from 2013-2016. PB - Udruženje mikrobiologa Srbije C3 - 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS with international participation MICROMED 2018 REGIO T1 - MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM EP - 210 SP - 209 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1928 ER -
@conference{ author = "Marković, Sanja and Popović, Tatjana and Stanković, Slaviša and Jelušić, Aleksandra", year = "2018", abstract = "BACKGROUNDS: Ralstonia solanacearum race 3 biovar 2 is one of the most important plant pathogenic bacteria because of its persistence, wide host range and widespread. It´s causing bacterial wilt of over 250 plant species, including many cultivated crops (potato, tomato, eggplant, geranium, ginger, banana, etc.) and brown rot of potato. OBJECTIVES: The aim of this study was to determine presence of R. solanacearum based on several years monitoring of potato tubers. METHODS: During four-year period (2013-2016), 98 samples of potato tubers (11 cultivars), from 13 localities in Bačka and 1 in Podunavlje region were examined. Isolation from potato tubers with brown rot symptom was performed on semi-selective SMSA medium. PCR method using Ps-1/Ps-2 and OLY1/Y2 primers and immunofluorescence were used to detect presence of R. solanacearum. Pathogenicity was tested on young tomato plants. RESULTS: In 2013, R. solanacearum was detected in 1 sample of potato tuber (Lady Claire) from Srpski Miletić; in 2015 in 2 (Crisps4all, VR 808) from Srpski Miletić and 1 (VR 808) from Stapar; during 2016 in 2 (Crisps4all, Brooke) from Srpski Miletić, 2 (Lady Claire, VR 808) from Stapar, 2 (Lady Claire, Pirol) from Sombor and 1 (Panda) from Boleč. Milky-white, with pink to red centre, flat, irregular, fluidal colonies formed after 3-4 days of incubation on SMSA medium. A 553 bp and 288 bp fragments of 65 representative isolates were amplified using Ps-1/Ps-2 and OLY1/Y2 primer pairs, respectively. IF test proved presence of fluorescent bacterial cells. All strains caused wilting on tomato seedlings four days after inoculation. CONCLUSIONS: The results of this study detected presence of R. solanacearum on 11 potato samples isolated from four locations during period from 2013-2016.", publisher = "Udruženje mikrobiologa Srbije", journal = "12TH CONGRESS OF SERBIAN MICROBIOLOGISTS with international participation MICROMED 2018 REGIO", title = "MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM", pages = "210-209", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1928" }
Marković, S., Popović, T., Stanković, S.,& Jelušić, A.. (2018). MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM. in 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS with international participation MICROMED 2018 REGIO Udruženje mikrobiologa Srbije., 209-210. https://hdl.handle.net/21.15107/rcub_rimsi_1928
Marković S, Popović T, Stanković S, Jelušić A. MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM. in 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS with international participation MICROMED 2018 REGIO. 2018;:209-210. https://hdl.handle.net/21.15107/rcub_rimsi_1928 .
Marković, Sanja, Popović, Tatjana, Stanković, Slaviša, Jelušić, Aleksandra, "MONITORING OF POTATO FIELDS TO PRESENCE OF RALSTONIA SOLANACEARUM" in 12TH CONGRESS OF SERBIAN MICROBIOLOGISTS with international participation MICROMED 2018 REGIO (2018):209-210, https://hdl.handle.net/21.15107/rcub_rimsi_1928 .