In this work, two non-invasive and independent methods for the
characterization of aflatoxin-stressed wheat seeds were applied.Aflatoxins
(AFs) are secondary toxic fungi metabolites, which exhibit hazardous effects
on plants (phytotoxic), as well as on humans and animals. First, for 2D EPR
imaging, we used 3-CP spin probe for the estimation of changes in the redox
state of the aflatoxin-stressed seed. On the other side, an excitation-emission
fluorescence approach was used to evaluate the spectral profiles of the key
biological molecules. We determined their areas and the ratio of the two
emission peaks at 355 nm (Ex 290) and 430 nm (Ex 350), corresponding to
protein and phenol emission, respectively. Our results showed that the ratio
of the two peaks (p/f) decreased in the presence of AFs. Both methods
revealed to be useful for the rapid, non-invasive assessment of the seeds'
stress state induced by AFs.
Keywords:
aflatoxin / FLUORESCENCE / EPR IMAGING
Source:
PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 2018, 523-526
Publisher:
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
TY - CONF
AU - Bartolić, Dragana
AU - Stanković, Mira
AU - Maksimović, Vuk
AU - Mutavdžić, Dragosav
AU - Radotić, Ksenija
PY - 2018
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1814
AB - In this work, two non-invasive and independent methods for the
characterization of aflatoxin-stressed wheat seeds were applied.Aflatoxins
(AFs) are secondary toxic fungi metabolites, which exhibit hazardous effects
on plants (phytotoxic), as well as on humans and animals. First, for 2D EPR
imaging, we used 3-CP spin probe for the estimation of changes in the redox
state of the aflatoxin-stressed seed. On the other side, an excitation-emission
fluorescence approach was used to evaluate the spectral profiles of the key
biological molecules. We determined their areas and the ratio of the two
emission peaks at 355 nm (Ex 290) and 430 nm (Ex 350), corresponding to
protein and phenol emission, respectively. Our results showed that the ratio
of the two peaks (p/f) decreased in the presence of AFs. Both methods
revealed to be useful for the rapid, non-invasive assessment of the seeds'
stress state induced by AFs.
PB - Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia
C3 - PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
T1 - NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY
EP - 526
SP - 523
UR - https://hdl.handle.net/21.15107/rcub_rimsi_1814
ER -
@conference{
author = "Bartolić, Dragana and Stanković, Mira and Maksimović, Vuk and Mutavdžić, Dragosav and Radotić, Ksenija",
year = "2018",
abstract = "In this work, two non-invasive and independent methods for the
characterization of aflatoxin-stressed wheat seeds were applied.Aflatoxins
(AFs) are secondary toxic fungi metabolites, which exhibit hazardous effects
on plants (phytotoxic), as well as on humans and animals. First, for 2D EPR
imaging, we used 3-CP spin probe for the estimation of changes in the redox
state of the aflatoxin-stressed seed. On the other side, an excitation-emission
fluorescence approach was used to evaluate the spectral profiles of the key
biological molecules. We determined their areas and the ratio of the two
emission peaks at 355 nm (Ex 290) and 430 nm (Ex 350), corresponding to
protein and phenol emission, respectively. Our results showed that the ratio
of the two peaks (p/f) decreased in the presence of AFs. Both methods
revealed to be useful for the rapid, non-invasive assessment of the seeds'
stress state induced by AFs.",
publisher = "Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia",
journal = "PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry",
title = "NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY",
pages = "526-523",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1814"
}
Bartolić, D., Stanković, M., Maksimović, V., Mutavdžić, D.,& Radotić, K.. (2018). NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY. in PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry
Society of Physical Chemists of Serbia, Studentski Trg 12-16, 11158, Belgrade, Serbia., 523-526.
https://hdl.handle.net/21.15107/rcub_rimsi_1814
Bartolić D, Stanković M, Maksimović V, Mutavdžić D, Radotić K. NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY. in PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2018;:523-526.
https://hdl.handle.net/21.15107/rcub_rimsi_1814 .
Bartolić, Dragana, Stanković, Mira, Maksimović, Vuk, Mutavdžić, Dragosav, Radotić, Ksenija, "NON-INVASIVE CHARACTERIZATIONOF AFLATOXIN-STRESSED WHEAT SEEDSUSING 2D EPR IMAGING AND EEM FLUORESCENCE SPECTROSCOPY" in PHYSICAL CHEMISTRY 2018 - 14th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2018):523-526,
https://hdl.handle.net/21.15107/rcub_rimsi_1814 .
