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MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES ORIGINATED FROM POTATO IN SERBIA

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2019
bitstream_4996.pdf (1.075Mb)
Autori
Marković, Sanja
Stanković, Slaviša
Jelušić, Aleksandra
Iličić, Renata
Popović, Tatjana
Konferencijski prilog (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentu
Apstrakt
Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species including a wide range of crop plants, ornamentals and weeds. This bacterium is causing bacterial wilt disease which leads to severe economic losses. On potato, disease is known as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm temperate regions, in recent years cold-adapted strains dramatically enhanced the threat of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant pathogens in Europe. During six year period (2013-2018), isolates from diseased potato tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic analysis was performed with concatenated sequences of all tested isolates, and comp...ared with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic analysis placed tested isolates in the same cluster with R. solanacearum strains belonging to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused by this plant pathogenic bacteria is appearing in different locations and on different potato cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a quarantine bacteria occurring only on potato in our country, but considering its wide host range, there is a possibility of it causing disease on other significant crops with devastating consequences. Therefore, its fast and accurate identification and mapping is of main interest for stopping the disease spread.

Ključne reči:
Ralstonia solanacearum / bacterial wilt / brown rot / multilocus sequence analysis (mlsa)
Izvor:
6th CONGRESS OF THE SERBIAN GENETIC SOCIETY, 2019, 164-
Izdavač:
  • Serbian Genetic Society, Belgrade, Serbia
Finansiranje / projekti:
  • Ministry of Education, Science and Technological Development, Republic of Serbia (Project No. III43010)

ISBN: 978-86-87109-15-5

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rimsi_1923
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1923
Kolekcije
  • Radovi istraživača / Researchers’ publications
Institucija/grupa
Institut za multidisciplinarna istraživanja
TY  - CONF
AU  - Marković, Sanja
AU  - Stanković, Slaviša
AU  - Jelušić, Aleksandra
AU  - Iličić, Renata
AU  - Popović, Tatjana
PY  - 2019
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1923
AB  - Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species 
including a wide range of crop plants, ornamentals and weeds. This bacterium is causing 
bacterial wilt disease which leads to severe economic losses. On potato, disease is known 
as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm 
temperate regions, in recent years cold-adapted strains dramatically enhanced the threat 
of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant 
pathogens in Europe. During six year period (2013-2018), isolates from diseased potato 
tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked 
for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven 
housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic 
analysis was performed with concatenated sequences of all tested isolates, and compared 
with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates 
were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic 
analysis placed tested isolates in the same cluster with R. solanacearum strains belonging 
to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused 
by this plant pathogenic bacteria is appearing in different locations and on different potato 
cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a 
quarantine bacteria occurring only on potato in our country, but considering its wide host 
range, there is a possibility of it causing disease on other significant crops with devastating 
consequences. Therefore, its fast and accurate identification and mapping is of main interest 
for stopping the disease spread.
PB  - Serbian Genetic Society,  Belgrade, Serbia
C3  - 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
T1  - MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA
SP  - 164
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1923
ER  - 
@conference{
author = "Marković, Sanja and Stanković, Slaviša and Jelušić, Aleksandra and Iličić, Renata and Popović, Tatjana",
year = "2019",
abstract = "Ralstonia solanacearum is a soil borne bacterium which affects more than 450 plant species 
including a wide range of crop plants, ornamentals and weeds. This bacterium is causing 
bacterial wilt disease which leads to severe economic losses. On potato, disease is known 
as brown rot. Even though this phytopathogen originates from tropic, subtropics and warm 
temperate regions, in recent years cold-adapted strains dramatically enhanced the threat 
of European potato crops, including Serbia. R. solanacearum is at A2 list of quarantine plant 
pathogens in Europe. During six year period (2013-2018), isolates from diseased potato 
tubers were collected, identified using Multilocus sequence analysis (MLSA) and checked 
for certain phylotype affiliation. DNA from the obtained isolates was amplified using seven 
housekeeping genes (adk, fliC, gapA, gdhA, gyrB, hrpB, ppsA) and sequenced. Phylogenetic 
analysis was performed with concatenated sequences of all tested isolates, and compared 
with the most similar R. solanacearum strains from PAMDB database. All Serbian isolates 
were identified as R. solanacearum using PAMDB BLAST. Neighbor-joining phylogenetic 
analysis placed tested isolates in the same cluster with R. solanacearum strains belonging 
to race 3, biovar 2, and phylotype II obtained from PAMDB. Although, bacterial wilt caused 
by this plant pathogenic bacteria is appearing in different locations and on different potato 
cultivars in Serbia it remained genetically homogenous. Currently, R. solanacearum is a 
quarantine bacteria occurring only on potato in our country, but considering its wide host 
range, there is a possibility of it causing disease on other significant crops with devastating 
consequences. Therefore, its fast and accurate identification and mapping is of main interest 
for stopping the disease spread.",
publisher = "Serbian Genetic Society,  Belgrade, Serbia",
journal = "6th CONGRESS OF THE SERBIAN GENETIC SOCIETY",
title = "MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA",
pages = "164",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1923"
}
Marković, S., Stanković, S., Jelušić, A., Iličić, R.,& Popović, T.. (2019). MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY
Serbian Genetic Society,  Belgrade, Serbia., 164.
https://hdl.handle.net/21.15107/rcub_rimsi_1923
Marković S, Stanković S, Jelušić A, Iličić R, Popović T. MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA. in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY. 2019;:164.
https://hdl.handle.net/21.15107/rcub_rimsi_1923 .
Marković, Sanja, Stanković, Slaviša, Jelušić, Aleksandra, Iličić, Renata, Popović, Tatjana, "MULTILOCUS SEQUENCE ANALYSIS OF RALSTONIA SOLANACEARUM ISOLATES  ORIGINATED FROM POTATO IN SERBIA" in 6th CONGRESS OF THE SERBIAN GENETIC SOCIETY (2019):164,
https://hdl.handle.net/21.15107/rcub_rimsi_1923 .

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