Effect of manganese on antioxidant activity in maize

Abstract

Manganese (Mn) is an essential element for plant growth. For metabolic function, Mn2+ is required at low concentrations, but Mn2+ excess can change the activity of various enzymes and interfere with the absorption and translocation of some minerals such as Ca, Mg, Fe, and P. Mn availability to the plants depends on pH of the growth medium and on root exudates for Mn chelation or reduction. The main aim of this research was to investigate the effect of Mn2+ on the main parameters of secondary metabolism: total phenolic content (TPC) and total antioxidative activity (TAA) in maize (Zea mays L.) shoots and roots. Maize was chosen as agricultural species important for human and animal nutrition, and a simply cultivated plant. Maize seeds (inbred line Va35) were germinated in distilled water and then transferred into the hydroponic (KNOP/2) growth solution. Mn2+ was applied in two different concentrations (5 and 100 μM) by adding MnSO4 into the hydroponic solution. After 7 days (December 2021) of growing under 8/16h photoperiod, the leaves and roots were collected. The TPC was measured according to Folin-Ciocalteu’s spectrophotometric procedure, while TAA was determined by ABTS/HRP assay. The results showed that TAA was significantly decreased only in maize roots after the treatment with 5 μM Mn2+, while higher Mn2+ concentration (100 μM) did not change TAA in maize roots. TAA in shoots and TPC in both parts of maize was unchanged. Finding that 5 μM of Mn2+ have decreasing effect on maize antioxidative activity without harming plants indicates that lower concentration can be more toxic due to: 1. plants excreting an excess of Mn2+ into the environment after the treatment with high Mn2+ concentration and 2. Mn can affect the metabolism of small molecules (enzymes or phytochelatins) binding for them and decreasing the total ROS scavenging activity.