Antioxidant activity estimation of inner and outer seed fractions of the legumes Vigna radiata L. and Glycine max L.
Abstract
Legumes have multiple functions in sustainable agriculture, but also are a favourable ingredient
of functional nutrition. Antioxidants in legumes have several beneficial physiological properties
and provide protection against chronic diseases. In this study, we compared the antioxidant
activities of the seed fractions (outer and inner) for two different legumes: mung bean (Vigna
radiata L.) and soybean (Glycine max L). The antioxidant activity was estimated using a
modified DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay in a 96-well microplate. We showed that
mung bean hulls possessed significantly higher (p<0.05) DPPH free radical scavenging activity
(80.80 % ± 0.19) compared to their inner fraction (10.94 % ± 0.23), as well as to both fractions
of the soybean. On the other hand, the soybean seeds' inner fractions (21.00 % ± 0.32) exhibited
a significantly (p<0.05) higher activity than the hulls (8.78 % ± 0.71) and the inner fraction of
the mung bean. The obtained results indic...ated that in each of the two analysed legume species,
inner and outer seed fractions exhibited different antioxidant activities regarding to the
elimination of the free radicals. The obtained results indicate that antioxidant capacity may be a
useful indicator in the estimation of the quality of legume seeds as food and feed.
Keywords:
Antioxidant activity / Soybean / Mung bean / DPPH / Food qualitySource:
Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, Jahorina, October 07-10, 2021, Bosnia and Herzegovina, 2021, 154-158Publisher:
- University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia
Funding / projects:
Collections
Institution/Community
Institut za multidisciplinarna istraživanjaTY - CONF AU - Bartolić, Dragana AU - Prokopijević, Miloš AU - Radotić, Ksenija PY - 2021 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1784 AB - Legumes have multiple functions in sustainable agriculture, but also are a favourable ingredient of functional nutrition. Antioxidants in legumes have several beneficial physiological properties and provide protection against chronic diseases. In this study, we compared the antioxidant activities of the seed fractions (outer and inner) for two different legumes: mung bean (Vigna radiata L.) and soybean (Glycine max L). The antioxidant activity was estimated using a modified DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay in a 96-well microplate. We showed that mung bean hulls possessed significantly higher (p<0.05) DPPH free radical scavenging activity (80.80 % ± 0.19) compared to their inner fraction (10.94 % ± 0.23), as well as to both fractions of the soybean. On the other hand, the soybean seeds' inner fractions (21.00 % ± 0.32) exhibited a significantly (p<0.05) higher activity than the hulls (8.78 % ± 0.71) and the inner fraction of the mung bean. The obtained results indicated that in each of the two analysed legume species, inner and outer seed fractions exhibited different antioxidant activities regarding to the elimination of the free radicals. The obtained results indicate that antioxidant capacity may be a useful indicator in the estimation of the quality of legume seeds as food and feed. PB - University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia C3 - Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, Jahorina, October 07-10, 2021, Bosnia and Herzegovina T1 - Antioxidant activity estimation of inner and outer seed fractions of the legumes Vigna radiata L. and Glycine max L. EP - 158 SP - 154 UR - https://hdl.handle.net/21.15107/rcub_rimsi_1784 ER -
@conference{ author = "Bartolić, Dragana and Prokopijević, Miloš and Radotić, Ksenija", year = "2021", abstract = "Legumes have multiple functions in sustainable agriculture, but also are a favourable ingredient of functional nutrition. Antioxidants in legumes have several beneficial physiological properties and provide protection against chronic diseases. In this study, we compared the antioxidant activities of the seed fractions (outer and inner) for two different legumes: mung bean (Vigna radiata L.) and soybean (Glycine max L). The antioxidant activity was estimated using a modified DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay in a 96-well microplate. We showed that mung bean hulls possessed significantly higher (p<0.05) DPPH free radical scavenging activity (80.80 % ± 0.19) compared to their inner fraction (10.94 % ± 0.23), as well as to both fractions of the soybean. On the other hand, the soybean seeds' inner fractions (21.00 % ± 0.32) exhibited a significantly (p<0.05) higher activity than the hulls (8.78 % ± 0.71) and the inner fraction of the mung bean. The obtained results indicated that in each of the two analysed legume species, inner and outer seed fractions exhibited different antioxidant activities regarding to the elimination of the free radicals. The obtained results indicate that antioxidant capacity may be a useful indicator in the estimation of the quality of legume seeds as food and feed.", publisher = "University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia", journal = "Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, Jahorina, October 07-10, 2021, Bosnia and Herzegovina", title = "Antioxidant activity estimation of inner and outer seed fractions of the legumes Vigna radiata L. and Glycine max L.", pages = "158-154", url = "https://hdl.handle.net/21.15107/rcub_rimsi_1784" }
Bartolić, D., Prokopijević, M.,& Radotić, K.. (2021). Antioxidant activity estimation of inner and outer seed fractions of the legumes Vigna radiata L. and Glycine max L.. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, Jahorina, October 07-10, 2021, Bosnia and Herzegovina University of East Sarajevo, Faculty of Agriculture, Republic of Srpska, Bosnia., 154-158. https://hdl.handle.net/21.15107/rcub_rimsi_1784
Bartolić D, Prokopijević M, Radotić K. Antioxidant activity estimation of inner and outer seed fractions of the legumes Vigna radiata L. and Glycine max L.. in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, Jahorina, October 07-10, 2021, Bosnia and Herzegovina. 2021;:154-158. https://hdl.handle.net/21.15107/rcub_rimsi_1784 .
Bartolić, Dragana, Prokopijević, Miloš, Radotić, Ksenija, "Antioxidant activity estimation of inner and outer seed fractions of the legumes Vigna radiata L. and Glycine max L." in Proceedings of the XII International Scientific Agricultural Symposium “Agrosym 2021”, Jahorina, October 07-10, 2021, Bosnia and Herzegovina (2021):154-158, https://hdl.handle.net/21.15107/rcub_rimsi_1784 .