Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.
Keywords:
alginate / horseradish peroxidase / chemical modification / immobilization
Source:
29th International Conference Ecological Truth and Environmental Research - EcoTER'22, 2022, 360-363
TY - CONF
AU - Spasojević, Dragica
AU - Prokopijević, Miloš
AU - Prodanović, Olivera
AU - Pantić, Nevena
AU - Stanković, Mira
AU - Radotić, Ksenija
AU - Prodanović, Radivoje
PY - 2022
UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1767
AB - Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.
PB - University of Belgrade, Technical Faculty in Bor
C3 - 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
T1 - PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP
EP - 363
SP - 360
UR - https://hdl.handle.net/21.15107/rcub_rimsi_1767
ER -
@conference{
author = "Spasojević, Dragica and Prokopijević, Miloš and Prodanović, Olivera and Pantić, Nevena and Stanković, Mira and Radotić, Ksenija and Prodanović, Radivoje",
year = "2022",
abstract = "Alginate is a natural polymer present in the cell wall of brown algae. Due to its many advantages, it
has been used extensively in the food industry, pharmacy, and biomedicine. To enhance properties,
such as stability and biodegradability, alginate is often chemically crosslinked. In this study, alginate
was crosslinked using N-hydroxysuccinimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
hydrochloride and tyramine hydrochloride. Horseradish peroxidase was self-immobilized within
hydrogel microbeads during the polymerization reaction. The glucose oxidase/glucose system
generates H2O2 internally, which can prevent the detrimental effect of excess peroxide. A small
amount of leaking enzyme shows potential for longer storage and reuse.",
publisher = "University of Belgrade, Technical Faculty in Bor",
journal = "29th International Conference Ecological Truth and Environmental Research - EcoTER'22",
title = "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP",
pages = "363-360",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1767"
}
Spasojević, D., Prokopijević, M., Prodanović, O., Pantić, N., Stanković, M., Radotić, K.,& Prodanović, R.. (2022). PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22
University of Belgrade, Technical Faculty in Bor., 360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767
Spasojević D, Prokopijević M, Prodanović O, Pantić N, Stanković M, Radotić K, Prodanović R. PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP. in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22. 2022;:360-363.
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .
Spasojević, Dragica, Prokopijević, Miloš, Prodanović, Olivera, Pantić, Nevena, Stanković, Mira, Radotić, Ksenija, Prodanović, Radivoje, "PREPARATION OF CROSSLINKED TYRAMINE-ALGINATE HYDROGEL USING EDC/NHS WITH SELF-IMMOBILIZED HRP" in 29th International Conference Ecological Truth and Environmental Research - EcoTER'22 (2022):360-363,
https://hdl.handle.net/21.15107/rcub_rimsi_1767 .
