The enzyme immobilization: Carriers and immobilization methods
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2019
Authors
Spasojević, MilicaProdanović, Olivera

Pantić, Nevena

Popović, Nikolina
Balaž, Ana Marija

Prodanović, Radivoje

Article (Published version)
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Strategies based on the enzyme application are increasingly replacing the conventional chemical procedures because of their efficiency, quicker performance and environmental protection. However, natural enzymes can rarely be used in industry since their beneficial features cannot endure the industrial conditions. Additional drawbacks of natural enzymes are their inhibition by reaction products and difficulty to be removed from the reaction mixture. The most promising technique to substantially improve the enzyme properties, such as activity, pH, thermal and organic-solvent stability, reusability and storage stability, in non-natural environments is by the enzyme immobilization. In this review different techniques used to immobilize enzymes to inert carriers were summarized. Different materials of both the organic and inorganic origin were used as carriers for the enzyme immobilization. A class of new materials where the enzyme performance was enhanced by combining different classical m...aterials and shaping in specific forms was also summarized.
Keywords:
enzymes / immobilization / macroporous / carriers / hydrogelsSource:
Journal of Engineering & Processing Management, 2019, 11, 2, 89-105Publisher:
- Faculty of Technology Zvornik
Funding / projects:
- Application of biomedical engineering for preclinical and clinical practice (RS-41007)
- Allergens, antibodies, enzymes and small physiologically important molecules: design, structure, function and relevance (RS-172049)
- Study of structure-function relationships in the plant cell wall and modifications of the wall structure by enzyme engineering (RS-173017)
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Institut za multidisciplinarna istraživanjaTY - JOUR AU - Spasojević, Milica AU - Prodanović, Olivera AU - Pantić, Nevena AU - Popović, Nikolina AU - Balaž, Ana Marija AU - Prodanović, Radivoje PY - 2019 UR - http://rimsi.imsi.bg.ac.rs/handle/123456789/1757 AB - Strategies based on the enzyme application are increasingly replacing the conventional chemical procedures because of their efficiency, quicker performance and environmental protection. However, natural enzymes can rarely be used in industry since their beneficial features cannot endure the industrial conditions. Additional drawbacks of natural enzymes are their inhibition by reaction products and difficulty to be removed from the reaction mixture. The most promising technique to substantially improve the enzyme properties, such as activity, pH, thermal and organic-solvent stability, reusability and storage stability, in non-natural environments is by the enzyme immobilization. In this review different techniques used to immobilize enzymes to inert carriers were summarized. Different materials of both the organic and inorganic origin were used as carriers for the enzyme immobilization. A class of new materials where the enzyme performance was enhanced by combining different classical materials and shaping in specific forms was also summarized. PB - Faculty of Technology Zvornik T2 - Journal of Engineering & Processing Management T1 - The enzyme immobilization: Carriers and immobilization methods EP - 105 IS - 2 SP - 89 VL - 11 DO - 10.7251/jepm1902089s ER -
@article{ author = "Spasojević, Milica and Prodanović, Olivera and Pantić, Nevena and Popović, Nikolina and Balaž, Ana Marija and Prodanović, Radivoje", year = "2019", abstract = "Strategies based on the enzyme application are increasingly replacing the conventional chemical procedures because of their efficiency, quicker performance and environmental protection. However, natural enzymes can rarely be used in industry since their beneficial features cannot endure the industrial conditions. Additional drawbacks of natural enzymes are their inhibition by reaction products and difficulty to be removed from the reaction mixture. The most promising technique to substantially improve the enzyme properties, such as activity, pH, thermal and organic-solvent stability, reusability and storage stability, in non-natural environments is by the enzyme immobilization. In this review different techniques used to immobilize enzymes to inert carriers were summarized. Different materials of both the organic and inorganic origin were used as carriers for the enzyme immobilization. A class of new materials where the enzyme performance was enhanced by combining different classical materials and shaping in specific forms was also summarized.", publisher = "Faculty of Technology Zvornik", journal = "Journal of Engineering & Processing Management", title = "The enzyme immobilization: Carriers and immobilization methods", pages = "105-89", number = "2", volume = "11", doi = "10.7251/jepm1902089s" }
Spasojević, M., Prodanović, O., Pantić, N., Popović, N., Balaž, A. M.,& Prodanović, R.. (2019). The enzyme immobilization: Carriers and immobilization methods. in Journal of Engineering & Processing Management Faculty of Technology Zvornik., 11(2), 89-105. https://doi.org/10.7251/jepm1902089s
Spasojević M, Prodanović O, Pantić N, Popović N, Balaž AM, Prodanović R. The enzyme immobilization: Carriers and immobilization methods. in Journal of Engineering & Processing Management. 2019;11(2):89-105. doi:10.7251/jepm1902089s .
Spasojević, Milica, Prodanović, Olivera, Pantić, Nevena, Popović, Nikolina, Balaž, Ana Marija, Prodanović, Radivoje, "The enzyme immobilization: Carriers and immobilization methods" in Journal of Engineering & Processing Management, 11, no. 2 (2019):89-105, https://doi.org/10.7251/jepm1902089s . .