Optimization of conditions for glutaraldehyde immobilization of soyabean peroxidase
Authors
Prokopijević, Miloš
Prodanović, Olivera

Spasojević, Dragica

Stojanović, Željko
Radotić, Ksenija

Prodanović, Radivoje
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Phenols are considered priority pollutants of wastewaters, persistently present in the environment and its conventional treatment could be overcome using enzymatic methods. Although the application of enzymes, such as peroxidases, has been investigated widely, its main disadvantage is the cost of purified enzymes. Immobilized enzymes, on the other hand, have the advantage of greater stability and easy separation from the reaction medium, which makes them reusable. The aim of this study was to optimize the conditions for soyabean peroxidase glutaraldehyde immobilization technique.
Immobilization was done in small tubes, by mixing 1 ml of enzyme solution (0.05-2.5 mg/ml) with 50 mg of support matrix (macroporous glycidyl methacrylates previosly synthesized in our lab) on a rotary shaker
(24h at 25°C, 250 rpm). Enzyme activity was measured using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and the reaction products were measured following absorbance at 420 nm, for ...3 min
using a spectrophotometer.
Our data demonstrate that soyabean peroxidase can be successfully immobilized on macroporous glycidyl methacrylate using glutaraldehyde activation.
Keywords:
soybean peroxidase / glycidyl methacrylate / immobilization / pyrogallol / glutaraldehydeSource:
Naučni skup sa medjunarodnim učešćem Zaštita prirode u 21 vijeku, Žabljak, Crna Gora, 2011, 2, 925-Publisher:
- AP Print, Podgorica
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Institut za multidisciplinarna istraživanjaTY - CONF AU - Prokopijević, Miloš AU - Prodanović, Olivera AU - Spasojević, Dragica AU - Stojanović, Željko AU - Radotić, Ksenija AU - Prodanović, Radivoje PY - 2011 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1866 AB - Phenols are considered priority pollutants of wastewaters, persistently present in the environment and its conventional treatment could be overcome using enzymatic methods. Although the application of enzymes, such as peroxidases, has been investigated widely, its main disadvantage is the cost of purified enzymes. Immobilized enzymes, on the other hand, have the advantage of greater stability and easy separation from the reaction medium, which makes them reusable. The aim of this study was to optimize the conditions for soyabean peroxidase glutaraldehyde immobilization technique. Immobilization was done in small tubes, by mixing 1 ml of enzyme solution (0.05-2.5 mg/ml) with 50 mg of support matrix (macroporous glycidyl methacrylates previosly synthesized in our lab) on a rotary shaker (24h at 25°C, 250 rpm). Enzyme activity was measured using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and the reaction products were measured following absorbance at 420 nm, for 3 min using a spectrophotometer. Our data demonstrate that soyabean peroxidase can be successfully immobilized on macroporous glycidyl methacrylate using glutaraldehyde activation. PB - AP Print, Podgorica C3 - Naučni skup sa medjunarodnim učešćem Zaštita prirode u 21 vijeku, Žabljak, Crna Gora T1 - Optimization of conditions for glutaraldehyde immobilization of soyabean peroxidase SP - 925 VL - 2 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1866 ER -
@conference{ author = "Prokopijević, Miloš and Prodanović, Olivera and Spasojević, Dragica and Stojanović, Željko and Radotić, Ksenija and Prodanović, Radivoje", year = "2011", abstract = "Phenols are considered priority pollutants of wastewaters, persistently present in the environment and its conventional treatment could be overcome using enzymatic methods. Although the application of enzymes, such as peroxidases, has been investigated widely, its main disadvantage is the cost of purified enzymes. Immobilized enzymes, on the other hand, have the advantage of greater stability and easy separation from the reaction medium, which makes them reusable. The aim of this study was to optimize the conditions for soyabean peroxidase glutaraldehyde immobilization technique. Immobilization was done in small tubes, by mixing 1 ml of enzyme solution (0.05-2.5 mg/ml) with 50 mg of support matrix (macroporous glycidyl methacrylates previosly synthesized in our lab) on a rotary shaker (24h at 25°C, 250 rpm). Enzyme activity was measured using pyrogallol and H2O2 as substrates (13 mM and 10 mM, respectively), and the reaction products were measured following absorbance at 420 nm, for 3 min using a spectrophotometer. Our data demonstrate that soyabean peroxidase can be successfully immobilized on macroporous glycidyl methacrylate using glutaraldehyde activation.", publisher = "AP Print, Podgorica", journal = "Naučni skup sa medjunarodnim učešćem Zaštita prirode u 21 vijeku, Žabljak, Crna Gora", title = "Optimization of conditions for glutaraldehyde immobilization of soyabean peroxidase", pages = "925", volume = "2", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1866" }
Prokopijević, M., Prodanović, O., Spasojević, D., Stojanović, Ž., Radotić, K.,& Prodanović, R.. (2011). Optimization of conditions for glutaraldehyde immobilization of soyabean peroxidase. in Naučni skup sa medjunarodnim učešćem Zaštita prirode u 21 vijeku, Žabljak, Crna Gora AP Print, Podgorica., 2, 925. https://hdl.handle.net/21.15107/rcub_rimsi_1866
Prokopijević M, Prodanović O, Spasojević D, Stojanović Ž, Radotić K, Prodanović R. Optimization of conditions for glutaraldehyde immobilization of soyabean peroxidase. in Naučni skup sa medjunarodnim učešćem Zaštita prirode u 21 vijeku, Žabljak, Crna Gora. 2011;2:925. https://hdl.handle.net/21.15107/rcub_rimsi_1866 .
Prokopijević, Miloš, Prodanović, Olivera, Spasojević, Dragica, Stojanović, Željko, Radotić, Ksenija, Prodanović, Radivoje, "Optimization of conditions for glutaraldehyde immobilization of soyabean peroxidase" in Naučni skup sa medjunarodnim učešćem Zaštita prirode u 21 vijeku, Žabljak, Crna Gora, 2 (2011):925, https://hdl.handle.net/21.15107/rcub_rimsi_1866 .