Allelic profi le of Serbian Xanthomonas campestris pv. campestris isolates from cabbage
Abstract
Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of
cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect
proper cabbage growth, leading to head weight and quality losses and thereby drastically
reducing its marketing value. The pathogen is genetically diverse, which is evident from the
presence of eleven races worldwide and more than thirty combinations of allelic profi les.
Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains
obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst
amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used
to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc
strains obtained from diff erent hosts and regions, whose allelic profi le...s had been determined
previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le
determination and Phyloviz software for constructing a minimum spanning tree. The obtained
allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and
rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides
with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No
connection between sequence type (ST) and the host was detected.
Keywords:
Xanthomonas campestris / black rot / cabbage / genetic diversity / allelic profileSource:
Pesticidi i fitomedicina, 2020, 35, 1, 19-26Publisher:
- Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200010 (Institute for Plant Protection and Environment, Belgrade) (RS-200010)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200053 (University of Belgrade, Institute for Multidisciplinary Research) (RS-200053)
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - JOUR AU - Popović, Tatjana AU - Jelušić, Aleksandra AU - Mitrović, Petar AU - Iličić, Renata AU - Marković, Sanja PY - 2020 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1916 AB - Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profi les. Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc strains obtained from diff erent hosts and regions, whose allelic profi les had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected. PB - Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade T2 - Pesticidi i fitomedicina T1 - Allelic profi le of Serbian Xanthomonas campestris pv. campestris isolates from cabbage EP - 26 IS - 1 SP - 19 VL - 35 DO - 10.2298/PIF2001019P ER -
@article{ author = "Popović, Tatjana and Jelušić, Aleksandra and Mitrović, Petar and Iličić, Renata and Marković, Sanja", year = "2020", abstract = "Xanthomonas campestris pv. campestris (Xcc), the causal agent of black rot disease of cabbage (Brassica oleracea var. capitata L.), is one of the most important bacteria which aff ect proper cabbage growth, leading to head weight and quality losses and thereby drastically reducing its marketing value. The pathogen is genetically diverse, which is evident from the presence of eleven races worldwide and more than thirty combinations of allelic profi les. Therefore, this study aimed to determine the allelic profi les of Serbian cabbage Xcc strains obtained in 2014. The analysis was done on three selected Xcc strains whose DNA was fi rst amplifi ed using polymerase chain reaction (PCR) with four housekeeping genes – P-X dnaK, fyuA, gyrB, and rpoD, then sequenced, and the obtained sequences were fi nally used to determine allelic profi les. Allelic profi les were determined by comparison with 33 Xcc strains obtained from diff erent hosts and regions, whose allelic profi les had been determined previously. A non-redundant database (NRDB) from the pubMLST was used for allelic profi le determination and Phyloviz software for constructing a minimum spanning tree. The obtained allelic profi le of all Serbian Xcc cabbage strains was 1, 3, 1, 1 for the P-X-dnaK, fyuA, gyrB and rpoD genes, respectively. This profi le is assigned as sequence type 2 (ST2) and it coincides with a Portuguese B. oleracea Xcc strain, CPBF 213, originating from B. oleracea var. costata. No connection between sequence type (ST) and the host was detected.", publisher = "Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade", journal = "Pesticidi i fitomedicina", title = "Allelic profi le of Serbian Xanthomonas campestris pv. campestris isolates from cabbage", pages = "26-19", number = "1", volume = "35", doi = "10.2298/PIF2001019P" }
Popović, T., Jelušić, A., Mitrović, P., Iličić, R.,& Marković, S.. (2020). Allelic profi le of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina Institute of Pesticides and Environmental Protection, Belgrade & Plant Protection Society of Serbia, Belgrade., 35(1), 19-26. https://doi.org/10.2298/PIF2001019P
Popović T, Jelušić A, Mitrović P, Iličić R, Marković S. Allelic profi le of Serbian Xanthomonas campestris pv. campestris isolates from cabbage. in Pesticidi i fitomedicina. 2020;35(1):19-26. doi:10.2298/PIF2001019P .
Popović, Tatjana, Jelušić, Aleksandra, Mitrović, Petar, Iličić, Renata, Marković, Sanja, "Allelic profi le of Serbian Xanthomonas campestris pv. campestris isolates from cabbage" in Pesticidi i fitomedicina, 35, no. 1 (2020):19-26, https://doi.org/10.2298/PIF2001019P . .