Rhinusa pilosa (Gyllenhaal) (Coleoptera, Curculionidae) induces galls in Linaria vulgaris Mill., Yellow or
common toadflax (Plantaginaceae). Gall is defined as atypical plant growth induced by host-specific organisms,
and represents larval chamber which provides food and protection during insect development. It has
been shown that gall formation is triggered by ovoipositional fluid (cecidogen). In order to determine which
cecidogen compounds may have bioactive properties, we performed comparative analysis of phenolics profiles
of cecidogen and stem extracts of L. vulgaris using UHPLC coupled with OrbiTrap mass analyzer. In addition,
we analyzed the effects of feeding, oviposition and early gall development on Class III peroxidase activity
(POD, EC 1.11.1.7) and phenolic content in the stems of L. vulgaris during seven days. Out of 55 phenolic
compounds identified in cecidogen and L. vulgaris stem, one unknown phenolic glycoside was found only in
cecidogen, which produced an MS...2 base peak at 387 m/z, and 327 m/z and 267 m/z base peaks at MS3 and
MS4 fragmentation, respectively. Gall development during seven days was accompanied by decreased concentrations
of hydroxybenzoic, hydroxycinnamic acids and flavonoids and by slight lignin deposition. An oscillatory
induction of POD activity, with the first peak obtained 3 h after oviposition, was related to oxidative
burst during stem wounding and oviposition. Overall results suggested the importance of phenolics in stem
structural changes and regulation of plant metabolism induced by R. pilosa.
2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015, 2015, 110-
Издавач:
Serbian Plant Physiology Society
Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
TY - CONF
AU - Sedlarević Zorić, Ana
AU - Morina, Filis
AU - Tosevski, Ivo
AU - Gašić, Uroš
AU - Jovic, Jelena
AU - Krstic, Oliver
AU - Veljović-Jovanović, Sonja
PY - 2015
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1877
AB - Rhinusa pilosa (Gyllenhaal) (Coleoptera, Curculionidae) induces galls in Linaria vulgaris Mill., Yellow or
common toadflax (Plantaginaceae). Gall is defined as atypical plant growth induced by host-specific organisms,
and represents larval chamber which provides food and protection during insect development. It has
been shown that gall formation is triggered by ovoipositional fluid (cecidogen). In order to determine which
cecidogen compounds may have bioactive properties, we performed comparative analysis of phenolics profiles
of cecidogen and stem extracts of L. vulgaris using UHPLC coupled with OrbiTrap mass analyzer. In addition,
we analyzed the effects of feeding, oviposition and early gall development on Class III peroxidase activity
(POD, EC 1.11.1.7) and phenolic content in the stems of L. vulgaris during seven days. Out of 55 phenolic
compounds identified in cecidogen and L. vulgaris stem, one unknown phenolic glycoside was found only in
cecidogen, which produced an MS2 base peak at 387 m/z, and 327 m/z and 267 m/z base peaks at MS3 and
MS4 fragmentation, respectively. Gall development during seven days was accompanied by decreased concentrations
of hydroxybenzoic, hydroxycinnamic acids and flavonoids and by slight lignin deposition. An oscillatory
induction of POD activity, with the first peak obtained 3 h after oviposition, was related to oxidative
burst during stem wounding and oviposition. Overall results suggested the importance of phenolics in stem
structural changes and regulation of plant metabolism induced by R. pilosa.
PB - Serbian Plant Physiology Society
PB - Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade
C3 - 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015
T1 - Identification of phenolic compounds in cecidogen and Linaria vulgaris. Changes in phenolic metabolism during gall formation induced by Rhinusa pilosa
SP - 110
UR - https://hdl.handle.net/21.15107/rcub_rimsi_1877
ER -
@conference{
author = "Sedlarević Zorić, Ana and Morina, Filis and Tosevski, Ivo and Gašić, Uroš and Jovic, Jelena and Krstic, Oliver and Veljović-Jovanović, Sonja",
year = "2015",
abstract = "Rhinusa pilosa (Gyllenhaal) (Coleoptera, Curculionidae) induces galls in Linaria vulgaris Mill., Yellow or
common toadflax (Plantaginaceae). Gall is defined as atypical plant growth induced by host-specific organisms,
and represents larval chamber which provides food and protection during insect development. It has
been shown that gall formation is triggered by ovoipositional fluid (cecidogen). In order to determine which
cecidogen compounds may have bioactive properties, we performed comparative analysis of phenolics profiles
of cecidogen and stem extracts of L. vulgaris using UHPLC coupled with OrbiTrap mass analyzer. In addition,
we analyzed the effects of feeding, oviposition and early gall development on Class III peroxidase activity
(POD, EC 1.11.1.7) and phenolic content in the stems of L. vulgaris during seven days. Out of 55 phenolic
compounds identified in cecidogen and L. vulgaris stem, one unknown phenolic glycoside was found only in
cecidogen, which produced an MS2 base peak at 387 m/z, and 327 m/z and 267 m/z base peaks at MS3 and
MS4 fragmentation, respectively. Gall development during seven days was accompanied by decreased concentrations
of hydroxybenzoic, hydroxycinnamic acids and flavonoids and by slight lignin deposition. An oscillatory
induction of POD activity, with the first peak obtained 3 h after oviposition, was related to oxidative
burst during stem wounding and oviposition. Overall results suggested the importance of phenolics in stem
structural changes and regulation of plant metabolism induced by R. pilosa.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković” – National Institute of Republic of Serbia, University of Belgrade",
journal = "2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015",
title = "Identification of phenolic compounds in cecidogen and Linaria vulgaris. Changes in phenolic metabolism during gall formation induced by Rhinusa pilosa",
pages = "110",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1877"
}
Sedlarević Zorić, A., Morina, F., Tosevski, I., Gašić, U., Jovic, J., Krstic, O.,& Veljović-Jovanović, S.. (2015). Identification of phenolic compounds in cecidogen and Linaria vulgaris. Changes in phenolic metabolism during gall formation induced by Rhinusa pilosa. in 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015
Serbian Plant Physiology Society., 110.
https://hdl.handle.net/21.15107/rcub_rimsi_1877
Sedlarević Zorić A, Morina F, Tosevski I, Gašić U, Jovic J, Krstic O, Veljović-Jovanović S. Identification of phenolic compounds in cecidogen and Linaria vulgaris. Changes in phenolic metabolism during gall formation induced by Rhinusa pilosa. in 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015. 2015;:110.
https://hdl.handle.net/21.15107/rcub_rimsi_1877 .
Sedlarević Zorić, Ana, Morina, Filis, Tosevski, Ivo, Gašić, Uroš, Jovic, Jelena, Krstic, Oliver, Veljović-Jovanović, Sonja, "Identification of phenolic compounds in cecidogen and Linaria vulgaris. Changes in phenolic metabolism during gall formation induced by Rhinusa pilosa" in 2st International Conference on Plant Biology • 21th Symposium of the Serbian Plant Physiology Society • COST ACTION FA1106 QUALITYFRUIT Workshop Petnica Science Center 17-20 june, 2015 (2015):110,
https://hdl.handle.net/21.15107/rcub_rimsi_1877 .
