Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters
Authors
Kolarević, StoimirMicsinai, Adrienn
Szántó-Egész, Réka
Lukács, Alena
Kračun-Kolarević, Margareta
Djordjevic, Ana
Jovanović Marić, Jovana
Vojnović Milutinović, Danijela
Kirschner, Alexander K. T.
Farnleitner, Andreas
Linke, Rita
Djukic, Aleksandar
Kostić-Vuković, Jovana

Sunjog, Karolina
Paunović, Momir
Conference object (Published version)

Metadata
Show full item recordAbstract
Background
Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater.
Objectives
The major objective of this study was to provide a more detailed insight in the potential of SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes.
Methodology
Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-...19 wave in Serbia that started in late February 2021.
SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel.
Results
Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.
Keywords:
Salmonella typhimurium / SOS/umuC / MTS test / ZFL cells / Danube riverSource:
FEMS Conference on Microbiology in association with Serbian Society of Microbiology, 2022, 614-Publisher:
- Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200007 (University of Belgrade, Institute for Biological Research 'Siniša Stanković') (RS-200007)
- Bilateral project of Republic of Serbia and Republic of Austria 2019-2021 (WTZ-SRB12-2018)
- Austrian Science Fund project P32464
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Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Kolarević, Stoimir AU - Micsinai, Adrienn AU - Szántó-Egész, Réka AU - Lukács, Alena AU - Kračun-Kolarević, Margareta AU - Djordjevic, Ana AU - Jovanović Marić, Jovana AU - Vojnović Milutinović, Danijela AU - Kirschner, Alexander K. T. AU - Farnleitner, Andreas AU - Linke, Rita AU - Djukic, Aleksandar AU - Kostić-Vuković, Jovana AU - Sunjog, Karolina AU - Paunović, Momir PY - 2022 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1920 AB - Background Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater. Objectives The major objective of this study was to provide a more detailed insight in the potential of SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes. Methodology Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021. SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel. Results Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment. PB - Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM) C3 - FEMS Conference on Microbiology in association with Serbian Society of Microbiology T1 - Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters SP - 614 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1920 ER -
@conference{ author = "Kolarević, Stoimir and Micsinai, Adrienn and Szántó-Egész, Réka and Lukács, Alena and Kračun-Kolarević, Margareta and Djordjevic, Ana and Jovanović Marić, Jovana and Vojnović Milutinović, Danijela and Kirschner, Alexander K. T. and Farnleitner, Andreas and Linke, Rita and Djukic, Aleksandar and Kostić-Vuković, Jovana and Sunjog, Karolina and Paunović, Momir", year = "2022", abstract = "Background Wastewater-based epidemiology surveillance of COVID-19 and other outbreaks in the future is a challenge for developing countries, as the majority of households are not connected to sewerage systems. In December 2019, we have detected SARS-CoV-2 RNA in the Danube River at a site that is severely affected by wastewater of Belgrade. Considering that rivers are much more complex systems in comparison to wastewater, additional efforts are needed to address all the factors which might influence the adoption of WBE as an alternative of targeting raw wastewater. Objectives The major objective of this study was to provide a more detailed insight in the potential of SARS-CoV-2 surveillance in Serbian surface waters under consideration of epidemiological, microbiological, physico-chemical and hydro-morphological parameters for epidemiological purposes. Methodology Water samples were collected at 12 sites at Sava and Danube Rivers in the Belgrade city area during the fourth COVID-19 wave in Serbia that started in late February 2021. SARS-CoV-2 RNA was quantified using RT-qPCR with primer sets targeting nucleocapsid (N1 and N2) and envelope (E) protein genes. Microbiological (standard fecal indicator bacteria and microbial faecal source tracking markers), epidemiological, physico-chemical and hydro-morphological parameters were analysed in parallel. Results Out of 44 samples analyzed, 31 were positive for at least one of the target regions of SARS-CoV-2. The results indicated that surveillance of SARS-CoV-2 RNA in surface waters in context with the large amount of epidemiological and environmental metadata can be used as epidemiological early-warning tool in countries with poor wastewater treatment.", publisher = "Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)", journal = "FEMS Conference on Microbiology in association with Serbian Society of Microbiology", title = "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters", pages = "614", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1920" }
Kolarević, S., Micsinai, A., Szántó-Egész, R., Lukács, A., Kračun-Kolarević, M., Djordjevic, A., Jovanović Marić, J., Vojnović Milutinović, D., Kirschner, A. K. T., Farnleitner, A., Linke, R., Djukic, A., Kostić-Vuković, J., Sunjog, K.,& Paunović, M.. (2022). Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology Federation of European Microbiological Societies in association with Serbian Society for Microbiology (SSM)., 614. https://hdl.handle.net/21.15107/rcub_rimsi_1920
Kolarević S, Micsinai A, Szántó-Egész R, Lukács A, Kračun-Kolarević M, Djordjevic A, Jovanović Marić J, Vojnović Milutinović D, Kirschner AKT, Farnleitner A, Linke R, Djukic A, Kostić-Vuković J, Sunjog K, Paunović M. Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters. in FEMS Conference on Microbiology in association with Serbian Society of Microbiology. 2022;:614. https://hdl.handle.net/21.15107/rcub_rimsi_1920 .
Kolarević, Stoimir, Micsinai, Adrienn, Szántó-Egész, Réka, Lukács, Alena, Kračun-Kolarević, Margareta, Djordjevic, Ana, Jovanović Marić, Jovana, Vojnović Milutinović, Danijela, Kirschner, Alexander K. T., Farnleitner, Andreas, Linke, Rita, Djukic, Aleksandar, Kostić-Vuković, Jovana, Sunjog, Karolina, Paunović, Momir, "Wastewater based epidemiology in countries with poor wastewater treatment - SARS-CoV-2 RNA in surface waters" in FEMS Conference on Microbiology in association with Serbian Society of Microbiology (2022):614, https://hdl.handle.net/21.15107/rcub_rimsi_1920 .