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Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads

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2018
bitstream_4473.pdf (1.491Mb)
Authors
Pantić, Nevena
Popović, Nikolina
Prokopijević, Miloš
Spasojević, Dragica
Prodanović, Radivoje
Radotić, Ksenija
Prodanović, Olivera
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Abstract
Removal of phenolic compounds from wastewaters was previously studied using different enzymatic approaches. In the presence of hydrogen peroxide, peroxidases are able to oxidize phenol-like compounds and form non-soluble polymers that could be easily removed from aqueous phase. Horseradish peroxidase (HRP) is the most investigated peroxidase used for phenol removal from waste effluents, but it can be easily inactivated during this process by excess of hydrogen peroxide. In order to increase operational stability of the enzyme, immobilization on different materials and various peroxide delivery systems were tested. In our previous work, we studied bioinspired hydrogels based on natural cell wall polymers and enzymes, for efficient removal of phenols from water. In this work, tyramine-alginate hydrogels that we have previously developed were used for horseradish peroxidase encapsulation within micro-beads obtained in a coupled emulsion polymerization reaction. The aim of this research w...as to study the influence of tyramine-alginate concentration and hydrogen peroxide delivery system on operational stability and efficiency of phenol removal by immobilized peroxidase. The best result of 96% phenol removal from water solution was achieved by peroxidase immobilized within 20% (w/v) tyramine-alginate micro-beads using delivery system for hydrogen peroxide composed of 0.187 U mL-1 of glucose oxidase and 4 mmol L-1 of glucose. The reusability studies showed that these biocatalysts can be used up to five cycles with slight decrease in their catalytic performance.

Keywords:
immobilization / horseradish peroxidase / phenol removal / tyramine / alginate
Source:
3rd International Conference on Plant Biology (22nd SPPS Meeting), 2018, 157-
Publisher:
  • Serbian Plant Physiology Society
  • Institute for Biological Research “Siniša Stanković”, University of Belgrade
  • Faculty of Biology, University of Belgrade
Funding / projects:
  • Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)

ISBN: 978-86-912591-4-3

[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_rimsi_1764
URI
http://rimsi.imsi.bg.ac.rs/handle/123456789/1764
Collections
  • Radovi istraživača / Researchers’ publications
Institution/Community
Institut za multidisciplinarna istraživanja
TY  - CONF
AU  - Pantić, Nevena
AU  - Popović, Nikolina
AU  - Prokopijević, Miloš
AU  - Spasojević, Dragica
AU  - Prodanović, Radivoje
AU  - Radotić, Ksenija
AU  - Prodanović, Olivera
PY  - 2018
UR  - http://rimsi.imsi.bg.ac.rs/handle/123456789/1764
AB  - Removal of phenolic compounds from wastewaters was previously studied using different enzymatic approaches. In the presence of hydrogen peroxide, peroxidases are able to oxidize phenol-like compounds and form non-soluble polymers that could be easily removed from aqueous
phase. Horseradish peroxidase (HRP) is the most investigated peroxidase used for phenol removal from waste effluents, but it can be easily inactivated during this process by excess of hydrogen peroxide. In order to increase operational stability of the enzyme, immobilization on different materials and various peroxide delivery systems were tested. In our previous work, we studied bioinspired hydrogels based on natural cell wall polymers and enzymes, for efficient removal of phenols from water. In this work, tyramine-alginate hydrogels that we have previously developed were used for horseradish peroxidase encapsulation within micro-beads obtained in a coupled emulsion polymerization reaction. The aim of this research was to study the influence of tyramine-alginate concentration and hydrogen peroxide delivery system on operational stability and efficiency of phenol removal by immobilized peroxidase. The best result of 96% phenol removal from water solution was achieved by peroxidase immobilized within 20% (w/v) tyramine-alginate micro-beads using delivery system for hydrogen peroxide composed of 0.187 U mL-1 of glucose oxidase and 4 mmol L-1 of glucose. The reusability studies showed that these biocatalysts can be used up to five cycles with slight decrease in their catalytic performance.
PB  - Serbian Plant Physiology Society
PB  - Institute for Biological Research “Siniša Stanković”, University of Belgrade
PB  - Faculty of Biology, University of Belgrade
C3  - 3rd International Conference on Plant Biology (22nd SPPS Meeting)
T1  - Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads
SP  - 157
UR  - https://hdl.handle.net/21.15107/rcub_rimsi_1764
ER  - 
@conference{
author = "Pantić, Nevena and Popović, Nikolina and Prokopijević, Miloš and Spasojević, Dragica and Prodanović, Radivoje and Radotić, Ksenija and Prodanović, Olivera",
year = "2018",
abstract = "Removal of phenolic compounds from wastewaters was previously studied using different enzymatic approaches. In the presence of hydrogen peroxide, peroxidases are able to oxidize phenol-like compounds and form non-soluble polymers that could be easily removed from aqueous
phase. Horseradish peroxidase (HRP) is the most investigated peroxidase used for phenol removal from waste effluents, but it can be easily inactivated during this process by excess of hydrogen peroxide. In order to increase operational stability of the enzyme, immobilization on different materials and various peroxide delivery systems were tested. In our previous work, we studied bioinspired hydrogels based on natural cell wall polymers and enzymes, for efficient removal of phenols from water. In this work, tyramine-alginate hydrogels that we have previously developed were used for horseradish peroxidase encapsulation within micro-beads obtained in a coupled emulsion polymerization reaction. The aim of this research was to study the influence of tyramine-alginate concentration and hydrogen peroxide delivery system on operational stability and efficiency of phenol removal by immobilized peroxidase. The best result of 96% phenol removal from water solution was achieved by peroxidase immobilized within 20% (w/v) tyramine-alginate micro-beads using delivery system for hydrogen peroxide composed of 0.187 U mL-1 of glucose oxidase and 4 mmol L-1 of glucose. The reusability studies showed that these biocatalysts can be used up to five cycles with slight decrease in their catalytic performance.",
publisher = "Serbian Plant Physiology Society, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Faculty of Biology, University of Belgrade",
journal = "3rd International Conference on Plant Biology (22nd SPPS Meeting)",
title = "Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads",
pages = "157",
url = "https://hdl.handle.net/21.15107/rcub_rimsi_1764"
}
Pantić, N., Popović, N., Prokopijević, M., Spasojević, D., Prodanović, R., Radotić, K.,& Prodanović, O.. (2018). Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads. in 3rd International Conference on Plant Biology (22nd SPPS Meeting)
Serbian Plant Physiology Society., 157.
https://hdl.handle.net/21.15107/rcub_rimsi_1764
Pantić N, Popović N, Prokopijević M, Spasojević D, Prodanović R, Radotić K, Prodanović O. Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads. in 3rd International Conference on Plant Biology (22nd SPPS Meeting). 2018;:157.
https://hdl.handle.net/21.15107/rcub_rimsi_1764 .
Pantić, Nevena, Popović, Nikolina, Prokopijević, Miloš, Spasojević, Dragica, Prodanović, Radivoje, Radotić, Ksenija, Prodanović, Olivera, "Optimization of reaction conditions for phenol removal in batch reactor with horseradish peroxidase immobilized within tyramine-alginate micro-beads" in 3rd International Conference on Plant Biology (22nd SPPS Meeting) (2018):157,
https://hdl.handle.net/21.15107/rcub_rimsi_1764 .

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