INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS
Апстракт
UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores.
Кључне речи:
soybean / UV-B / fluorescence spectroscopyИзвор:
15th International Conference on Fundamental and Applied Aspects of Physical Chemistry, 2021, 336-339Издавач:
- Society of Physical Chemists of Serbia
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200053 (Универзитет у Београду, Институт за мултидисциплинарна истраживања) (RS-MESTD-inst-2020-200053)
Институција/група
Institut za multidisciplinarna istraživanjaTY - CONF AU - Bartolić, Dragana AU - Stanković, Mira AU - Prokopijević, Miloš AU - Radotić, Ksenija PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1795 AB - UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores. PB - Society of Physical Chemists of Serbia C3 - 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry T1 - INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS EP - 339 SP - 336 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1795 ER -
@conference{ author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Radotić, Ksenija", year = "2021", abstract = "UV-B light, plays a crucial role as a signal for inducing plant response and development of specific photomorphogenic responses. The UV radiation may have a damaging effect on cellular components and macromolecules in seeds, which may influence seed quality. We compared Glycine max L. seeds exposed to UV-B radiation for 1 h with non-irradiated seeds (control). The antioxidant activity was estimated using a DPPH assay. The seeds’ fluorescence characteristics were studied by fluorescence excitation-emission matrices. According to the obtained results the UV-B irradiated seeds possess a significantly higher free radical scavenging activity compared to the control. The fluorescence analysis showed differences in the spectral emission profiles of irradiated seeds compared to the control seeds. The results imply that 1h seed exposure to UV-B increases free radical content which may alter the structures of cellular macromolecules resulting in degradation of some of the fluorophores.", publisher = "Society of Physical Chemists of Serbia", journal = "15th International Conference on Fundamental and Applied Aspects of Physical Chemistry", title = "INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS", pages = "339-336", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1795" }
Bartolić, D., Stanković, M., Prokopijević, M.,& Radotić, K.. (2021). INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry Society of Physical Chemists of Serbia., 336-339. https://hdl.handle.net/21.15107/rcub_rimsi_1795
Bartolić D, Stanković M, Prokopijević M, Radotić K. INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS. in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry. 2021;:336-339. https://hdl.handle.net/21.15107/rcub_rimsi_1795 .
Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Radotić, Ksenija, "INFLUENCE OF ULTRAVIOLET B (UV-B) IRRADIATION ON ANTIOXIDANT CAPACITY AND FLUORESCENCE CHARACTERISTICS OF SOYBEAN (GLYCINE MAX L.) SEEDS" in 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry (2021):336-339, https://hdl.handle.net/21.15107/rcub_rimsi_1795 .