Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period o...f exposure, induce biochemical and biophysical changes in the soybean seeds.
TY - JOUR
AU - Bartolić, Dragana
AU - Stanković, Mira
AU - Prokopijević, Miloš
AU - Radotić, Ksenija
PY - 2022
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1772
AB - Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period of exposure, induce biochemical and biophysical changes in the soybean seeds.
PB - Pleiades Publishing, Ltd.
T2 - Russian Journal of Physical Chemistry A
T1 - Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds
EP - 2800
IS - 12
SP - 2797
VL - 96
DO - 10.1134/S0036024422120044
ER -
@article{
author = "Bartolić, Dragana and Stanković, Mira and Prokopijević, Miloš and Radotić, Ksenija",
year = "2022",
abstract = "Plant secondary metabolism, as well as the growth and development of specific photomorphogenic
responses, can be induced by UV light. The effects of UV-A and UV-B irradiations on the antioxidant activity and fluorescence characteristics of Glycine max L. seeds were investigated for two different exposure periods (1, 3 h). The antioxidant activity and photoluminescent characteristics of irradiated seeds were evaluated by the DPPH (2,2'-diphenyl-1-picrylhydrazyl radical) assay and excitation-emission matrices (EEMs) fluorescence spectroscopy, respectively. The DPPH scavenging activity significantly increased with exposure time in the UV-A treated seeds, while variations were observed after UV-B treatment. EEM showed three main peaks characterized with excitation/emission 280/355 nm (Peak A), 290/400 nm (Peak B), and 370/465 nm (Peak C). The most prominent change in fluorescence intensity was observed in peak B. Obtained results indicate that the type of UV radiation, as well as period of exposure, induce biochemical and biophysical changes in the soybean seeds.",
publisher = "Pleiades Publishing, Ltd.",
journal = "Russian Journal of Physical Chemistry A",
title = "Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds",
pages = "2800-2797",
number = "12",
volume = "96",
doi = "10.1134/S0036024422120044"
}
Bartolić, D., Stanković, M., Prokopijević, M.,& Radotić, K.. (2022). Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds. in Russian Journal of Physical Chemistry A
Pleiades Publishing, Ltd.., 96(12), 2797-2800.
https://doi.org/10.1134/S0036024422120044
Bartolić D, Stanković M, Prokopijević M, Radotić K. Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds. in Russian Journal of Physical Chemistry A. 2022;96(12):2797-2800.
doi:10.1134/S0036024422120044 .
Bartolić, Dragana, Stanković, Mira, Prokopijević, Miloš, Radotić, Ksenija, "Effects of UV-A and UV-B Irradiation on Antioxidant Activity and Fluorescence Characteristics of Soybean (Glycine max L.) Seeds" in Russian Journal of Physical Chemistry A, 96, no. 12 (2022):2797-2800,
https://doi.org/10.1134/S0036024422120044 . .
